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• W1975711819 endingPage "722" @default.
• W1975711819 startingPage "713" @default.
• W1975711819 abstract "We have checked the ability of the Candida albicans GAPDH polypeptide, which lacks a conventional N-terminal signal peptide, to reach the cell wall in Saccharomyces cerevisiae by using an intracellular form of the yeast invertase as a reporter protein. A hybrid TDH3-SUC2 gene containing the C. albicans TDH3 promoter sequences and a coding region encoding a fusion protein formed by the C. albicans GAPDH polypeptide, fused at its C-terminus with the yeast internal invertase, was constructed in a centromer derivative plasmid and transformed into a Suc(-) S. cerevisiae strain. Transformants displayed invertase activity measured in intact whole cells, and were able to grow on sucrose as the sole fermentable carbon source. Northern blot analysis with both TDH3 and SUC2 probes detected a single mRNA species of the expected size (about 2.7 kb), and Western immunoblot analysis of cell-free extracts, using a monoclonal antibody (mAb49) against a C. albicans GAPDH epitope, showed the presence of a 90 kDa polypeptide corresponding to the GAPDH-invertase fusion protein. This indicates that the TDH3 gene is able to direct part of the encoded gene product to the cell wall, and that any putative motifs for this targeting should be within the GAPDH amino acid sequence. Further analysis, using the same approach, of a panel of seven N- and C-terminal GAPDH truncates revealed that the region required for the cell wall targeting is located within the N-terminal half of the protein." @default.
• W1975711819 created "2016-06-24" @default.
• W1975711819 creator A5023064750 @default.
• W1975711819 creator A5049626267 @default.
• W1975711819 creator A5071209698 @default.
• W1975711819 date "2003-05-15" @default.
• W1975711819 modified "2023-10-09" @default.
• W1975711819 title "Candida albicans TDH3 gene promotes secretion of internal invertase when expressed inSaccharomyces cerevisiae as a glyceraldehyde-3-phosphate dehydrogenase-invertase fusion protein" @default.
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• W1975711819 doi "https://doi.org/10.1002/yea.993" @default.
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