FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1975419092> ?p ?o ?g. }

• W1975419092 abstract "Treatment of calf thyroid slices with TSH increases the nuclease sensitivity of nuclear chromatin, i.e. the amount of DNA released from nuclei by mild digestion with DNase I and micrococcal nuclease. Cycloheximide and alpha-amanitin were used to investigate the roles played by protein and RNA synthesis in mediating this effect of TSH; alpha-difluoromethylornithine, an irreversible inhibitor of ornithine decarboxylase, was used to investigate the possible involvement of polyamines. Calf thyroid slices were incubated with or without TSH (50 mU/ml) for 5 h, in the presence or absence of inhibitors. Nuclei were then prepared, subjected to mild digestion with micrococcal nuclease, and centrifuged at 1200 X g. The amount of DNA in 1200 X g supernatants was increased by TSH; this was inhibited by cycloheximide (100 micrograms/ml) and alpha-amanitin (4 micrograms/ml) when these agents were present throughout incubations with TSH. In contrast, alpha-amanitin failed to inhibit the TSH effect when it was added to incubations 30 min or 2 h after the addition of TSH. These results indicate that RNA and protein synthesis play a part in mediating the effect of TSH on the micrococcal nuclease sensitivity of chromatin, and that the RNA synthesis involved takes place within the first 30 min of exposure of thyroid slices to TSH. alpha-Difluoromethylornithine (5 mM) inhibited the TSH-dependent development of micrococcal nuclease sensitivity; however, it also inhibited nuclease digestion when it was added directly to nuclei prepared from fresh thyroid tissue. This observation should serve as a warning against uncritical acceptance of the notion that all effects of alpha-difluoromethylornithine are the result of inhibition of ornithine decarboxylase." @default.
• W1975419092 created "2016-06-24" @default.
• W1975419092 creator A5006271973 @default.
• W1975419092 creator A5061985455 @default.
• W1975419092 creator A5083911472 @default.
• W1975419092 date "1984-11-01" @default.
• W1975419092 modified "2023-09-25" @default.
• W1975419092 title "Effects of Cycloheximide,α-Amanitin, andα-Difluoromethylornithine on Thyrotropin-Induced Increases in the Micrococcal Nuclease Sensitivity of Thyroid Nuclear Chromatin*" @default.
• W1975419092 cites W1131804799 @default.
• W1975419092 cites W1520436089 @default.
• W1975419092 cites W1557612767 @default.
• W1975419092 cites W1666807167 @default.
• W1975419092 cites W1906197854 @default.
• W1975419092 cites W1965505038 @default.
• W1975419092 cites W1978377354 @default.
• W1975419092 cites W1982069049 @default.
• W1975419092 cites W1984060614 @default.
• W1975419092 cites W2001567674 @default.
• W1975419092 cites W2002520814 @default.
• W1975419092 cites W2008187888 @default.
• W1975419092 cites W2027033860 @default.
• W1975419092 cites W2045553639 @default.
• W1975419092 cites W2051621781 @default.
• W1975419092 cites W2054366578 @default.
• W1975419092 cites W2070226057 @default.
• W1975419092 cites W2071932002 @default.
• W1975419092 cites W2075865306 @default.
• W1975419092 cites W2075969450 @default.
• W1975419092 cites W2086894223 @default.
• W1975419092 cites W2087114818 @default.
• W1975419092 cites W2106716708 @default.
• W1975419092 cites W2168457817 @default.
• W1975419092 cites W2421718131 @default.
• W1975419092 cites W340457347 @default.
• W1975419092 cites W41553241 @default.
• W1975419092 doi "https://doi.org/10.1210/endo-115-5-1705" @default.
• W1975419092 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6436005" @default.
• W1975419092 hasPublicationYear "1984" @default.
• W1975419092 type Work @default.
• W1975419092 sameAs 1975419092 @default.
• W1975419092 citedByCount "2" @default.
• W1975419092 crossrefType "journal-article" @default.
• W1975419092 hasAuthorship W1975419092A5006271973 @default.
• W1975419092 hasAuthorship W1975419092A5061985455 @default.
• W1975419092 hasAuthorship W1975419092A5083911472 @default.
• W1975419092 hasConcept C104317684 @default.
• W1975419092 hasConcept C126322002 @default.
• W1975419092 hasConcept C134018914 @default.
• W1975419092 hasConcept C139024627 @default.
• W1975419092 hasConcept C153911025 @default.
• W1975419092 hasConcept C2777271071 @default.
• W1975419092 hasConcept C2778128677 @default.
• W1975419092 hasConcept C3675279 @default.
• W1975419092 hasConcept C526584372 @default.
• W1975419092 hasConcept C552990157 @default.
• W1975419092 hasConcept C55493867 @default.
• W1975419092 hasConcept C67705224 @default.
• W1975419092 hasConcept C71924100 @default.
• W1975419092 hasConcept C83640560 @default.
• W1975419092 hasConcept C84772758 @default.
• W1975419092 hasConcept C86803240 @default.
• W1975419092 hasConceptScore W1975419092C104317684 @default.
• W1975419092 hasConceptScore W1975419092C126322002 @default.
• W1975419092 hasConceptScore W1975419092C134018914 @default.
• W1975419092 hasConceptScore W1975419092C139024627 @default.
• W1975419092 hasConceptScore W1975419092C153911025 @default.
• W1975419092 hasConceptScore W1975419092C2777271071 @default.
• W1975419092 hasConceptScore W1975419092C2778128677 @default.
• W1975419092 hasConceptScore W1975419092C3675279 @default.
• W1975419092 hasConceptScore W1975419092C526584372 @default.
• W1975419092 hasConceptScore W1975419092C552990157 @default.
• W1975419092 hasConceptScore W1975419092C55493867 @default.
• W1975419092 hasConceptScore W1975419092C67705224 @default.
• W1975419092 hasConceptScore W1975419092C71924100 @default.
• W1975419092 hasConceptScore W1975419092C83640560 @default.
• W1975419092 hasConceptScore W1975419092C84772758 @default.
• W1975419092 hasConceptScore W1975419092C86803240 @default.
• W1975419092 hasLocation W19754190921 @default.
• W1975419092 hasLocation W19754190922 @default.
• W1975419092 hasOpenAccess W1975419092 @default.
• W1975419092 hasPrimaryLocation W19754190921 @default.
• W1975419092 hasRelatedWork W1534168406 @default.
• W1975419092 hasRelatedWork W1858358649 @default.
• W1975419092 hasRelatedWork W1982527021 @default.
• W1975419092 hasRelatedWork W2007075123 @default.
• W1975419092 hasRelatedWork W2074985522 @default.
• W1975419092 hasRelatedWork W2137630666 @default.
• W1975419092 hasRelatedWork W2140580396 @default.
• W1975419092 hasRelatedWork W2355195879 @default.
• W1975419092 hasRelatedWork W2412835573 @default.
• W1975419092 hasRelatedWork W4249613248 @default.
• W1975419092 isParatext "false" @default.
• W1975419092 isRetracted "false" @default.
• W1975419092 magId "1975419092" @default.
• W1975419092 workType "article" @default.