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- W1975230535 abstract "利用前期克隆的小麦超氧化物歧化酶(SOD)基因TaSOD1.1和TaSOD1.2,构建融合上述基因编码阅读框(ORF)的双元表达载体。采用农杆菌介导的遗传转化技术,获得了过量表达TaSOD1.1和TaSOD1.2的转基因烟草植株。研究表明,NaCl处理下,与对照相比,转基因植株伤害较小,叶片失绿面积较少,植株长势明显增强,叶片的SOD活性均明显提高,而MDA含量明显降低。转基因植株的叶绿素a、叶绿素b和类胡萝卜素含量,以及可溶性糖和可溶性蛋白含量也表现与SOD活性相同的趋势。表明在烟草中高表达小麦TaSOD1.1和TaSOD1.2基因,通过外源基因在转录水平上的调节,能增加植株SOD活性,减轻盐分胁迫造成的细胞膜质过氧化,增强植株抵御盐分胁迫的能力。" @default.
- W1975230535 created "2016-06-24" @default.
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- W1975230535 date "2008-11-30" @default.
- W1975230535 modified "2023-09-26" @default.
- W1975230535 title "Effects of Overexpression of Wheat Superoxide Dismutase (SOD) Genes on Salt Tolerant Capability in Tobacco" @default.
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- W1975230535 doi "https://doi.org/10.3724/sp.j.1006.2008.01403" @default.
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