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• W1975060596 abstract "ABSTRACT Little is known about the replication cycle of archaeal viruses. We have investigated the ultrastructural changes of Sulfolobus solfataricus P2 associated with infection by Sulfolobus t urreted i cosahedral v irus (STIV). A time course of a near synchronous STIV infection was analyzed using both scanning and transmission electron microscopy. Assembly of STIV particles, including particles lacking DNA, was observed within cells, and fully assembled STIV particles were visible by 30 h postinfection (hpi). STIV was determined to be a lytic virus, causing cell disruption beginning at 30 hpi. Prior to cell lysis, virus infection resulted in the formation of pyramid-like projections from the cell surface. These projections, which have not been documented in any other host-virus system, appeared to be caused by the protrusion of the cell membrane beyond the bordering S-layer. These structures are thought to be sites at which progeny virus particles are released from infected cells. Based on these observations of lysis, a plaque assay was developed for STIV. From these studies we propose an overall assembly model for STIV." @default.
• W1975060596 created "2016-06-24" @default.
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• W1975060596 date "2009-06-15" @default.
• W1975060596 modified "2023-10-03" @default.
• W1975060596 title "Particle Assembly and Ultrastructural Features Associated with Replication of the Lytic Archaeal Virus <i>Sulfolobus</i> Turreted Icosahedral Virus" @default.
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• W1975060596 doi "https://doi.org/10.1128/jvi.02668-08" @default.
• W1975060596 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2687396" @default.
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