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• W1974955393 abstract "Genotyping of sand fly species circulating in Peru was established on the basis of PCR-restriction fragment length polymorphisms (RFLPs) of the 18S ribosomal RNA (rRNA) gene. The sequences of 18S rRNA gene fragments from 12 Lutzomyia and 1 Warileya species were determined and their RFLP-patterns were analyzed. Consequently, RFLP analysis with the restriction enzyme AfaI and then HapII or KpnI, followed by XspI successfully differentiated them. Intraspecific genetic diversity affecting RFLP-patterns was not detected in the specimens collected from 24 areas of 8 departments. The genotyping was applied to the surveillance of sand flies collected from Andean areas where leishmaniasis is endemic, and its usability was verified. The present method promises to be a powerful tool for the classification and surveillance of sand flies circulating in Peru." @default.
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• W1974955393 date "2012-02-01" @default.
• W1974955393 modified "2023-10-14" @default.
• W1974955393 title "Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic" @default.
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• W1974955393 doi "https://doi.org/10.1016/j.actatropica.2011.10.004" @default.
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