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- W1976071444 abstract "A simple method is described for affinity purification of radiolabeled antibodies using glutaraldehyde-fixed tumor target cells. The cell-bound antibody fraction is removed from the cells by an acid wash and then immediately subjected to buffer-exchange chromatography. The method was applied to the D3 murine monoclonal antibody which binds to a 290 kDa antigen on the surface of Line 10 guinea pig carcinoma cells. No alteration in the molecular size profile was detected after acid washing. Purification resulted in a significant increase in immunoreactivity by an average of 14 +/- 47% (SD; range 4-30%)." @default.
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- W1976071444 date "1993-04-01" @default.
- W1976071444 modified "2023-09-23" @default.
- W1976071444 title "A simple method for affinity purification of radiolabeled monoclonal antibodies" @default.
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- W1976071444 doi "https://doi.org/10.1016/0969-8051(93)90053-w" @default.
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