Matches in SemOpenAlex for { <https://semopenalex.org/work/W1975572249> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1975572249 endingPage "144" @default.
- W1975572249 startingPage "135" @default.
- W1975572249 abstract "To determine the primary structure of chitosanase, which was produced by Bacillus circulans MH-K1, its amino acid sequence was analyzed. Total 183 amino acids were determined. Two kinds of primer were synthesized according to the obtained amino acid sequence, and were used for PCR amplification of chitosanase gene. A 620bp fragment was amplified, and was used for a probe for Southern hybridization of the genomic DNA which was cut by some restriction enzymes. A 5.6kb PstI fragment was isolated and introduced into pUC 19 vector. Colonies which harbored chitosanase gene containing pUC 19 were detected by colony hybridization with the probe. HindIII/HindIII fragment (1.2kb) and HindIII/SacI fragment (0.7kb) were sub-cloned and sequenced. The chitosanase gene (open reading frame is 900 by containing 259 amino acids and a signal peptide) was coded by the fragments. There was no meaningful homology to other enzymes including chitinase." @default.
- W1975572249 created "2016-06-24" @default.
- W1975572249 creator A5009916043 @default.
- W1975572249 creator A5010336415 @default.
- W1975572249 creator A5029245450 @default.
- W1975572249 creator A5053778905 @default.
- W1975572249 creator A5078109225 @default.
- W1975572249 creator A5079606283 @default.
- W1975572249 creator A5081958388 @default.
- W1975572249 creator A5085337453 @default.
- W1975572249 date "1992-01-01" @default.
- W1975572249 modified "2023-10-12" @default.
- W1975572249 title "Primary structure of chitosanase produced by Bacillus circulans MH-K1." @default.
- W1975572249 cites W1488243135 @default.
- W1975572249 cites W1545327627 @default.
- W1975572249 cites W1588728917 @default.
- W1975572249 cites W1602629787 @default.
- W1975572249 cites W1863451475 @default.
- W1975572249 cites W1974534791 @default.
- W1975572249 cites W1984380148 @default.
- W1975572249 cites W2007438535 @default.
- W1975572249 cites W2061747050 @default.
- W1975572249 cites W2088282990 @default.
- W1975572249 cites W2091502120 @default.
- W1975572249 cites W2091853951 @default.
- W1975572249 cites W2129709467 @default.
- W1975572249 cites W2144634347 @default.
- W1975572249 cites W2156432065 @default.
- W1975572249 cites W2332809592 @default.
- W1975572249 doi "https://doi.org/10.2323/jgam.38.135" @default.
- W1975572249 hasPublicationYear "1992" @default.
- W1975572249 type Work @default.
- W1975572249 sameAs 1975572249 @default.
- W1975572249 citedByCount "37" @default.
- W1975572249 countsByYear W19755722492014 @default.
- W1975572249 countsByYear W19755722492015 @default.
- W1975572249 countsByYear W19755722492018 @default.
- W1975572249 countsByYear W19755722492021 @default.
- W1975572249 crossrefType "journal-article" @default.
- W1975572249 hasAuthorship W1975572249A5009916043 @default.
- W1975572249 hasAuthorship W1975572249A5010336415 @default.
- W1975572249 hasAuthorship W1975572249A5029245450 @default.
- W1975572249 hasAuthorship W1975572249A5053778905 @default.
- W1975572249 hasAuthorship W1975572249A5078109225 @default.
- W1975572249 hasAuthorship W1975572249A5079606283 @default.
- W1975572249 hasAuthorship W1975572249A5081958388 @default.
- W1975572249 hasAuthorship W1975572249A5085337453 @default.
- W1975572249 hasBestOaLocation W19755722491 @default.
- W1975572249 hasConcept C104317684 @default.
- W1975572249 hasConcept C128319531 @default.
- W1975572249 hasConcept C151292725 @default.
- W1975572249 hasConcept C153911025 @default.
- W1975572249 hasConcept C167625842 @default.
- W1975572249 hasConcept C181199279 @default.
- W1975572249 hasConcept C2776295094 @default.
- W1975572249 hasConcept C2777598261 @default.
- W1975572249 hasConcept C515207424 @default.
- W1975572249 hasConcept C55493867 @default.
- W1975572249 hasConcept C86803240 @default.
- W1975572249 hasConcept C92825506 @default.
- W1975572249 hasConceptScore W1975572249C104317684 @default.
- W1975572249 hasConceptScore W1975572249C128319531 @default.
- W1975572249 hasConceptScore W1975572249C151292725 @default.
- W1975572249 hasConceptScore W1975572249C153911025 @default.
- W1975572249 hasConceptScore W1975572249C167625842 @default.
- W1975572249 hasConceptScore W1975572249C181199279 @default.
- W1975572249 hasConceptScore W1975572249C2776295094 @default.
- W1975572249 hasConceptScore W1975572249C2777598261 @default.
- W1975572249 hasConceptScore W1975572249C515207424 @default.
- W1975572249 hasConceptScore W1975572249C55493867 @default.
- W1975572249 hasConceptScore W1975572249C86803240 @default.
- W1975572249 hasConceptScore W1975572249C92825506 @default.
- W1975572249 hasIssue "2" @default.
- W1975572249 hasLocation W19755722491 @default.
- W1975572249 hasOpenAccess W1975572249 @default.
- W1975572249 hasPrimaryLocation W19755722491 @default.
- W1975572249 hasRelatedWork W1971686505 @default.
- W1975572249 hasRelatedWork W1975572249 @default.
- W1975572249 hasRelatedWork W1975989630 @default.
- W1975572249 hasRelatedWork W1990700206 @default.
- W1975572249 hasRelatedWork W2069709460 @default.
- W1975572249 hasRelatedWork W2088425074 @default.
- W1975572249 hasRelatedWork W2410954609 @default.
- W1975572249 hasRelatedWork W2413723606 @default.
- W1975572249 hasRelatedWork W2570740623 @default.
- W1975572249 hasRelatedWork W4230638069 @default.
- W1975572249 hasVolume "38" @default.
- W1975572249 isParatext "false" @default.
- W1975572249 isRetracted "false" @default.
- W1975572249 magId "1975572249" @default.
- W1975572249 workType "article" @default.