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- W1975111870 abstract "E. coli tRNAfMet was labeled specifically with two well-known fluorescent groups, derivatives of anthranilic acid and of dimethylaminonaphthalene sulfonic acid. These were attached through a pyrophosphate linkage to the 5′-end of tRNA similar to the synthesis of methyl pyrophosphate tRNA described by RajBhandary et al. (9). The modification is specific for the 5′-end. The yields in this reaction are moderate (up to 20%). The modified tRNAfMet preparations can still be aminoacylated. The fluorescence measurements showed a certain hydrophobic character of the local environment of the fluorescent moiety." @default.
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- W1975111870 date "1973-03-01" @default.
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- W1975111870 title "Covalent attachment of fluorescent groups to the 5′-end of transfer RNA" @default.
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- W1975111870 doi "https://doi.org/10.1016/s0003-9861(73)80010-4" @default.
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