Matches in SemOpenAlex for { <https://semopenalex.org/work/W1975344409> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W1975344409 endingPage "642" @default.
- W1975344409 startingPage "637" @default.
- W1975344409 abstract "Rat transferrin (rTf), containing one complex glycan, and human transferrin (hTf), containing two complex glycans, were treated with peptide:N-glycosidase F (PNGase) under nondenaturing conditions. Apo rTf with a nonfucosylated standard biantennary glycan, but not its diferric counterpart, yielded satisfactory amounts (approximately 55% in 7 h) of aglyco Tf (AgTf). The presence of a chitobiose core fucose reduced yields to approximately 30%, whereas an additional NeuAc on the GlcNAc in the Man(alpha 1-3) branch had no adverse effect. Triton X-100 impaired deglycosylation. The main product (approximately 65%) obtained from apo hTf was monoglyco Tf (MgTf). Analysis of the cyanogen bromide fragments of MgTf revealed that PNGase did not discriminate between the two glycosylation sites of hTf. A negligible portion (2-4%) of AgTf, that was also obtained during the reaction, probably resulted from PNGase action on denatured hTf molecules. Modified Tfs were separated by affinity chromatography, radiolabeled, mixed with another preparation of interest, and injected in rats. Total-body radiation measurements showed that the half-life of rat AgTf was 19-20% shorter than that of rTf but 9% longer than that of asialo Tf. This suggests that close to 76% of the change in the degradative rate observed after desialylating rTf is referable to charge loss rather than the exposure of Gal residues. Human MgTf was catabolized by rats 8-9% faster than the parent protein, while human AgTf behaved in vivo like a denatured protein. It is concluded that sialyl carboxyls are a codeterminant of the normal lifetime of transferrins." @default.
- W1975344409 created "2016-06-24" @default.
- W1975344409 creator A5032831310 @default.
- W1975344409 creator A5078077973 @default.
- W1975344409 creator A5087055847 @default.
- W1975344409 date "1989-02-01" @default.
- W1975344409 modified "2023-09-27" @default.
- W1975344409 title "Rat aglycotransferrin and human monoglycotransferrin: Production and metabolic properties" @default.
- W1975344409 cites W132806077 @default.
- W1975344409 cites W1499197499 @default.
- W1975344409 cites W1503231586 @default.
- W1975344409 cites W1518721573 @default.
- W1975344409 cites W1569589672 @default.
- W1975344409 cites W1575133603 @default.
- W1975344409 cites W1586521119 @default.
- W1975344409 cites W1601992608 @default.
- W1975344409 cites W1790113892 @default.
- W1975344409 cites W1985149002 @default.
- W1975344409 cites W1995346382 @default.
- W1975344409 cites W2019465667 @default.
- W1975344409 cites W2031119708 @default.
- W1975344409 cites W2035995596 @default.
- W1975344409 cites W2057035627 @default.
- W1975344409 cites W2080532638 @default.
- W1975344409 cites W2099632503 @default.
- W1975344409 cites W2100837269 @default.
- W1975344409 cites W2119154667 @default.
- W1975344409 cites W2175128997 @default.
- W1975344409 cites W2341056240 @default.
- W1975344409 cites W2411493629 @default.
- W1975344409 doi "https://doi.org/10.1016/0003-9861(89)90331-7" @default.
- W1975344409 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2913950" @default.
- W1975344409 hasPublicationYear "1989" @default.
- W1975344409 type Work @default.
- W1975344409 sameAs 1975344409 @default.
- W1975344409 citedByCount "10" @default.
- W1975344409 countsByYear W19753444092012 @default.
- W1975344409 countsByYear W19753444092016 @default.
- W1975344409 countsByYear W19753444092023 @default.
- W1975344409 crossrefType "journal-article" @default.
- W1975344409 hasAuthorship W1975344409A5032831310 @default.
- W1975344409 hasAuthorship W1975344409A5078077973 @default.
- W1975344409 hasAuthorship W1975344409A5087055847 @default.
- W1975344409 hasConcept C104317684 @default.
- W1975344409 hasConcept C108625454 @default.
- W1975344409 hasConcept C167625842 @default.
- W1975344409 hasConcept C185592680 @default.
- W1975344409 hasConcept C206212055 @default.
- W1975344409 hasConcept C22814914 @default.
- W1975344409 hasConcept C2776817266 @default.
- W1975344409 hasConcept C2777093645 @default.
- W1975344409 hasConcept C2777313579 @default.
- W1975344409 hasConcept C2779281246 @default.
- W1975344409 hasConcept C2780223390 @default.
- W1975344409 hasConcept C43617362 @default.
- W1975344409 hasConcept C55493867 @default.
- W1975344409 hasConceptScore W1975344409C104317684 @default.
- W1975344409 hasConceptScore W1975344409C108625454 @default.
- W1975344409 hasConceptScore W1975344409C167625842 @default.
- W1975344409 hasConceptScore W1975344409C185592680 @default.
- W1975344409 hasConceptScore W1975344409C206212055 @default.
- W1975344409 hasConceptScore W1975344409C22814914 @default.
- W1975344409 hasConceptScore W1975344409C2776817266 @default.
- W1975344409 hasConceptScore W1975344409C2777093645 @default.
- W1975344409 hasConceptScore W1975344409C2777313579 @default.
- W1975344409 hasConceptScore W1975344409C2779281246 @default.
- W1975344409 hasConceptScore W1975344409C2780223390 @default.
- W1975344409 hasConceptScore W1975344409C43617362 @default.
- W1975344409 hasConceptScore W1975344409C55493867 @default.
- W1975344409 hasIssue "2" @default.
- W1975344409 hasLocation W19753444091 @default.
- W1975344409 hasLocation W19753444092 @default.
- W1975344409 hasOpenAccess W1975344409 @default.
- W1975344409 hasPrimaryLocation W19753444091 @default.
- W1975344409 hasRelatedWork W1965518968 @default.
- W1975344409 hasRelatedWork W1975019028 @default.
- W1975344409 hasRelatedWork W2005361276 @default.
- W1975344409 hasRelatedWork W2035111936 @default.
- W1975344409 hasRelatedWork W2054338311 @default.
- W1975344409 hasRelatedWork W2064872101 @default.
- W1975344409 hasRelatedWork W2079418606 @default.
- W1975344409 hasRelatedWork W2095198525 @default.
- W1975344409 hasRelatedWork W2973851721 @default.
- W1975344409 hasRelatedWork W3036978204 @default.
- W1975344409 hasVolume "268" @default.
- W1975344409 isParatext "false" @default.
- W1975344409 isRetracted "false" @default.
- W1975344409 magId "1975344409" @default.
- W1975344409 workType "article" @default.