FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1975969445> ?p ?o ?g. }

• W1975969445 endingPage "e1001542" @default.
• W1975969445 startingPage "e1001542" @default.
• W1975969445 abstract "Secretory vesicles in endocrine cells store hormones such as growth hormone (GH) and insulin before their release into the bloodstream. The molecular mechanisms governing budding of immature secretory vesicles from the trans-Golgi network (TGN) and their subsequent maturation remain unclear. Here, we identify the lipid binding BAR (Bin/amphiphysin/Rvs) domain protein PICK1 (protein interacting with C kinase 1) as a key component early in the biogenesis of secretory vesicles in GH-producing cells. Both PICK1-deficient Drosophila and mice displayed somatic growth retardation. Growth retardation was rescued in flies by reintroducing PICK1 in neurosecretory cells producing somatotropic peptides. PICK1-deficient mice were characterized by decreased body weight and length, increased fat accumulation, impaired GH secretion, and decreased storage of GH in the pituitary. Decreased GH storage was supported by electron microscopy showing prominent reduction in secretory vesicle number. Evidence was also obtained for impaired insulin secretion associated with decreased glucose tolerance. PICK1 localized in cells to immature secretory vesicles, and the PICK1 BAR domain was shown by live imaging to associate with vesicles budding from the TGN and to possess membrane-sculpting properties in vitro. In mouse pituitary, PICK1 co-localized with the BAR domain protein ICA69, and PICK1 deficiency abolished ICA69 protein expression. In the Drosophila brain, PICK1 and ICA69 co-immunoprecipitated and showed mutually dependent expression. Finally, both in a Drosophila model of type 2 diabetes and in high-fat-diet-induced obese mice, we observed up-regulation of PICK1 mRNA expression. Our findings suggest that PICK1, together with ICA69, is critical during budding of immature secretory vesicles from the TGN and thus for vesicular storage of GH and possibly other hormones. The data link two BAR domain proteins to membrane remodeling processes in the secretory pathway of peptidergic endocrine cells and support an important role of PICK1/ICA69 in maintenance of metabolic homeostasis." @default.
• W1975969445 created "2016-06-24" @default.
• W1975969445 creator A5001961466 @default.
• W1975969445 creator A5011163375 @default.
• W1975969445 creator A5014924303 @default.
• W1975969445 creator A5020264771 @default.
• W1975969445 creator A5057521834 @default.
• W1975969445 creator A5058359207 @default.
• W1975969445 creator A5059333796 @default.
• W1975969445 creator A5060850281 @default.
• W1975969445 creator A5061554153 @default.
• W1975969445 creator A5065713713 @default.
• W1975969445 creator A5070305954 @default.
• W1975969445 creator A5070364192 @default.
• W1975969445 creator A5070435563 @default.
• W1975969445 creator A5071960807 @default.
• W1975969445 creator A5077648266 @default.
• W1975969445 creator A5079432233 @default.
• W1975969445 creator A5085804837 @default.
• W1975969445 creator A5090185420 @default.
• W1975969445 date "2013-04-23" @default.
• W1975969445 modified "2023-10-11" @default.
• W1975969445 title "PICK1 Deficiency Impairs Secretory Vesicle Biogenesis and Leads to Growth Retardation and Decreased Glucose Tolerance" @default.
• W1975969445 cites W1513490811 @default.
• W1975969445 cites W1601012886 @default.
• W1975969445 cites W1963923544 @default.
• W1975969445 cites W1973827770 @default.
• W1975969445 cites W1987835269 @default.
• W1975969445 cites W1987942547 @default.
• W1975969445 cites W1988097101 @default.
• W1975969445 cites W1996679823 @default.
• W1975969445 cites W2021431752 @default.
• W1975969445 cites W2021462093 @default.
• W1975969445 cites W2023863818 @default.
• W1975969445 cites W2025447674 @default.
• W1975969445 cites W2026896701 @default.
• W1975969445 cites W2029340539 @default.
• W1975969445 cites W2031976805 @default.
• W1975969445 cites W2035120267 @default.
• W1975969445 cites W2037936859 @default.
• W1975969445 cites W2041447616 @default.
• W1975969445 cites W2041848251 @default.
• W1975969445 cites W2049315117 @default.
• W1975969445 cites W2051178873 @default.
• W1975969445 cites W2051285749 @default.
• W1975969445 cites W2052542184 @default.
• W1975969445 cites W2054566118 @default.
• W1975969445 cites W2055367681 @default.
• W1975969445 cites W2058812127 @default.
• W1975969445 cites W2061270576 @default.
• W1975969445 cites W2062206747 @default.
• W1975969445 cites W2067090328 @default.
• W1975969445 cites W2069225280 @default.
• W1975969445 cites W2072634710 @default.
• W1975969445 cites W2076431574 @default.
• W1975969445 cites W2078591378 @default.
• W1975969445 cites W2079502881 @default.
• W1975969445 cites W2090663991 @default.
• W1975969445 cites W2098214132 @default.
• W1975969445 cites W2100344731 @default.
• W1975969445 cites W2100766258 @default.
• W1975969445 cites W2101544694 @default.
• W1975969445 cites W2106297617 @default.
• W1975969445 cites W2107028723 @default.
• W1975969445 cites W2108586733 @default.
• W1975969445 cites W2110643873 @default.
• W1975969445 cites W2115771723 @default.
• W1975969445 cites W2118067527 @default.
• W1975969445 cites W2121161073 @default.
• W1975969445 cites W2124336651 @default.
• W1975969445 cites W2124634396 @default.
• W1975969445 cites W2124733101 @default.
• W1975969445 cites W2131089409 @default.
• W1975969445 cites W2131424500 @default.
• W1975969445 cites W2134196276 @default.
• W1975969445 cites W2144489715 @default.
• W1975969445 cites W2149388099 @default.
• W1975969445 cites W2151390490 @default.
• W1975969445 cites W2153258878 @default.
• W1975969445 cites W2153551557 @default.
• W1975969445 cites W2154742909 @default.
• W1975969445 cites W2155783580 @default.
• W1975969445 cites W2160452185 @default.
• W1975969445 cites W2161239116 @default.
• W1975969445 cites W2167644932 @default.
• W1975969445 cites W2170590086 @default.
• W1975969445 cites W4236663041 @default.
• W1975969445 cites W4367662404 @default.
• W1975969445 cites W2611588052 @default.
• W1975969445 doi "https://doi.org/10.1371/journal.pbio.1001542" @default.
• W1975969445 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3635866" @default.
• W1975969445 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23630454" @default.
• W1975969445 hasPublicationYear "2013" @default.
• W1975969445 type Work @default.
• W1975969445 sameAs 1975969445 @default.
• W1975969445 citedByCount "72" @default.
• W1975969445 countsByYear W19759694452013 @default.
• W1975969445 countsByYear W19759694452014 @default.

• next