Matches in SemOpenAlex for { <https://semopenalex.org/work/W1975915667> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W1975915667 endingPage "159" @default.
- W1975915667 startingPage "152" @default.
- W1975915667 abstract "Two isozymes of β - N -acetylglucosaminidase (2-acetamido-2-deoxy-β- d -glucoside acetamidodeoxy glucohydrolase, EC 3.2.1.30) (A and B) from bull seminal plasma were purified to homogeneity by isoelectric focusing having p I values of 5.31 and 6.78. The two proteins were glycoproteins with very similar amino acid composition but isozyme A contained more sialic acid than isozyme B. The molecular weights of isozyme A and B were estimated at 200 000 and 190 000 by gel filtration. Two identical subunits corresponding to molecular weights of 53 000 and 13 400 were obtained from hexosaminidase A and B when subjected to polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate. Similar results were obtained when dissociation of the isozymes was effected with mercaptoethanol, guanidine hydrochloride and urea in presence of sodium dodecyl sulphate and the subunits separated by acrylamide gel electrophoresis. The two isozymes were more stable in frozen conditions than at the refrigerated temperature. Of the divalent ion tested, glucosaminidase and galactosaminidase activities of isozymes A and B were strongly inhibited by Hg 2+ and Ag + thus suggesting the presence of thiol groups in the two proteins. The two isozymes were active on natural substrates; isozyme B being more active than isozyme A." @default.
- W1975915667 created "2016-06-24" @default.
- W1975915667 creator A5069107396 @default.
- W1975915667 creator A5071496681 @default.
- W1975915667 date "1977-07-01" @default.
- W1975915667 modified "2023-10-17" @default.
- W1975915667 title "Studies on the glycosidases of semen. Further purification and characterization of two hexosaminidases from bull seminal plasma" @default.
- W1975915667 cites W1535002104 @default.
- W1975915667 cites W1540136730 @default.
- W1975915667 cites W1546479429 @default.
- W1975915667 cites W1580023435 @default.
- W1975915667 cites W1775749144 @default.
- W1975915667 cites W1807830428 @default.
- W1975915667 cites W1810642313 @default.
- W1975915667 cites W1908408342 @default.
- W1975915667 cites W2034621532 @default.
- W1975915667 cites W2052332023 @default.
- W1975915667 cites W2058345486 @default.
- W1975915667 cites W2064753822 @default.
- W1975915667 cites W2073879684 @default.
- W1975915667 cites W2395079662 @default.
- W1975915667 cites W2413459049 @default.
- W1975915667 doi "https://doi.org/10.1016/0005-2744(77)90017-1" @default.
- W1975915667 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/880304" @default.
- W1975915667 hasPublicationYear "1977" @default.
- W1975915667 type Work @default.
- W1975915667 sameAs 1975915667 @default.
- W1975915667 citedByCount "9" @default.
- W1975915667 crossrefType "journal-article" @default.
- W1975915667 hasAuthorship W1975915667A5069107396 @default.
- W1975915667 hasAuthorship W1975915667A5071496681 @default.
- W1975915667 hasConcept C101660505 @default.
- W1975915667 hasConcept C116084860 @default.
- W1975915667 hasConcept C119795356 @default.
- W1975915667 hasConcept C178790620 @default.
- W1975915667 hasConcept C181199279 @default.
- W1975915667 hasConcept C185592680 @default.
- W1975915667 hasConcept C193363816 @default.
- W1975915667 hasConcept C2780115458 @default.
- W1975915667 hasConcept C2780365088 @default.
- W1975915667 hasConcept C40250595 @default.
- W1975915667 hasConcept C43617362 @default.
- W1975915667 hasConcept C50476208 @default.
- W1975915667 hasConcept C537181965 @default.
- W1975915667 hasConcept C55493867 @default.
- W1975915667 hasConcept C58121356 @default.
- W1975915667 hasConcept C74884574 @default.
- W1975915667 hasConcept C96294017 @default.
- W1975915667 hasConceptScore W1975915667C101660505 @default.
- W1975915667 hasConceptScore W1975915667C116084860 @default.
- W1975915667 hasConceptScore W1975915667C119795356 @default.
- W1975915667 hasConceptScore W1975915667C178790620 @default.
- W1975915667 hasConceptScore W1975915667C181199279 @default.
- W1975915667 hasConceptScore W1975915667C185592680 @default.
- W1975915667 hasConceptScore W1975915667C193363816 @default.
- W1975915667 hasConceptScore W1975915667C2780115458 @default.
- W1975915667 hasConceptScore W1975915667C2780365088 @default.
- W1975915667 hasConceptScore W1975915667C40250595 @default.
- W1975915667 hasConceptScore W1975915667C43617362 @default.
- W1975915667 hasConceptScore W1975915667C50476208 @default.
- W1975915667 hasConceptScore W1975915667C537181965 @default.
- W1975915667 hasConceptScore W1975915667C55493867 @default.
- W1975915667 hasConceptScore W1975915667C58121356 @default.
- W1975915667 hasConceptScore W1975915667C74884574 @default.
- W1975915667 hasConceptScore W1975915667C96294017 @default.
- W1975915667 hasIssue "1" @default.
- W1975915667 hasLocation W19759156671 @default.
- W1975915667 hasLocation W19759156672 @default.
- W1975915667 hasOpenAccess W1975915667 @default.
- W1975915667 hasPrimaryLocation W19759156671 @default.
- W1975915667 hasRelatedWork W1515746114 @default.
- W1975915667 hasRelatedWork W1648512565 @default.
- W1975915667 hasRelatedWork W1966474501 @default.
- W1975915667 hasRelatedWork W1987262605 @default.
- W1975915667 hasRelatedWork W2019405391 @default.
- W1975915667 hasRelatedWork W2033749400 @default.
- W1975915667 hasRelatedWork W2037968798 @default.
- W1975915667 hasRelatedWork W2078366744 @default.
- W1975915667 hasRelatedWork W2117141996 @default.
- W1975915667 hasRelatedWork W2374836858 @default.
- W1975915667 hasVolume "483" @default.
- W1975915667 isParatext "false" @default.
- W1975915667 isRetracted "false" @default.
- W1975915667 magId "1975915667" @default.
- W1975915667 workType "article" @default.