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- W1975323817 abstract "In the present study, a mutated chimeric orf364 , named orf366-c , was identified in a maize C type cytoplasmic male sterile line (CMS-C) by PCR and RT-PCR. Four base pair adjacent base substitution mutations (CAAA to TTTT) and eight bp insertion mutations were found in orf366-c compared with orf364 . ORF366-C was predicted to contain one membrane-spanning domain using TMHMM online software. Real-time quantitative PCR analysis showed that orf366-c was upregulated in the CMS-C line in comparison to its maintainer line at the uninucleate stage. The protokaryotic protein expression of orf366-c in Escherichia coli showed that ORF366-C could be a cytotoxic protein. All the results indicated that orf366-c may be associated with maize CMS-C." @default.
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- W1975323817 date "2013-05-18" @default.
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- W1975323817 title "Study on the expression of specific chimeric-orfs of C type cytoplasmic male sterile in maize" @default.
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- W1975323817 doi "https://doi.org/10.1080/07929978.2014.945314" @default.
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