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- W1975629365 abstract "Promastigotes of Leishmania donovani that had been subcultued in modified Tobie's medium for 2 to 3 years showed decreased infectivity and lack of virulence for hamsters and mice compared to newly transformed promastigotes. Amastigotes derived from these long-term promastigote cultures decreased in number rapidly in hamsters, but only slightly in mice, over a 48-day period. In cultured mouse and hamster macrophags infected in vitro, amastigotes derived from long-term cultures rapidly decreased to low numbers, which persisted. The same pattern was seen in macriphages treated with catalase, an inhibitor of the oxygen-dependent killing mechanism of the macrophage. Promastigotes from long-term cultures also differed from virulent first-passage promastigotes in size, growth patterns in Tabie's medium, and in the quantities of some of their antigens." @default.
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- W1975629365 date "1985-02-01" @default.
- W1975629365 modified "2023-10-16" @default.
- W1975629365 title "Effects of Long-Term in Vitro Cultivation on Leishmania donovani Promastigotes" @default.
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- W1975629365 doi "https://doi.org/10.1111/j.1550-7408.1985.tb03015.x" @default.
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