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• W1975768442 abstract "Abstract Isothermal titration calorimetry was firstly applied to investigate the metal-binding properties of metallothionein. Thermodynamic data of the replacement of metallothionein (Zn7MT) and the reconstitution of thionein (apoMT) with Pb(II) ion at pH 4.70 have been examined. The UV and CD spectrometries were also used to identify the products. The product of the reaction of Pb2+ with Zn7MT (reaction 1), Pb7MT, has a similar structure with Zn7MT, while a new lead–MT complex, Pb7MT′, is formed in the case of reconstitution of apoMT (reaction 2). The thermodynamic parameters of the reactions for MT binding each mole of lead(II), ΔH1=−25.2 kJ mol−1, ΔG1=−28.7 kJ mol−1, K1=1.3×105, ΔS1=11.6 J mol−1 K−1 and ΔH2=−17.8 kJ mol−1, ΔG2=−32.4 kJ mol−1, K2=5.3×105, ΔS2=48.8 J mol−1 K−1, demonstrate that both reactions are spontaneous, exothermic, and entropy-increasing processes. The contributions of relative interactions in both reactions to enthalpy and entropy changes are discussed." @default.
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• W1975768442 date "2000-07-01" @default.
• W1975768442 modified "2023-09-26" @default.
• W1975768442 title "The microcalorimetry study on the complexation of lead ion with metallothionein" @default.
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• W1975768442 doi "https://doi.org/10.1016/s0040-6031(99)00468-2" @default.
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