Matches in SemOpenAlex for { <https://semopenalex.org/work/W1975217115> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W1975217115 endingPage "582" @default.
- W1975217115 startingPage "573" @default.
- W1975217115 abstract "Urine from Sd(a+) individuals was found to contain a beta-N-acetylgalactosaminyltransferase that transfers N-acetylgalactosamine (GalNAc) from UDP-GalNAc to 3'-sialyllactose and glycoproteins carrying the terminal NeuAc alpha-3Gal beta group. This enzyme has been purified 174-fold by affinity chromatography on Blue Sepharose and DEAE-Sephacel chromatography in a yield of 33%. Neither endogenous incorporation nor sugar nucleotide degrading enzymes were found in the purified preparation. The transferase had a pH optimum of pH 7.5 and a requirement for Mn2+ but not for detergents. The Km for UDP-GalNAc was 66 X 10(-6) M, using fetuin as an acceptor. Like beta-GalNAc-transferase from other sources the urinary enzyme had a strict requirement for sialylated acceptors. On the basis of enzymatic and chemical treatment of the product obtained by the transfer of [3H]GalNAc to 3'-sialyllactose, we propose that the enzyme attaches GalNAc in beta-anomeric configuration to O-4 of the galactose residue that is substituted at O-3 by sialic acid. A preparation of Tamm-Horsfall glycoprotein from a Sd(a-) donor lacking beta-GalNAc was found to be the best acceptor among the glycoproteins tested. Studies on the transferase activity toward fetuin, human chorionic gonadotropin, and glycophorin A indicated that the enzyme preferentially adds the sugar to the sialylated terminal end of N-linked oligosaccharides. Unlike the beta-GalNAc-transferase bound to human kidney microsomes (F. Piller et al. (1986) Carbohydr. Res. 149, 171-184) the urinary transferase is able to transfer beta-GalNAc to the NeuAc alpha-3Gal beta-3(NeuAc alpha-6)GalNAc chains bound to the native glycophorin." @default.
- W1975217115 created "2016-06-24" @default.
- W1975217115 creator A5007636414 @default.
- W1975217115 creator A5009615069 @default.
- W1975217115 creator A5046387992 @default.
- W1975217115 date "1988-11-01" @default.
- W1975217115 modified "2023-09-26" @default.
- W1975217115 title "Characterization and partial purification of β-N-acetylgalactosaminyltransferase from urine of Sd(a+) individuals" @default.
- W1975217115 cites W1483729260 @default.
- W1975217115 cites W1497259627 @default.
- W1975217115 cites W1504533936 @default.
- W1975217115 cites W1524769057 @default.
- W1975217115 cites W1559553016 @default.
- W1975217115 cites W1580023435 @default.
- W1975217115 cites W1775749144 @default.
- W1975217115 cites W1917791732 @default.
- W1975217115 cites W1965130705 @default.
- W1975217115 cites W1974282229 @default.
- W1975217115 cites W1989506762 @default.
- W1975217115 cites W1989989027 @default.
- W1975217115 cites W2003850645 @default.
- W1975217115 cites W2010102741 @default.
- W1975217115 cites W2011881328 @default.
- W1975217115 cites W2012128953 @default.
- W1975217115 cites W2032260583 @default.
- W1975217115 cites W2036396415 @default.
- W1975217115 cites W2060905354 @default.
- W1975217115 cites W2070085716 @default.
- W1975217115 cites W2087115659 @default.
- W1975217115 cites W2090399154 @default.
- W1975217115 cites W2100837269 @default.
- W1975217115 cites W2113228804 @default.
- W1975217115 cites W2131684112 @default.
- W1975217115 cites W2141619527 @default.
- W1975217115 cites W2261742712 @default.
- W1975217115 cites W2396729910 @default.
- W1975217115 cites W30305005 @default.
- W1975217115 cites W322562448 @default.
- W1975217115 doi "https://doi.org/10.1016/0003-9861(88)90290-1" @default.
- W1975217115 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3142362" @default.
- W1975217115 hasPublicationYear "1988" @default.
- W1975217115 type Work @default.
- W1975217115 sameAs 1975217115 @default.
- W1975217115 citedByCount "32" @default.
- W1975217115 countsByYear W19752171152013 @default.
- W1975217115 countsByYear W19752171152014 @default.
- W1975217115 countsByYear W19752171152020 @default.
- W1975217115 countsByYear W19752171152022 @default.
- W1975217115 crossrefType "journal-article" @default.
- W1975217115 hasAuthorship W1975217115A5007636414 @default.
- W1975217115 hasAuthorship W1975217115A5009615069 @default.
- W1975217115 hasAuthorship W1975217115A5046387992 @default.
- W1975217115 hasConcept C108625454 @default.
- W1975217115 hasConcept C161019410 @default.
- W1975217115 hasConcept C179247698 @default.
- W1975217115 hasConcept C181199279 @default.
- W1975217115 hasConcept C185592680 @default.
- W1975217115 hasConcept C2776376580 @default.
- W1975217115 hasConcept C2777313579 @default.
- W1975217115 hasConcept C2778322149 @default.
- W1975217115 hasConcept C2778541144 @default.
- W1975217115 hasConcept C2779881004 @default.
- W1975217115 hasConcept C2780115458 @default.
- W1975217115 hasConcept C55493867 @default.
- W1975217115 hasConceptScore W1975217115C108625454 @default.
- W1975217115 hasConceptScore W1975217115C161019410 @default.
- W1975217115 hasConceptScore W1975217115C179247698 @default.
- W1975217115 hasConceptScore W1975217115C181199279 @default.
- W1975217115 hasConceptScore W1975217115C185592680 @default.
- W1975217115 hasConceptScore W1975217115C2776376580 @default.
- W1975217115 hasConceptScore W1975217115C2777313579 @default.
- W1975217115 hasConceptScore W1975217115C2778322149 @default.
- W1975217115 hasConceptScore W1975217115C2778541144 @default.
- W1975217115 hasConceptScore W1975217115C2779881004 @default.
- W1975217115 hasConceptScore W1975217115C2780115458 @default.
- W1975217115 hasConceptScore W1975217115C55493867 @default.
- W1975217115 hasIssue "2" @default.
- W1975217115 hasLocation W19752171151 @default.
- W1975217115 hasLocation W19752171152 @default.
- W1975217115 hasOpenAccess W1975217115 @default.
- W1975217115 hasPrimaryLocation W19752171151 @default.
- W1975217115 hasRelatedWork W1569012045 @default.
- W1975217115 hasRelatedWork W1676407394 @default.
- W1975217115 hasRelatedWork W1730217395 @default.
- W1975217115 hasRelatedWork W1965605354 @default.
- W1975217115 hasRelatedWork W1975217115 @default.
- W1975217115 hasRelatedWork W1992941546 @default.
- W1975217115 hasRelatedWork W2088265457 @default.
- W1975217115 hasRelatedWork W2759220 @default.
- W1975217115 hasRelatedWork W426835635 @default.
- W1975217115 hasRelatedWork W2099781209 @default.
- W1975217115 hasVolume "266" @default.
- W1975217115 isParatext "false" @default.
- W1975217115 isRetracted "false" @default.
- W1975217115 magId "1975217115" @default.
- W1975217115 workType "article" @default.