FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1975759561> ?p ?o ?g. }

• W1975759561 endingPage "991" @default.
• W1975759561 startingPage "984" @default.
• W1975759561 abstract "Background The dust mite Lepidoglyphus destructor is a major cause of allergic diseases among farmers. We have previously cloned and sequenced two isoforms of the major allergen Lep d 2 (formerly designated Lep d 1) and found significant homology to group 2 allergens of the house dust mite species Dermatophagoides. We now report on the production and characterization of recombinant Lep d 2. Objective We have expressed both isoforms in two different expression systems; a eukaryotic system, baculovirus in insect cells and a prokaryotic system, E. coli. We have compared the two systems in regard to production yields and immunoreactivity of the recombinant allergens. Methods The complete cDNA including the natural leader sequence was cloned into the pBlueBacIII transfer vector, and the rLep d 2 was produced as a secreted protein in baculovirus. For the expression in E. coli, the cDNA was cloned into the pET vector, and the rLep d 2 was produced with six C-terminal histidine residues. The purified recombinant allergens were tested for immunoreactivity with 10 sera from subjects allergic to Lepidoglyphus destructor and were compared with native Lep d 2 using inhibition immunoblotting. The ability of the recombinant allergens to release histamine from basophils was evaluated using a histamine release assay. Results Both expression systems produced immunoreactive recombinant allergens. They inhibited the binding of human sera to native Lep d 2 confirming their retained IgE binding properties. The yield of pure recombinant protein from the prokaryotic system was ≈ 1 mg/L compared to the eukaryotic system which produced up to 4 mg/L in an adherent cell culture system. Conclusions We have produced recombinant Lep d 2 in prokaryotic and eukaryotic expression systems which are comparable to the native allergen. Recombinant Lep d 2 might now be included in more extensive clinical studies to confirm its usefulness in the in vitro and the in vivo diagnosis of Lepidoglyphus destructor." @default.
• W1975759561 created "2016-06-24" @default.
• W1975759561 creator A5009457161 @default.
• W1975759561 creator A5012491902 @default.
• W1975759561 creator A5018391465 @default.
• W1975759561 creator A5026409328 @default.
• W1975759561 creator A5063001647 @default.
• W1975759561 creator A5067270983 @default.
• W1975759561 creator A5079943716 @default.
• W1975759561 date "1998-08-01" @default.
• W1975759561 modified "2023-10-18" @default.
• W1975759561 title "Expression of two isoforms of Lep d 2, the major allergen of <i>Lepidoglyphus destructor</i> , in both prokaryotic and eukaryotic systems" @default.
• W1975759561 cites W1796450354 @default.
• W1975759561 cites W1967736264 @default.
• W1975759561 cites W1977233035 @default.
• W1975759561 cites W1982731765 @default.
• W1975759561 cites W2003902333 @default.
• W1975759561 cites W2005945834 @default.
• W1975759561 cites W2007086959 @default.
• W1975759561 cites W2009676329 @default.
• W1975759561 cites W2012244548 @default.
• W1975759561 cites W2012405469 @default.
• W1975759561 cites W2021848920 @default.
• W1975759561 cites W2022708664 @default.
• W1975759561 cites W2028690356 @default.
• W1975759561 cites W2034341349 @default.
• W1975759561 cites W2036723645 @default.
• W1975759561 cites W2036759647 @default.
• W1975759561 cites W2039988451 @default.
• W1975759561 cites W2053973011 @default.
• W1975759561 cites W2054962808 @default.
• W1975759561 cites W2062094705 @default.
• W1975759561 cites W2063655248 @default.
• W1975759561 cites W2064746815 @default.
• W1975759561 cites W2066203499 @default.
• W1975759561 cites W2093278468 @default.
• W1975759561 cites W2094879274 @default.
• W1975759561 cites W2152918358 @default.
• W1975759561 cites W2157462100 @default.
• W1975759561 cites W2160643203 @default.
• W1975759561 cites W2170293208 @default.
• W1975759561 doi "https://doi.org/10.1046/j.1365-2222.1998.00375.x" @default.
• W1975759561 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9756203" @default.
• W1975759561 hasPublicationYear "1998" @default.
• W1975759561 type Work @default.
• W1975759561 sameAs 1975759561 @default.
• W1975759561 citedByCount "34" @default.
• W1975759561 countsByYear W19757595612012 @default.
• W1975759561 countsByYear W19757595612013 @default.
• W1975759561 countsByYear W19757595612014 @default.
• W1975759561 countsByYear W19757595612018 @default.
• W1975759561 countsByYear W19757595612019 @default.
• W1975759561 crossrefType "journal-article" @default.
• W1975759561 hasAuthorship W1975759561A5009457161 @default.
• W1975759561 hasAuthorship W1975759561A5012491902 @default.
• W1975759561 hasAuthorship W1975759561A5018391465 @default.
• W1975759561 hasAuthorship W1975759561A5026409328 @default.
• W1975759561 hasAuthorship W1975759561A5063001647 @default.
• W1975759561 hasAuthorship W1975759561A5067270983 @default.
• W1975759561 hasAuthorship W1975759561A5079943716 @default.
• W1975759561 hasConcept C104317684 @default.
• W1975759561 hasConcept C1122143 @default.
• W1975759561 hasConcept C134018914 @default.
• W1975759561 hasConcept C141105273 @default.
• W1975759561 hasConcept C153911025 @default.
• W1975759561 hasConcept C159654299 @default.
• W1975759561 hasConcept C185592680 @default.
• W1975759561 hasConcept C187882448 @default.
• W1975759561 hasConcept C203014093 @default.
• W1975759561 hasConcept C207480886 @default.
• W1975759561 hasConcept C2778166374 @default.
• W1975759561 hasConcept C2780510475 @default.
• W1975759561 hasConcept C40767141 @default.
• W1975759561 hasConcept C53345823 @default.
• W1975759561 hasConcept C547475151 @default.
• W1975759561 hasConcept C55493867 @default.
• W1975759561 hasConcept C86803240 @default.
• W1975759561 hasConceptScore W1975759561C104317684 @default.
• W1975759561 hasConceptScore W1975759561C1122143 @default.
• W1975759561 hasConceptScore W1975759561C134018914 @default.
• W1975759561 hasConceptScore W1975759561C141105273 @default.
• W1975759561 hasConceptScore W1975759561C153911025 @default.
• W1975759561 hasConceptScore W1975759561C159654299 @default.
• W1975759561 hasConceptScore W1975759561C185592680 @default.
• W1975759561 hasConceptScore W1975759561C187882448 @default.
• W1975759561 hasConceptScore W1975759561C203014093 @default.
• W1975759561 hasConceptScore W1975759561C207480886 @default.
• W1975759561 hasConceptScore W1975759561C2778166374 @default.
• W1975759561 hasConceptScore W1975759561C2780510475 @default.
• W1975759561 hasConceptScore W1975759561C40767141 @default.
• W1975759561 hasConceptScore W1975759561C53345823 @default.
• W1975759561 hasConceptScore W1975759561C547475151 @default.
• W1975759561 hasConceptScore W1975759561C55493867 @default.
• W1975759561 hasConceptScore W1975759561C86803240 @default.
• W1975759561 hasIssue "8" @default.
• W1975759561 hasLocation W19757595611 @default.
• W1975759561 hasLocation W19757595612 @default.
• W1975759561 hasOpenAccess W1975759561 @default.

• next