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- W1975970977 abstract "Image-based analysis of the vascular structures of murine liver samples is an important tool for scientists to understand liver physiology and morphology. Typical assessment methods are MicroCT, which allows for acquiringimages of the whole organ while lacking resolution for fine details, and confocal laser scanning microscopy, whichallows detailed insights into fine structures while lacking the broader context. Imaging of histological serial whole slide sections is a recent technology able to fill this gap, since it provides a fine resolution up to the cellular level, but on a whole organ scale. However, whole slide imaging is a modality providing only 2D images. Therefore the challenge is to use stacks of serial sections from which to reconstruct the 3D vessel structures. In this paper we present a semi-automatic procedure to achieve this goal. We employ an automatic method that detects vessel structures based on continuity and shape characteristics. Furthermore it supports the user to perform manual corrections where required. With our methods we were able to successfully extract and reconstruct vessel structures from a stack of 100 and a stack of 397 serial sections of a mouse liver lobe, thus proving the potential of our approach." @default.
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- W1975970977 date "2013-03-29" @default.
- W1975970977 modified "2023-09-23" @default.
- W1975970977 title "Reconstruction of vessel structures from serial whole slide sections of murine liver samples" @default.
- W1975970977 doi "https://doi.org/10.1117/12.2008112" @default.
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