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• W1974871849 abstract "We review an array of newly developed in situ detection methods that can be used for the qualitative and semi-quantitative measurement of various indices related to oxidative stress. The importance of in situ methods over bulk analysis cannot be overstated when considering the structural and cellular complexity of tissue and the effects of diseases thereof. Indeed, in situ detection allows detection of specific cell types affected or specific localization such that a process affecting only a small fraction of the tissue or cells can be readily visualized. Consequently, a positive signal in situ indicates real levels that cannot be masked by unrelated or compensatory responses in adjacent cells, and corrections can be easily made for the modifications to long-lived proteins during physiological aging. In fact, the damage to extracellular matrix proteins of major vessels, provides a cumulative record of long-term oxidative insult. Yet the same properties that make vessels ideal markers for aging limits their sensitivity to detect disease-specific changes unless in situ techniques are used." @default.
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• W1974871849 date "2000-02-05" @default.
• W1974871849 modified "2023-09-26" @default.
• W1974871849 title "Histochemical and Immunocytochemical Approaches to the Study of Oxidative Stress" @default.
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• W1974871849 doi "https://doi.org/10.1515/cclm.2000.015" @default.
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