Matches in SemOpenAlex for { <https://semopenalex.org/work/W1975830291> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W1975830291 endingPage "623" @default.
- W1975830291 startingPage "619" @default.
- W1975830291 abstract "S-acetylthio-2-methylpropionic acid (S-AMPA) is an important chiral intermediary for numerous hypertension drugs such as captopril. S-AMPA can be produced by hydrolyzing the corresponding racemic methyl MAMP (S,R-methyl-β-acetylthioisobutyrate) by lipases or esterases that have the appropriate stereo specificity. Psudomonas fluorescens IFO 12055 possessing a highly specific lipase was used to process this reaction in the form of immobilized cells. Reaction kinetic and immobilization methods were also studied. Strong product inhibition was observed, that is, at 3% S-AMPA (namely 183 mM), activity was reduced by 50%. Spontaneous hydrolysis of the ester and thioester bonds was also observed, and was independent of the cells. Thus, reaction selectivity and yield must be optimized through adjusting the substrate concentration and total biocatalyst activity. Conventional calcium alginate (3% w/w) encapsulation was modified by adding 3% w/w polyethyleneimine (PEI) and cross-linked by a biologically derived agent, genipin (5.6 mM). This method was found to be satisfactory to produce stable and functioning biocatalyst and can maintain high reactivity for repeated 25 batches with e.e. values above 90%." @default.
- W1975830291 created "2016-06-24" @default.
- W1975830291 creator A5024245814 @default.
- W1975830291 creator A5067200824 @default.
- W1975830291 date "2004-12-01" @default.
- W1975830291 modified "2023-10-12" @default.
- W1975830291 title "Immobilized cells biocatalyst for the production of S-acetylthio-2-methyl propionic acid" @default.
- W1975830291 cites W1598050093 @default.
- W1975830291 cites W1967320422 @default.
- W1975830291 cites W1967568703 @default.
- W1975830291 cites W1968494270 @default.
- W1975830291 cites W1974160158 @default.
- W1975830291 cites W1981946908 @default.
- W1975830291 cites W1984795021 @default.
- W1975830291 cites W2012811667 @default.
- W1975830291 cites W2051260617 @default.
- W1975830291 cites W2067763402 @default.
- W1975830291 cites W2069911221 @default.
- W1975830291 cites W2079173351 @default.
- W1975830291 cites W2084468911 @default.
- W1975830291 cites W2122759189 @default.
- W1975830291 doi "https://doi.org/10.1016/j.enzmictec.2004.08.015" @default.
- W1975830291 hasPublicationYear "2004" @default.
- W1975830291 type Work @default.
- W1975830291 sameAs 1975830291 @default.
- W1975830291 citedByCount "5" @default.
- W1975830291 countsByYear W19758302912014 @default.
- W1975830291 countsByYear W19758302912015 @default.
- W1975830291 countsByYear W19758302912019 @default.
- W1975830291 crossrefType "journal-article" @default.
- W1975830291 hasAuthorship W1975830291A5024245814 @default.
- W1975830291 hasAuthorship W1975830291A5067200824 @default.
- W1975830291 hasConcept C134121241 @default.
- W1975830291 hasConcept C161790260 @default.
- W1975830291 hasConcept C178790620 @default.
- W1975830291 hasConcept C181199279 @default.
- W1975830291 hasConcept C185592680 @default.
- W1975830291 hasConcept C191503008 @default.
- W1975830291 hasConcept C191897082 @default.
- W1975830291 hasConcept C192562407 @default.
- W1975830291 hasConcept C2778713300 @default.
- W1975830291 hasConcept C2779697368 @default.
- W1975830291 hasConcept C519063684 @default.
- W1975830291 hasConcept C65024703 @default.
- W1975830291 hasConcept C71240020 @default.
- W1975830291 hasConcept C94412978 @default.
- W1975830291 hasConceptScore W1975830291C134121241 @default.
- W1975830291 hasConceptScore W1975830291C161790260 @default.
- W1975830291 hasConceptScore W1975830291C178790620 @default.
- W1975830291 hasConceptScore W1975830291C181199279 @default.
- W1975830291 hasConceptScore W1975830291C185592680 @default.
- W1975830291 hasConceptScore W1975830291C191503008 @default.
- W1975830291 hasConceptScore W1975830291C191897082 @default.
- W1975830291 hasConceptScore W1975830291C192562407 @default.
- W1975830291 hasConceptScore W1975830291C2778713300 @default.
- W1975830291 hasConceptScore W1975830291C2779697368 @default.
- W1975830291 hasConceptScore W1975830291C519063684 @default.
- W1975830291 hasConceptScore W1975830291C65024703 @default.
- W1975830291 hasConceptScore W1975830291C71240020 @default.
- W1975830291 hasConceptScore W1975830291C94412978 @default.
- W1975830291 hasIssue "6-7" @default.
- W1975830291 hasLocation W19758302911 @default.
- W1975830291 hasOpenAccess W1975830291 @default.
- W1975830291 hasPrimaryLocation W19758302911 @default.
- W1975830291 hasRelatedWork W1551032865 @default.
- W1975830291 hasRelatedWork W2055589734 @default.
- W1975830291 hasRelatedWork W2094752049 @default.
- W1975830291 hasRelatedWork W2223493887 @default.
- W1975830291 hasRelatedWork W2246782474 @default.
- W1975830291 hasRelatedWork W2355386734 @default.
- W1975830291 hasRelatedWork W2358749095 @default.
- W1975830291 hasRelatedWork W2359434416 @default.
- W1975830291 hasRelatedWork W238889935 @default.
- W1975830291 hasRelatedWork W2510923768 @default.
- W1975830291 hasVolume "35" @default.
- W1975830291 isParatext "false" @default.
- W1975830291 isRetracted "false" @default.
- W1975830291 magId "1975830291" @default.
- W1975830291 workType "article" @default.