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- W1975635148 abstract "Abstract We examined insulin formulations of soluble pork and human insulin with High Performance Liquid Chromatography (HPLC), using reversed phase and gel-filtration chromatography (column temperature:ambient temperature and 37[ddot]C). We were unable to find any impurities or aggregates of higher molecular weight, nor detect differences in the composition of the investigated insulins even ten months after production. The effects of temperature, salt concentration and dilution upon gel-filtration were also studied. After shaking at 37[ddot]C during 72 h, under exactly controlled conditions, some insulins showed a milky suspension. However, the immediately performed gel-filtration surprisingly showed in no case aggregates of higher molecular weight, but a reduction of the quantity of insulin. At ambient temperature the shaken insulin formulations remained clear. Here again no associations were detectable. To elucidate the nature of the sedimentation further investigations are necessary. But these investi..." @default.
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- W1975635148 date "1985-05-01" @default.
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- W1975635148 title "The Influence of Motion and Temperature upon the Aggregational Behavior of Soluble Insulin Formulations Investigated by High Performance Liquid Chromatography" @default.
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- W1975635148 doi "https://doi.org/10.1080/01483918508067133" @default.
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