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• W1974903764 abstract "Abstract— Intact olfactory bulbs from 8‐ to 15‐day‐old mice were compared to slices of olfactory bulb and cerebral hemisphere with respect to uptake of amino acids, respiratory rate, levels of ATP, retention of sodium and potassium, and extracellular space. The uptake of amino acids was lower in intact bulbs than in slice preparations, both in regard to initial rates of uptake and to final steady state levels, at external amino acid concentrations from 0·2 to 2·0mM. Uptake was lower in bulbs attached to brain than in those separated from it and somewhat higher in the half of the bulb closer to the cut surface. In all preparations the uptake of glutamic acid and glycine was highest, uptake of histidine and valine was intermediate, and uptake of lysine was lowest. These differences between intact bulbs and slices could not be correlated with differences in respiratory rate, levels of ATP, or changes in levels of Na + or K + ions. Increases in dextran and inulin spaces, however, were greatest in preparations having the highest rates of amino acid uptake. Although for several amino acids the maximal velocity of uptake (V max ) was 4‐fold higher in slices of bulb than in intact bulbs, the affinity of amino acids to their carrier systems ( K m ) was similar, an indication that the same transport process was operative in both cases. On the basis of these results we propose that intact olfactory bulbs incubated in vitro possess a regulatory mechanism for the limitation of amino acid uptake that is absent or diminished in slices." @default.
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• W1974903764 date "1973-04-01" @default.
• W1974903764 modified "2023-10-09" @default.
• W1974903764 title "THE UPTAKE OF AMINO ACIDS BY THE INTACT OLFACTORY BULB OF THE MOUSE: A COMPARISON WITH TISSUE SLICE PREPARATIONS" @default.
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• W1974903764 doi "https://doi.org/10.1111/j.1471-4159.1973.tb00087.x" @default.
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