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• W1975038057 abstract "No AccessJournal of UrologyInvestigative Urology1 Oct 2004A MALE MURINE MODEL OF PARTIAL BLADDER OUTLET OBSTRUCTION REVEALS CHANGES IN DETRUSOR MORPHOLOGY, CONTRACTILITY AND MYOSIN ISOFORM EXPRESSION J. CHRISTOPHER AUSTIN, SAMUEL K. CHACKO, MICHAEL DiSANTO, DOUGLAS A. CANNING, and STEPHEN A. ZDERIC J. CHRISTOPHER AUSTINJ. CHRISTOPHER AUSTIN More articles by this author , SAMUEL K. CHACKOSAMUEL K. CHACKO More articles by this author , MICHAEL DiSANTOMICHAEL DiSANTO More articles by this author , DOUGLAS A. CANNINGDOUGLAS A. CANNING More articles by this author , and STEPHEN A. ZDERICSTEPHEN A. ZDERIC More articles by this author View All Author Informationhttps://doi.org/10.1097/01.ju.0000138045.61378.96AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Mice with gene deletion or targeted over expression are important for understanding the remodeling that follows partial bladder outlet obstruction (PBOO). This condition predominates in males. We produced PBOO in male mice and now report the physiological, histological and molecular consequences. Materials and Methods: Male C57bl/6 mice were surgically obstructed or subjected to sham surgery and unoperated mice served as controls. Four weeks later the bladders were excised and their function was assessed with in vitro whole bladder cystometry. The optimum volume for pressure generation was determined and isometric pressures were measured for field stimulation and depolarization with KCl. Bladder hypertrophy was classified as severe—bladder mass greater than 50 mg or mild—bladder mass less than 50 mg. The percent muscle fraction was determined by histological analyses. The expression of C-terminal (SM1 and SM2) and N-terminal (SM-B and SM-A) isoforms of myosin heavy chain was analyzed by reverse transcriptase-polymerase chain reaction. Results: Severely hypertrophied bladders had larger optimum volume (p >0.001) and generated less pressure in response to field stimulation (p >0.001) and KCl (p >0.01) with a slower rate of pressure generation than controls or sham operated mice. Increased SM1-to-SM2 and SM-A-to-SM-B ratios were noted in severely obstructed bladders relative to controls or sham operated mice (p <0.05). The muscle fraction decreased slightly in the severely hypertrophied group (p not significant). Conclusions: Our male mouse model of PBOO demonstrates an increase in bladder mass, larger capacity and significantly decreased pressure generation in the in vitro whole bladder model. Obstruction induced increases in the expression of C-terminal (SM1) and N-terminal (SM-A) myosin heavy chain isoforms. References 1 : Voiding function: relevant anatomy, physiology, pharmacology, and molecular aspects. In: Adult and Pediatric Urology. Edited by . Philadelphia: Lippincott Publishers2002: 1061. Google Scholar 2 : Alterations in the physiological properties of urinary bladder smooth muscle caused by bladder emptying against an obstruction. Scand J Urol Nephrol Suppl1997; 184: 51. Google Scholar 3 : Acute biochemical and functional alterations in the partially obstructed rabbit urinary bladder. J Urol1986; 136: 1324. Link, Google Scholar 4 : The guinea pig as a model of gradual urethral obstruction. J Urol1991; 145: 854. 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Google Scholar 12 : Role of myosin isoforms in smooth muscle function. Comp Biochem Physiol B Biochem Mol Biol1997; 117: 1. Google Scholar 13 : Loss of SM-B myosin affects muscle shortening velocity and maximal force development. Nat Cell Biol2001; 3: 1025. Google Scholar 14 : Regional variation in myosin isoforms and phosphorylation at the resting tone in urinary bladder smooth muscle. Am J Physiol Cell Physiol2001; 280: C254. Google Scholar 15 : The smooth muscle myosin seven amino acid heavy chain insert's kinetic role in the crossbridge cycle for mouse bladder. J Physiol2003; 547: 463. Google Scholar 16 : Contractile protein changes in urinary bladder smooth muscle following outlet obstruction. Adv Exptl Med Biol1999; 462: 137. Google Scholar 17 : Alteration in expression of myosin isoforms in detrusor smooth muscle following bladder outlet obstruction. Am J Physiol Cell Physiol2003; 285: C1397. Google Scholar 18 : Effect of partial outlet obstruction on rabbit urinary bladder smooth muscle function. Am J Physiol Renal Physiol2003; 284: F644. Google Scholar From the Division of Urology Children's Hospital of Philadelphia (JCA, DAC, SAZ) and Department of Urology, University of Pennsylvania School of Medicine (SKC, MD), Philadelphia, Pennsylvania© 2004 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byWoo L, Tanaka S, Anumanthan G, Pope J, Thomas J, Adams M, Brock J and Bhowmick N (2011) Mesenchymal Stem Cell Recruitment and Improved Bladder Function After Bladder Outlet Obstruction: Preliminary DataJournal of Urology, VOL. 185, NO. 3, (1132-1138), Online publication date: 1-Mar-2011.Anumanthan G, Tanaka S, Adams C, Thomas J, Wills M, Adams M, Hayward S, Matusik R, Bhowmick N, Brock J and Pope J (2009) Bladder Stromal Loss of Transforming Growth Factor Receptor II Decreases Fibrosis After Bladder ObstructionJournal of Urology, VOL. 182, NO. 4S, (1775-1780), Online publication date: 1-Oct-2009.Myers J, Dall'Era J, Koul S, Kumar B, Khandrika L, Flynn B and Koul H (2009) Biochemical Alterations in Partial Bladder Outlet Obstruction in Mice: Up-Regulation of the Mitogen Activated Protein Kinase PathwayJournal of Urology, VOL. 181, NO. 4, (1926-1931), Online publication date: 1-Apr-2009.Wei W, Howard P, Zderic S and Macarak E (2008) β and γ-Sarcoglycans are Decreased in the Detrusor Smooth Muscle Cells of the Partially Obstructed Rabbit BladderJournal of Urology, VOL. 179, NO. 5, (2052-2056), Online publication date: 1-May-2008. Volume 172Issue 4 Part 1October 2004Page: 1524-1528 Advertisement Copyright & Permissions© 2004 by American Urological Association, Inc.Keywordsmicemyosin heavy chainsbladder neck obstructionbladderMetricsAuthor Information J. CHRISTOPHER AUSTIN More articles by this author SAMUEL K. CHACKO More articles by this author MICHAEL DiSANTO More articles by this author DOUGLAS A. CANNING More articles by this author STEPHEN A. ZDERIC More articles by this author Expand All Advertisement PDF downloadLoading ..." @default.
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• W1975038057 title "A MALE MURINE MODEL OF PARTIAL BLADDER OUTLET OBSTRUCTION REVEALS CHANGES IN DETRUSOR MORPHOLOGY, CONTRACTILITY AND MYOSIN ISOFORM EXPRESSION" @default.
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