Matches in SemOpenAlex for { <https://semopenalex.org/work/W1975922979> ?p ?o ?g. }
- W1975922979 endingPage "4811" @default.
- W1975922979 startingPage "4801" @default.
- W1975922979 abstract "Hepatitis C virus (HCV) causes a persistent infection, chronic hepatitis and hepatocellular carcinoma. HCV NS5A, one of non-structural proteins of HCV, was suggested to play a role in oncogenic transformation. Since the tumor suppressor p53 is important for preventing neoplastic transformation, we investigated the functional effects of HCV NS5A on p53. In vitro and in vivo coimmunoprecipitation and confocal microscopy were used to determine the interaction of NS5A and p53. HCV NS5A binds directly to p53 and colocalizes p53 in the perinuclear region. NS5A inhibits transcriptional transactivation by p53 in a dose-dependent manner by use of a reporter assay. Down regulation of endogenous p21/waf1 expression, which is activated by p53 in Hep3B cells, by NS5A was demonstrated by using FLAG- and FLAG-NS5A Hep3B stable cell lines. The effect of NS5A on p53-mediated apoptosis was determined by flow cytometry in both NS5A permanently and transiently transfected Hep3B cells with exogenous p53. The p53-induced apoptosis was abrogated by NS5A and the inhibition effect correlates well with the binding ability of NS5A to p53. In addition, HCV NS5A protein interacts with and colocalizes hTAFII32, a component of TFIID and an essential coactivator of p53, in vivo. These results suggest that HCV NS5A interacts with and partially sequestrates p53 and hTAFII32 in the cytoplasm and suppresses p53-mediated transcriptional transactivation and apoptosis during HCV infection, which may contribute to the hepatocarcinogenesis of HCV infection." @default.
- W1975922979 created "2016-06-24" @default.
- W1975922979 creator A5017159595 @default.
- W1975922979 creator A5029486220 @default.
- W1975922979 creator A5031585842 @default.
- W1975922979 creator A5034244372 @default.
- W1975922979 creator A5034260893 @default.
- W1975922979 creator A5038786755 @default.
- W1975922979 creator A5041139736 @default.
- W1975922979 creator A5047674805 @default.
- W1975922979 creator A5054235677 @default.
- W1975922979 creator A5064580973 @default.
- W1975922979 creator A5077269939 @default.
- W1975922979 date "2002-07-09" @default.
- W1975922979 modified "2023-10-17" @default.
- W1975922979 title "HCV NS5A interacts with p53 and inhibits p53-mediated apoptosis" @default.
- W1975922979 cites W1838860459 @default.
- W1975922979 cites W1952751041 @default.
- W1975922979 cites W1971792395 @default.
- W1975922979 cites W1973165481 @default.
- W1975922979 cites W1978834861 @default.
- W1975922979 cites W1979319210 @default.
- W1975922979 cites W1984058368 @default.
- W1975922979 cites W1987536971 @default.
- W1975922979 cites W1988045726 @default.
- W1975922979 cites W1989636817 @default.
- W1975922979 cites W1991494214 @default.
- W1975922979 cites W1991865867 @default.
- W1975922979 cites W1991917274 @default.
- W1975922979 cites W2001030705 @default.
- W1975922979 cites W2012053348 @default.
- W1975922979 cites W2013468140 @default.
- W1975922979 cites W2018385269 @default.
- W1975922979 cites W2020484813 @default.
- W1975922979 cites W2021326683 @default.
- W1975922979 cites W2021825316 @default.
- W1975922979 cites W2028143792 @default.
- W1975922979 cites W2032451726 @default.
- W1975922979 cites W2033649274 @default.
- W1975922979 cites W2034296773 @default.
- W1975922979 cites W2035945932 @default.
- W1975922979 cites W2038500217 @default.
- W1975922979 cites W2048162805 @default.
- W1975922979 cites W2052014046 @default.
- W1975922979 cites W2053700118 @default.
- W1975922979 cites W2055349083 @default.
- W1975922979 cites W2064194464 @default.
- W1975922979 cites W2064947652 @default.
- W1975922979 cites W2066124462 @default.
- W1975922979 cites W2072763798 @default.
- W1975922979 cites W2073675795 @default.
- W1975922979 cites W2076685716 @default.
- W1975922979 cites W2078581194 @default.
- W1975922979 cites W2079395665 @default.
- W1975922979 cites W2080633751 @default.
- W1975922979 cites W2081763165 @default.
- W1975922979 cites W2081894694 @default.
- W1975922979 cites W2084725999 @default.
- W1975922979 cites W2094477759 @default.
- W1975922979 cites W2106836102 @default.
- W1975922979 cites W2107983204 @default.
- W1975922979 cites W2120483508 @default.
- W1975922979 cites W2122088474 @default.
- W1975922979 cites W2122151579 @default.
- W1975922979 cites W2124528668 @default.
- W1975922979 cites W2124761766 @default.
- W1975922979 cites W2129905922 @default.
- W1975922979 cites W2135909641 @default.
- W1975922979 cites W2150710841 @default.
- W1975922979 cites W2151898297 @default.
- W1975922979 cites W2153566433 @default.
- W1975922979 cites W2160306227 @default.
- W1975922979 cites W2160425277 @default.
- W1975922979 cites W2163565167 @default.
- W1975922979 cites W2168506744 @default.
- W1975922979 cites W2169499958 @default.
- W1975922979 cites W2328093310 @default.
- W1975922979 cites W2405173083 @default.
- W1975922979 cites W4206608510 @default.
- W1975922979 doi "https://doi.org/10.1038/sj.onc.1205589" @default.
- W1975922979 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12101418" @default.
- W1975922979 hasPublicationYear "2002" @default.
- W1975922979 type Work @default.
- W1975922979 sameAs 1975922979 @default.
- W1975922979 citedByCount "210" @default.
- W1975922979 countsByYear W19759229792012 @default.
- W1975922979 countsByYear W19759229792013 @default.
- W1975922979 countsByYear W19759229792014 @default.
- W1975922979 countsByYear W19759229792015 @default.
- W1975922979 countsByYear W19759229792016 @default.
- W1975922979 countsByYear W19759229792017 @default.
- W1975922979 countsByYear W19759229792018 @default.
- W1975922979 countsByYear W19759229792019 @default.
- W1975922979 countsByYear W19759229792020 @default.
- W1975922979 countsByYear W19759229792021 @default.
- W1975922979 countsByYear W19759229792022 @default.
- W1975922979 countsByYear W19759229792023 @default.
- W1975922979 crossrefType "journal-article" @default.