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- W1975314191 abstract "An automated high-performance liquid chromatographic method for the direct injection of diltiazem plasma samples has been developed. It involves automatic injection of plasma samples (200μl) on a C18 pro-column (40 μm), clean-up of the pre-column with water to remove proteins and salts and transfer of the analytes to the analytical column. During the chromatographic process, the pre-column is reset with respect to the analytical column and flushed with different solvents to remove endogenous contaminants. The analysis is performed on a C18 column coupled to an ultraviolet detector. The whole process (on-line clean-up and chromatography) takes ca. 12 min and is completely automated. The detection limit of the method is ca. 2 ng/ml with 200-μl aliquots of plasma sample. Very good results were obtained for the day-to-day and within-day reproducibilities (5.9 and 4.3%, respectively, in the concentration range 10–200 ng/ml in plasma)." @default.
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- W1975314191 date "1987-01-01" @default.
- W1975314191 modified "2023-09-25" @default.
- W1975314191 title "Automated high-performance liquid chromatographic and column-switching technique for on-line clean-up and analysis of diltiazem in human plasma" @default.
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- W1975314191 doi "https://doi.org/10.1016/0378-4347(87)80347-x" @default.
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