FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1976013973> ?p ?o ?g. }

• W1976013973 endingPage "77" @default.
• W1976013973 startingPage "69" @default.
• W1976013973 abstract "The recombinant isolates of tobacco veinal necrotic strain of Potato virus Y (PVYN) and potato tuber necrotic group (PVY(NTN)) contain segments of the PVYO and the PVY(N) genome. Three major recombinant junctions (RJ) are present in the genome of the recombinant PVY(NTN) at sites HC/Pro-P3, 6K2-NIa, and the C-terminal region of CP gene and one RJ at HC/Pro-P3 site in some recombinant PVYN isolates (termed PVY(N:O)). Protocols for specific differentiation of the recombinant PVY(NTN) and PVY(N:O) from the non-recombinant PVYN are described. Specific primer pairs were designed to target the three RJs so that sense and antisense primers completely matched the nucleotide sequences at either side of the RJ. In a uniplex reverse transcription-polymerase chain reaction (RT-PCR), the first primer pair amplified a fragment of 641bp from the recombinant PVY(NTN) and PVY(N:O). The second and third primer pairs exclusively amplified fragments of 448 and 290bp, respectively from the recombinant PVY(NTN). In a multiplex (triplex) RT-PCR, when all three primer pairs were used simultaneously, the three fragments (641, 448 and 290bp) were amplified exclusively from the recombinant PVY(NTN), while only one fragment (641bp) was amplified from the PVY(N:O) isolates, clearly differentiating the two recombinant isolates. No amplification was observed from the non-recombinant PVY, including PVYO and North American (NA)-PVY(N/NTN). For further improvement of the multiplex RT-PCR, effects of cDNA preparation using specific antisense primers, random primers or oligo(dT) plus random primers were investigated. The cDNA prepared by random primer plus oligo(dT) increased the overall band intensity." @default.
• W1976013973 created "2016-06-24" @default.
• W1976013973 creator A5064979434 @default.
• W1976013973 creator A5091213865 @default.
• W1976013973 date "2003-11-01" @default.
• W1976013973 modified "2023-10-16" @default.
• W1976013973 title "Specific differentiation of recombinant PVYN:O and PVYNTN isolates by multiplex RT-PCR" @default.
• W1976013973 cites W1601516424 @default.
• W1976013973 cites W1963997295 @default.
• W1976013973 cites W1988710560 @default.
• W1976013973 cites W1991108330 @default.
• W1976013973 cites W1993100863 @default.
• W1976013973 cites W1996035067 @default.
• W1976013973 cites W1996234594 @default.
• W1976013973 cites W1997982236 @default.
• W1976013973 cites W2006269250 @default.
• W1976013973 cites W2017691200 @default.
• W1976013973 cites W2030756073 @default.
• W1976013973 cites W2036239910 @default.
• W1976013973 cites W2036666305 @default.
• W1976013973 cites W2048562589 @default.
• W1976013973 cites W2048617418 @default.
• W1976013973 cites W2061974328 @default.
• W1976013973 cites W2066738056 @default.
• W1976013973 cites W2081032932 @default.
• W1976013973 cites W2082186265 @default.
• W1976013973 cites W2082703826 @default.
• W1976013973 cites W2085397883 @default.
• W1976013973 cites W2089073782 @default.
• W1976013973 cites W2091417336 @default.
• W1976013973 cites W2092208463 @default.
• W1976013973 cites W2102372735 @default.
• W1976013973 cites W2123534415 @default.
• W1976013973 cites W2135934689 @default.
• W1976013973 cites W2138257129 @default.
• W1976013973 cites W2159277683 @default.
• W1976013973 cites W2160255421 @default.
• W1976013973 cites W2172268493 @default.
• W1976013973 cites W2334860817 @default.
• W1976013973 cites W2521508417 @default.
• W1976013973 doi "https://doi.org/10.1016/s0166-0934(03)00221-0" @default.
• W1976013973 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14553892" @default.
• W1976013973 hasPublicationYear "2003" @default.
• W1976013973 type Work @default.
• W1976013973 sameAs 1976013973 @default.
• W1976013973 citedByCount "87" @default.
• W1976013973 countsByYear W19760139732012 @default.
• W1976013973 countsByYear W19760139732013 @default.
• W1976013973 countsByYear W19760139732014 @default.
• W1976013973 countsByYear W19760139732015 @default.
• W1976013973 countsByYear W19760139732016 @default.
• W1976013973 countsByYear W19760139732017 @default.
• W1976013973 countsByYear W19760139732019 @default.
• W1976013973 countsByYear W19760139732020 @default.
• W1976013973 countsByYear W19760139732021 @default.
• W1976013973 crossrefType "journal-article" @default.
• W1976013973 hasAuthorship W1976013973A5064979434 @default.
• W1976013973 hasAuthorship W1976013973A5091213865 @default.
• W1976013973 hasConcept C104317684 @default.
• W1976013973 hasConcept C109110057 @default.
• W1976013973 hasConcept C153911025 @default.
• W1976013973 hasConcept C159047783 @default.
• W1976013973 hasConcept C178790620 @default.
• W1976013973 hasConcept C185592680 @default.
• W1976013973 hasConcept C2522874641 @default.
• W1976013973 hasConcept C2776625791 @default.
• W1976013973 hasConcept C2777563447 @default.
• W1976013973 hasConcept C2780504191 @default.
• W1976013973 hasConcept C2781188995 @default.
• W1976013973 hasConcept C40767141 @default.
• W1976013973 hasConcept C49105822 @default.
• W1976013973 hasConcept C54355233 @default.
• W1976013973 hasConcept C86803240 @default.
• W1976013973 hasConcept C90583042 @default.
• W1976013973 hasConceptScore W1976013973C104317684 @default.
• W1976013973 hasConceptScore W1976013973C109110057 @default.
• W1976013973 hasConceptScore W1976013973C153911025 @default.
• W1976013973 hasConceptScore W1976013973C159047783 @default.
• W1976013973 hasConceptScore W1976013973C178790620 @default.
• W1976013973 hasConceptScore W1976013973C185592680 @default.
• W1976013973 hasConceptScore W1976013973C2522874641 @default.
• W1976013973 hasConceptScore W1976013973C2776625791 @default.
• W1976013973 hasConceptScore W1976013973C2777563447 @default.
• W1976013973 hasConceptScore W1976013973C2780504191 @default.
• W1976013973 hasConceptScore W1976013973C2781188995 @default.
• W1976013973 hasConceptScore W1976013973C40767141 @default.
• W1976013973 hasConceptScore W1976013973C49105822 @default.
• W1976013973 hasConceptScore W1976013973C54355233 @default.
• W1976013973 hasConceptScore W1976013973C86803240 @default.
• W1976013973 hasConceptScore W1976013973C90583042 @default.
• W1976013973 hasIssue "2" @default.
• W1976013973 hasLocation W19760139731 @default.
• W1976013973 hasLocation W19760139732 @default.
• W1976013973 hasOpenAccess W1976013973 @default.
• W1976013973 hasPrimaryLocation W19760139731 @default.
• W1976013973 hasRelatedWork W1204177094 @default.
• W1976013973 hasRelatedWork W1980088847 @default.
• W1976013973 hasRelatedWork W2003966165 @default.

• next