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• W1976032825 abstract "A new visible light excitable rhodamine-based Hg 2+ probe has been designed and synthesized. The probe exhibits reversible colorimetric and fluorescent responses towards Hg 2+ . O–N–N–O recognition site in the probe plays a dominant role in forming a complex with 1:1 stoichiometry of Hg 2+ to the probe. Furthermore, the probe has been successfully employed as a fluorescent probe for visualization of Hg 2+ in living HeLa cells. • Highly selective fluorescent probe 1 for Hg 2+ is developed using a rhodamine derivative. • O–N–N–O recognition site in 1 plays a dominant role in forming a 1:1 stoichiometry. • The probe 1 is successfully employed for visualization of Hg 2+ in living HeLa cells. A new visible light excitable fluorescent probe ( 1 ) is synthesized by appending a hydroxymethyl-pyridine to rhodamine B hydrazide. The probe displays very specific Hg 2+ -induced colour change and fluorescent enhancement in the aqueous systems. The “turn-on” response of fluorescence is based on a binding-induced ring-opening process from the spirolactam (nonfluorescent) to acyclic xanthene (fluorescent) in rhodamine B. The coordinating atoms O–N–N–O from the hydroxymethyl-pyridine and rhodamine B hydrazide play dominant role in the formation of a complex with 1:1 stoichiometry of Hg 2+ to 1 . It exhibits a linear response in the range of 0.1–5 μM with the limit of detection (LOD) of 15.7 nM (3 σ /slope), while the calculated value of the association constant of Hg 2+ / 1 is 0.70 × 10 5 M −1 . Furthermore, confocal microscopy imaging experiment demonstrates the probe 1 can be applied as a fluorescent probe for visualization of Hg 2+ in living HeLa cells." @default.
• W1976032825 created "2016-06-24" @default.
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• W1976032825 date "2014-11-01" @default.
• W1976032825 modified "2023-09-30" @default.
• W1976032825 title "A rhodamine-based fluorescent probe for Hg2+ and its application for biological visualization" @default.
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• W1976032825 doi "https://doi.org/10.1016/j.snb.2014.06.104" @default.
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