Matches in SemOpenAlex for { <https://semopenalex.org/work/W1974865265> ?p ?o ?g. }
- W1974865265 endingPage "963" @default.
- W1974865265 startingPage "953" @default.
- W1974865265 abstract "The machinery of eukaryotic protein synthesis is found in association with the actin cytoskeleton. A major component of this translational apparatus, which is involved in the shuttling of aa-tRNA, is the actin-binding protein elongation factor 1alpha (EF-1alpha). To investigate the consequences for translation of the interaction of EF-1alpha with F-actin, we have studied the effect of F-actin on the ability of EF-1alpha to bind to aa-tRNA. We demonstrate that binding of EF-1alpha:GTP to aa-tRNA is not pH sensitive with a constant binding affinity of approximately 0.2 microM over the physiological range of pH. However, the sharp pH dependence of binding of EF-1alpha to F-actin is sufficient to shift the binding of EF-1alpha from F-actin to aa-tRNA as pH increases. The ability of EF-1alpha to bind either F-actin or aa-tRNA in competition binding experiments is also consistent with the observation that EF-1alpha's binding to F-actin and aa-tRNA is mutually exclusive. Two pH-sensitive actin-binding sequences in EF-1alpha are identified and are predicted to overlap with the aa-tRNA-binding sites. Our results suggest that pH-regulated recruitment and release of EF-1alpha from actin filaments in vivo will supply a high local concentration of EF-1alpha to facilitate polypeptide elongation by the F-actin-associated translational apparatus." @default.
- W1974865265 created "2016-06-24" @default.
- W1974865265 creator A5002838409 @default.
- W1974865265 creator A5014100567 @default.
- W1974865265 creator A5014115163 @default.
- W1974865265 creator A5022535440 @default.
- W1974865265 creator A5058637832 @default.
- W1974865265 creator A5079001721 @default.
- W1974865265 date "1996-11-15" @default.
- W1974865265 modified "2023-09-30" @default.
- W1974865265 title "F-actin sequesters elongation factor 1alpha from interaction with aminoacyl-tRNA in a pH-dependent reaction." @default.
- W1974865265 cites W147050396 @default.
- W1974865265 cites W1491402596 @default.
- W1974865265 cites W1493329012 @default.
- W1974865265 cites W1493978240 @default.
- W1974865265 cites W1497221394 @default.
- W1974865265 cites W1505024016 @default.
- W1974865265 cites W1505411983 @default.
- W1974865265 cites W1520653386 @default.
- W1974865265 cites W1537415606 @default.
- W1974865265 cites W1538692246 @default.
- W1974865265 cites W1578030761 @default.
- W1974865265 cites W1588145642 @default.
- W1974865265 cites W1943419440 @default.
- W1974865265 cites W1965098419 @default.
- W1974865265 cites W1966601917 @default.
- W1974865265 cites W1966651071 @default.
- W1974865265 cites W1983768617 @default.
- W1974865265 cites W1984053379 @default.
- W1974865265 cites W1986392040 @default.
- W1974865265 cites W1988727796 @default.
- W1974865265 cites W1993119469 @default.
- W1974865265 cites W1993896992 @default.
- W1974865265 cites W1995840185 @default.
- W1974865265 cites W1996223704 @default.
- W1974865265 cites W1997648346 @default.
- W1974865265 cites W2000064612 @default.
- W1974865265 cites W2001644877 @default.
- W1974865265 cites W2001923549 @default.
- W1974865265 cites W2003879700 @default.
- W1974865265 cites W2005734517 @default.
- W1974865265 cites W2016181787 @default.
- W1974865265 cites W2016677470 @default.
- W1974865265 cites W2017684491 @default.
- W1974865265 cites W2018289835 @default.
- W1974865265 cites W2018864172 @default.
- W1974865265 cites W2019018346 @default.
- W1974865265 cites W2024484334 @default.
- W1974865265 cites W2027577851 @default.
- W1974865265 cites W2032199812 @default.
- W1974865265 cites W2032533515 @default.
- W1974865265 cites W2035805550 @default.
- W1974865265 cites W2039540684 @default.
- W1974865265 cites W2041131340 @default.
- W1974865265 cites W2045606678 @default.
- W1974865265 cites W2045957363 @default.
- W1974865265 cites W2046427974 @default.
- W1974865265 cites W2052394197 @default.
- W1974865265 cites W2053876416 @default.
- W1974865265 cites W2059813738 @default.
- W1974865265 cites W2061453525 @default.
- W1974865265 cites W2061691186 @default.
- W1974865265 cites W2063073616 @default.
- W1974865265 cites W2068446924 @default.
- W1974865265 cites W2070360313 @default.
- W1974865265 cites W2072910003 @default.
- W1974865265 cites W2080128188 @default.
- W1974865265 cites W2085711791 @default.
- W1974865265 cites W2090404900 @default.
- W1974865265 cites W2091142270 @default.
- W1974865265 cites W2093115344 @default.
- W1974865265 cites W2094070743 @default.
- W1974865265 cites W2094816576 @default.
- W1974865265 cites W2100837269 @default.
- W1974865265 cites W2103204666 @default.
- W1974865265 cites W2104148149 @default.
- W1974865265 cites W2114956112 @default.
- W1974865265 cites W2115231141 @default.
- W1974865265 cites W2138333118 @default.
- W1974865265 cites W2140783516 @default.
- W1974865265 cites W2395986790 @default.
- W1974865265 doi "https://doi.org/10.1083/jcb.135.4.953" @default.
- W1974865265 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2133385" @default.
- W1974865265 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8922379" @default.
- W1974865265 hasPublicationYear "1996" @default.
- W1974865265 type Work @default.
- W1974865265 sameAs 1974865265 @default.
- W1974865265 citedByCount "112" @default.
- W1974865265 countsByYear W19748652652012 @default.
- W1974865265 countsByYear W19748652652013 @default.
- W1974865265 countsByYear W19748652652014 @default.
- W1974865265 countsByYear W19748652652015 @default.
- W1974865265 countsByYear W19748652652016 @default.
- W1974865265 countsByYear W19748652652017 @default.
- W1974865265 countsByYear W19748652652018 @default.
- W1974865265 countsByYear W19748652652019 @default.
- W1974865265 countsByYear W19748652652020 @default.
- W1974865265 countsByYear W19748652652021 @default.