FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1975387938> ?p ?o ?g. }

• W1975387938 endingPage "e63245" @default.
• W1975387938 startingPage "e63245" @default.
• W1975387938 abstract "We have previously reported that the mitochondria inhibitor 3-nitropropionic acid (3-NP), induces the expression of DNA damage-regulated autophagy modulator1 (DRAM1) and activation of autophagy in rat striatum. Although the role of DRAM1 in autophagy has been previously characterized, the detailed mechanism by which DRAM1 regulates autophagy activity has not been fully understood. The present study investigated the role of DRAM1 in regulating autophagy flux. In A549 cells expressing wilt-type TP53, 3-NP increased the protein levels of DRAM1 and LC3-II, whereas decreased the levels of SQSTM1 (sequestosome 1). The increase in LC3-II and decrease in SQSTM1 were blocked by the autophagy inhibitor 3-methyl-adenine. Lack of TP53 or knock-down of TP53 in cells impaired the induction of DRAM1. Knock-down of DRAM1 with siRNA significantly reduced 3-NP-induced upregulation of LC3-II and downregulation of SQSTM1, indicating DRAM1 contributes to autophagy activation. Knock-down of DRAM1 robustly decreased rate of disappearance of induced autophagosomes, increased RFP-LC3 fluorescence dots and decreased the decline of LC3-II after withdraw of rapamycin, indicating DRAM1 promotes autophagy flux. DRAM1 siRNA inhibited lysosomal V-ATPase and acidification of lysosomes. As a result, DRAM1 siRNA reduced activation of lysosomal cathepsin D. Similar to DRAM1 siRNA, lysosomal inhibitors E64d and chloroquine also inhibited clearance of autophagosomes and activation of lysosomal cathapsin D after 3-NP treatment. These data suggest that DRAM1 plays important roles in autophagy activation induced by mitochondria dysfunction. DRAM1 affects autophagy through argument of lysosomal acidification, fusion of lysosomes with autophagosomes and clearance of autophagosomes." @default.
• W1975387938 created "2016-06-24" @default.
• W1975387938 creator A5002025384 @default.
• W1975387938 creator A5008056710 @default.
• W1975387938 creator A5047881540 @default.
• W1975387938 creator A5085174800 @default.
• W1975387938 date "2013-05-17" @default.
• W1975387938 modified "2023-10-11" @default.
• W1975387938 title "DRAM1 Regulates Autophagy Flux through Lysosomes" @default.
• W1975387938 cites W1550693054 @default.
• W1975387938 cites W1561924747 @default.
• W1975387938 cites W1563847798 @default.
• W1975387938 cites W1856333849 @default.
• W1975387938 cites W1969629251 @default.
• W1975387938 cites W1972296376 @default.
• W1975387938 cites W1977767269 @default.
• W1975387938 cites W1978310592 @default.
• W1975387938 cites W1982074683 @default.
• W1975387938 cites W1990834080 @default.
• W1975387938 cites W1993996853 @default.
• W1975387938 cites W1994817218 @default.
• W1975387938 cites W2001197606 @default.
• W1975387938 cites W2001591494 @default.
• W1975387938 cites W2004355943 @default.
• W1975387938 cites W2010859456 @default.
• W1975387938 cites W2023288915 @default.
• W1975387938 cites W2025714022 @default.
• W1975387938 cites W2035115523 @default.
• W1975387938 cites W2041090873 @default.
• W1975387938 cites W2049387807 @default.
• W1975387938 cites W2050869877 @default.
• W1975387938 cites W2056504998 @default.
• W1975387938 cites W2063761428 @default.
• W1975387938 cites W2076269247 @default.
• W1975387938 cites W2080973723 @default.
• W1975387938 cites W2089952244 @default.
• W1975387938 cites W2103235619 @default.
• W1975387938 cites W2111607026 @default.
• W1975387938 cites W2135574023 @default.
• W1975387938 cites W2142120946 @default.
• W1975387938 cites W2146124002 @default.
• W1975387938 cites W2150027419 @default.
• W1975387938 cites W2158124942 @default.
• W1975387938 cites W2167656141 @default.
• W1975387938 cites W3193089118 @default.
• W1975387938 cites W4366065638 @default.
• W1975387938 doi "https://doi.org/10.1371/journal.pone.0063245" @default.
• W1975387938 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3656954" @default.
• W1975387938 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23696801" @default.
• W1975387938 hasPublicationYear "2013" @default.
• W1975387938 type Work @default.
• W1975387938 sameAs 1975387938 @default.
• W1975387938 citedByCount "70" @default.
• W1975387938 countsByYear W19753879382013 @default.
• W1975387938 countsByYear W19753879382014 @default.
• W1975387938 countsByYear W19753879382015 @default.
• W1975387938 countsByYear W19753879382016 @default.
• W1975387938 countsByYear W19753879382017 @default.
• W1975387938 countsByYear W19753879382018 @default.
• W1975387938 countsByYear W19753879382019 @default.
• W1975387938 countsByYear W19753879382020 @default.
• W1975387938 countsByYear W19753879382021 @default.
• W1975387938 countsByYear W19753879382022 @default.
• W1975387938 countsByYear W19753879382023 @default.
• W1975387938 crossrefType "journal-article" @default.
• W1975387938 hasAuthorship W1975387938A5002025384 @default.
• W1975387938 hasAuthorship W1975387938A5008056710 @default.
• W1975387938 hasAuthorship W1975387938A5047881540 @default.
• W1975387938 hasAuthorship W1975387938A5085174800 @default.
• W1975387938 hasBestOaLocation W19753879381 @default.
• W1975387938 hasConcept C104317684 @default.
• W1975387938 hasConcept C127561419 @default.
• W1975387938 hasConcept C161478664 @default.
• W1975387938 hasConcept C167844969 @default.
• W1975387938 hasConcept C178790620 @default.
• W1975387938 hasConcept C181199279 @default.
• W1975387938 hasConcept C185592680 @default.
• W1975387938 hasConcept C190283241 @default.
• W1975387938 hasConcept C203522944 @default.
• W1975387938 hasConcept C2777722557 @default.
• W1975387938 hasConcept C2780216420 @default.
• W1975387938 hasConcept C28859421 @default.
• W1975387938 hasConcept C2908656506 @default.
• W1975387938 hasConcept C55493867 @default.
• W1975387938 hasConcept C68709404 @default.
• W1975387938 hasConcept C79879829 @default.
• W1975387938 hasConcept C86803240 @default.
• W1975387938 hasConcept C95444343 @default.
• W1975387938 hasConceptScore W1975387938C104317684 @default.
• W1975387938 hasConceptScore W1975387938C127561419 @default.
• W1975387938 hasConceptScore W1975387938C161478664 @default.
• W1975387938 hasConceptScore W1975387938C167844969 @default.
• W1975387938 hasConceptScore W1975387938C178790620 @default.
• W1975387938 hasConceptScore W1975387938C181199279 @default.
• W1975387938 hasConceptScore W1975387938C185592680 @default.
• W1975387938 hasConceptScore W1975387938C190283241 @default.
• W1975387938 hasConceptScore W1975387938C203522944 @default.
• W1975387938 hasConceptScore W1975387938C2777722557 @default.

• next