FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1975829294> ?p ?o ?g. }

• W1975829294 endingPage "279" @default.
• W1975829294 startingPage "271" @default.
• W1975829294 abstract "Procedures for the isolation and enrichment of cell populations from suspensions of rat kidney cortical cells were developed. Using Percoll density-gradient centrifugation, two populations of cells were obtained; marker enzymes [alkaline phosphatase and gamma-glutamyltransferase for proximal tubular (PT) cells and hexokinase for distal tubular (DT) cells] and functional responses (stimulation of PT cell oxygen consumption by succinate and inhibition of DT cell oxygen consumption by amiloride) were then employed to identify and assess the purity of the two fractions. The PT cell fraction was estimated to contain 97% PT cells and the DT cell fraction was estimated to contain 88% DT cells. Staining with toluidine blue and light microscopy showed that PT cells contained a brush border, were larger than DT cells, and had more intensely staining nuclei than DT cells. To demonstrate the usefulness of these cell preparations in the study of biochemical mechanisms of renal cell injury, time- and concentration-dependent effects of the PT cell-specific nephrotoxin cephaloridine (CPH) on PT and DT cell trypan blue exclusion were examined. CPH was toxic in PT cells but not in DT cells; viability of PT cells incubated with 0.1 or 1 mM CPH for 2 h was 57 or 34%, respectively, compared to 81% for control cells; viability of DT cells incubated with 0.1 or 1 mM CPH for 2 h was 74 or 71%, respectively, compared to 74% for control cells. This method thus provides highly enriched preparations of freshly isolated PT and DT cells that retain their unique properties and are suitable for studies of biochemical mechanisms of chemical toxicity and nephron heterogeneity." @default.
• W1975829294 created "2016-06-24" @default.
• W1975829294 creator A5010943334 @default.
• W1975829294 creator A5030738786 @default.
• W1975829294 date "1989-11-01" @default.
• W1975829294 modified "2023-09-23" @default.
• W1975829294 title "Isolation of two distinct populations of cells from fat kidney cortex and their use in the study of chemical-induced toxicity" @default.
• W1975829294 cites W135258530 @default.
• W1975829294 cites W1528261036 @default.
• W1975829294 cites W1540384225 @default.
• W1975829294 cites W1566730133 @default.
• W1975829294 cites W1568987190 @default.
• W1975829294 cites W1775749144 @default.
• W1975829294 cites W1976590152 @default.
• W1975829294 cites W1979315000 @default.
• W1975829294 cites W1985981766 @default.
• W1975829294 cites W2021505696 @default.
• W1975829294 cites W2041976890 @default.
• W1975829294 cites W2044803485 @default.
• W1975829294 cites W2047689493 @default.
• W1975829294 cites W2057710551 @default.
• W1975829294 cites W2058979221 @default.
• W1975829294 cites W2070486792 @default.
• W1975829294 cites W2076737325 @default.
• W1975829294 cites W2086229992 @default.
• W1975829294 cites W2091695540 @default.
• W1975829294 cites W2093992101 @default.
• W1975829294 cites W2118519973 @default.
• W1975829294 cites W2332574843 @default.
• W1975829294 cites W2338905722 @default.
• W1975829294 cites W4241950997 @default.
• W1975829294 doi "https://doi.org/10.1016/0003-2697(89)90593-9" @default.
• W1975829294 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2692474" @default.
• W1975829294 hasPublicationYear "1989" @default.
• W1975829294 type Work @default.
• W1975829294 sameAs 1975829294 @default.
• W1975829294 citedByCount "67" @default.
• W1975829294 countsByYear W19758292942012 @default.
• W1975829294 countsByYear W19758292942013 @default.
• W1975829294 countsByYear W19758292942015 @default.
• W1975829294 countsByYear W19758292942016 @default.
• W1975829294 countsByYear W19758292942021 @default.
• W1975829294 crossrefType "journal-article" @default.
• W1975829294 hasAuthorship W1975829294A5010943334 @default.
• W1975829294 hasAuthorship W1975829294A5030738786 @default.
• W1975829294 hasConcept C132946820 @default.
• W1975829294 hasConcept C1491633281 @default.
• W1975829294 hasConcept C153911025 @default.
• W1975829294 hasConcept C160160445 @default.
• W1975829294 hasConcept C181199279 @default.
• W1975829294 hasConcept C185592680 @default.
• W1975829294 hasConcept C2583947 @default.
• W1975829294 hasConcept C2780070996 @default.
• W1975829294 hasConcept C29537977 @default.
• W1975829294 hasConcept C48679268 @default.
• W1975829294 hasConcept C53227056 @default.
• W1975829294 hasConcept C54355233 @default.
• W1975829294 hasConcept C55493867 @default.
• W1975829294 hasConcept C74864618 @default.
• W1975829294 hasConcept C7623868 @default.
• W1975829294 hasConcept C81885089 @default.
• W1975829294 hasConcept C86803240 @default.
• W1975829294 hasConceptScore W1975829294C132946820 @default.
• W1975829294 hasConceptScore W1975829294C1491633281 @default.
• W1975829294 hasConceptScore W1975829294C153911025 @default.
• W1975829294 hasConceptScore W1975829294C160160445 @default.
• W1975829294 hasConceptScore W1975829294C181199279 @default.
• W1975829294 hasConceptScore W1975829294C185592680 @default.
• W1975829294 hasConceptScore W1975829294C2583947 @default.
• W1975829294 hasConceptScore W1975829294C2780070996 @default.
• W1975829294 hasConceptScore W1975829294C29537977 @default.
• W1975829294 hasConceptScore W1975829294C48679268 @default.
• W1975829294 hasConceptScore W1975829294C53227056 @default.
• W1975829294 hasConceptScore W1975829294C54355233 @default.
• W1975829294 hasConceptScore W1975829294C55493867 @default.
• W1975829294 hasConceptScore W1975829294C74864618 @default.
• W1975829294 hasConceptScore W1975829294C7623868 @default.
• W1975829294 hasConceptScore W1975829294C81885089 @default.
• W1975829294 hasConceptScore W1975829294C86803240 @default.
• W1975829294 hasIssue "2" @default.
• W1975829294 hasLocation W19758292941 @default.
• W1975829294 hasLocation W19758292942 @default.
• W1975829294 hasOpenAccess W1975829294 @default.
• W1975829294 hasPrimaryLocation W19758292941 @default.
• W1975829294 hasRelatedWork W1975829294 @default.
• W1975829294 hasRelatedWork W1985384359 @default.
• W1975829294 hasRelatedWork W1990284973 @default.
• W1975829294 hasRelatedWork W2042312824 @default.
• W1975829294 hasRelatedWork W2078247653 @default.
• W1975829294 hasRelatedWork W2086827828 @default.
• W1975829294 hasRelatedWork W2088668395 @default.
• W1975829294 hasRelatedWork W2366786834 @default.
• W1975829294 hasRelatedWork W2388215032 @default.
• W1975829294 hasRelatedWork W2914449720 @default.
• W1975829294 hasVolume "182" @default.
• W1975829294 isParatext "false" @default.
• W1975829294 isRetracted "false" @default.
• W1975829294 magId "1975829294" @default.

• next