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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1975813797> ?p ?o ?g. }

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• W1975813797 abstract "In cell biology, the study of proteins and organelles requires the combination of different imaging approaches, from live recordings with light microscopy (LM) to electron microscopy (EM).To correlate dynamic events in adherent cells with both ultrastructural and 3D information, we developed a method for cultured cells that combines confocal time-lapse images of GFP-tagged proteins with electron microscopy. With laser micro-patterned culture substrate, we created coordinates that were conserved at every step of the sample preparation and visualization processes. Specifically designed for cryo-fixation, this method allowed a fast freezing of dynamic events within seconds and their ultrastructural characterization. We provide examples of the dynamic oligomerization of GFP-tagged myotubularin (MTM1) phosphoinositides phosphatase induced by osmotic stress, and of the ultrastructure of membrane tubules dependent on amphiphysin 2 (BIN1) expression.Accessible and versatile, we show that this approach is efficient to routinely correlate functional and dynamic LM with high resolution morphology by EM, with immuno-EM labeling, with 3D reconstruction using serial immuno-EM or tomography, and with scanning-EM." @default.
• W1975813797 created "2016-06-24" @default.
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• W1975813797 date "2010-02-03" @default.
• W1975813797 modified "2023-10-16" @default.
• W1975813797 title "From Dynamic Live Cell Imaging to 3D Ultrastructure: Novel Integrated Methods for High Pressure Freezing and Correlative Light-Electron Microscopy" @default.
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• W1975813797 doi "https://doi.org/10.1371/journal.pone.0009014" @default.
• W1975813797 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2815783" @default.
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