Matches in SemOpenAlex for { <https://semopenalex.org/work/W1975455557> ?p ?o ?g. }
- W1975455557 endingPage "864" @default.
- W1975455557 startingPage "859" @default.
- W1975455557 abstract "The aminoacyl (A site) tRNA analog 4-thio-dT-p-C-p-puromycin (s4TCPm) photochemically cross-links with high efficiency and specificity to G2553 of 23S rRNA and is peptidyl transferase reactive in its cross-linked state, establishing proximity between the highly conserved 2555 loop in domain V of 23S rRNA and the universally conserved CCA end of tRNA. To test for base-pairing interactions between 23S rRNA and aminoacyl tRNA, site-directed mutations were made at the universally conserved nucleotides U2552 and G2553 of 23S rRNA in both E. coli and B. stearothermophilus ribosomal RNA and incorporated into ribosomes. Mutations at G2553 resulted in dominant growth defects in E. coli and in decreased levels of peptidyl transferase activity in vitro. Genetic analysis in vitro of U2552 and G2553 mutant ribosomes and CCA end mutant tRNA substrates identified a base-pairing interaction between C75 of aminoacyl tRNA and G2553 of 23S rRNA." @default.
- W1975455557 created "2016-06-24" @default.
- W1975455557 creator A5050772561 @default.
- W1975455557 creator A5069289020 @default.
- W1975455557 date "1999-11-01" @default.
- W1975455557 modified "2023-10-18" @default.
- W1975455557 title "Base-Pairing between 23S rRNA and tRNA in the Ribosomal A Site" @default.
- W1975455557 cites W1040338230 @default.
- W1975455557 cites W1485726210 @default.
- W1975455557 cites W1508355684 @default.
- W1975455557 cites W1553162372 @default.
- W1975455557 cites W1573582959 @default.
- W1975455557 cites W1829250676 @default.
- W1975455557 cites W1868347993 @default.
- W1975455557 cites W1964386708 @default.
- W1975455557 cites W1964652224 @default.
- W1975455557 cites W1970107043 @default.
- W1975455557 cites W1983700089 @default.
- W1975455557 cites W1988017690 @default.
- W1975455557 cites W1988455121 @default.
- W1975455557 cites W1993667465 @default.
- W1975455557 cites W1995337744 @default.
- W1975455557 cites W2004670728 @default.
- W1975455557 cites W2004862139 @default.
- W1975455557 cites W2012841276 @default.
- W1975455557 cites W2016336609 @default.
- W1975455557 cites W2017771698 @default.
- W1975455557 cites W2020692930 @default.
- W1975455557 cites W2021012126 @default.
- W1975455557 cites W2022082484 @default.
- W1975455557 cites W2022346667 @default.
- W1975455557 cites W2032232957 @default.
- W1975455557 cites W2044905393 @default.
- W1975455557 cites W2045612031 @default.
- W1975455557 cites W2045690036 @default.
- W1975455557 cites W2053524548 @default.
- W1975455557 cites W2069927544 @default.
- W1975455557 cites W2092641944 @default.
- W1975455557 cites W2093091879 @default.
- W1975455557 cites W2098271534 @default.
- W1975455557 cites W2113761741 @default.
- W1975455557 cites W2198612891 @default.
- W1975455557 cites W2755731088 @default.
- W1975455557 cites W4251684055 @default.
- W1975455557 doi "https://doi.org/10.1016/s1097-2765(00)80395-0" @default.
- W1975455557 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10619032" @default.
- W1975455557 hasPublicationYear "1999" @default.
- W1975455557 type Work @default.
- W1975455557 sameAs 1975455557 @default.
- W1975455557 citedByCount "178" @default.
- W1975455557 countsByYear W19754555572012 @default.
- W1975455557 countsByYear W19754555572013 @default.
- W1975455557 countsByYear W19754555572014 @default.
- W1975455557 countsByYear W19754555572015 @default.
- W1975455557 countsByYear W19754555572016 @default.
- W1975455557 countsByYear W19754555572017 @default.
- W1975455557 countsByYear W19754555572018 @default.
- W1975455557 countsByYear W19754555572019 @default.
- W1975455557 countsByYear W19754555572020 @default.
- W1975455557 countsByYear W19754555572021 @default.
- W1975455557 countsByYear W19754555572022 @default.
- W1975455557 countsByYear W19754555572023 @default.
- W1975455557 crossrefType "journal-article" @default.
- W1975455557 hasAuthorship W1975455557A5050772561 @default.
- W1975455557 hasAuthorship W1975455557A5069289020 @default.
- W1975455557 hasBestOaLocation W19754555571 @default.
- W1975455557 hasConcept C104317684 @default.
- W1975455557 hasConcept C106774688 @default.
- W1975455557 hasConcept C107824862 @default.
- W1975455557 hasConcept C153911025 @default.
- W1975455557 hasConcept C153957851 @default.
- W1975455557 hasConcept C185581394 @default.
- W1975455557 hasConcept C2778321597 @default.
- W1975455557 hasConcept C2779200571 @default.
- W1975455557 hasConcept C3675279 @default.
- W1975455557 hasConcept C40749294 @default.
- W1975455557 hasConcept C54355233 @default.
- W1975455557 hasConcept C552990157 @default.
- W1975455557 hasConcept C55493867 @default.
- W1975455557 hasConcept C67705224 @default.
- W1975455557 hasConcept C67905577 @default.
- W1975455557 hasConcept C69080955 @default.
- W1975455557 hasConcept C86803240 @default.
- W1975455557 hasConcept C88478588 @default.
- W1975455557 hasConcept C96267730 @default.
- W1975455557 hasConceptScore W1975455557C104317684 @default.
- W1975455557 hasConceptScore W1975455557C106774688 @default.
- W1975455557 hasConceptScore W1975455557C107824862 @default.
- W1975455557 hasConceptScore W1975455557C153911025 @default.
- W1975455557 hasConceptScore W1975455557C153957851 @default.
- W1975455557 hasConceptScore W1975455557C185581394 @default.
- W1975455557 hasConceptScore W1975455557C2778321597 @default.
- W1975455557 hasConceptScore W1975455557C2779200571 @default.
- W1975455557 hasConceptScore W1975455557C3675279 @default.
- W1975455557 hasConceptScore W1975455557C40749294 @default.
- W1975455557 hasConceptScore W1975455557C54355233 @default.
- W1975455557 hasConceptScore W1975455557C552990157 @default.
- W1975455557 hasConceptScore W1975455557C55493867 @default.