FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1976109015> ?p ?o ?g. }

• W1976109015 endingPage "87" @default.
• W1976109015 startingPage "78" @default.
• W1976109015 abstract "Adoptively transferred activated natural killer (A-NK) cells are capable of selectively infiltrating solid tumors, but only at low efficiency when administered systemically. It is unclear if human A-NK cells can be retained in tumor tissue and, if so, what is their action. We investigated intratumor A-NK cell retention and in situ cytokine production, using an xenogeneic ex vivo tissue-isolated tumor model, which permits direct intraarterial infusion.Human colon adenocarcinoma (HT-29) was implanted in the ovarian fat pad of nude rats. The tumors were perfused ex vivo 25 to 30 days postimplant with a known number of cells, and the effluent was collected over time. The number of human A-NK cells and cell surface antigen expression of cells infused and exiting the tumor were calculated, using cell counts and flow cytometry, respectively. Frozen sections were stained with Giemsa and also immunostained for the presence of interleukin-2, -4, and -10, tumor necrosis factor alpha (TNF-alpha), and interferon.Six perfusions with 8 x 10(6) A-NK cells were performed. The mean number of infused A-NK cells that remained in the tumor at the completion of perfusion was 4.74 x 10(6) (59.2%). No differences were noted in cellular phenotype between the infused cells and the cells exiting the tumor: expression of the markers CD45 (97.5% vs 94. 5%), CD14 (0 vs 0), CD3 (3.83% vs 2.83%), and CD56 (86% vs 83%) was unchanged, P > 0.05. Microscopic examination of tumor sections showed tumor surrounded by A-NK cells, with some tumor nests infiltrated by A-NK cells. In situ immunopositivity for interleukin-2 (2/6), interleukin-4 (3/6), interleukin-10 (2/6), and TNF-alpha (2/6) specimens was observed. Immunostaining for interferon-gamma was negative. Conclusions. The retention of A-NK cells in the transplanted human colon tumor tissue was found to be efficient (59.2 %) in this model. Although perfusion time was limited, A-NK cells were able to infiltrate the tumor and initiate cytokine production." @default.
• W1976109015 created "2016-06-24" @default.
• W1976109015 creator A5013783152 @default.
• W1976109015 creator A5050172772 @default.
• W1976109015 creator A5067541948 @default.
• W1976109015 creator A5070231669 @default.
• W1976109015 creator A5075501671 @default.
• W1976109015 creator A5079608944 @default.
• W1976109015 date "1999-03-01" @default.
• W1976109015 modified "2023-10-01" @default.
• W1976109015 title "Activated Natural Killer Cell Tumor Retention and Cytokine Production in Colon Tumor Using a Tissue-Isolated Model" @default.
• W1976109015 cites W1488558875 @default.
• W1976109015 cites W1490694960 @default.
• W1976109015 cites W1504887220 @default.
• W1976109015 cites W1534220439 @default.
• W1976109015 cites W1539383686 @default.
• W1976109015 cites W1556867369 @default.
• W1976109015 cites W1568169604 @default.
• W1976109015 cites W1588270487 @default.
• W1976109015 cites W159503907 @default.
• W1976109015 cites W1651198898 @default.
• W1976109015 cites W1970001272 @default.
• W1976109015 cites W1971037354 @default.
• W1976109015 cites W1983299898 @default.
• W1976109015 cites W1984404339 @default.
• W1976109015 cites W1991148746 @default.
• W1976109015 cites W2000943484 @default.
• W1976109015 cites W2000989438 @default.
• W1976109015 cites W2023027189 @default.
• W1976109015 cites W2041701302 @default.
• W1976109015 cites W2053029785 @default.
• W1976109015 cites W2062799513 @default.
• W1976109015 cites W2069078625 @default.
• W1976109015 cites W2070684861 @default.
• W1976109015 cites W2082409286 @default.
• W1976109015 cites W2084093106 @default.
• W1976109015 cites W2087553718 @default.
• W1976109015 cites W2097641815 @default.
• W1976109015 cites W2104417744 @default.
• W1976109015 cites W2115112315 @default.
• W1976109015 cites W2127022844 @default.
• W1976109015 cites W2178740741 @default.
• W1976109015 cites W2314331148 @default.
• W1976109015 doi "https://doi.org/10.1006/jsre.1998.5521" @default.
• W1976109015 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10068530" @default.
• W1976109015 hasPublicationYear "1999" @default.
• W1976109015 type Work @default.
• W1976109015 sameAs 1976109015 @default.
• W1976109015 citedByCount "5" @default.
• W1976109015 crossrefType "journal-article" @default.
• W1976109015 hasAuthorship W1976109015A5013783152 @default.
• W1976109015 hasAuthorship W1976109015A5050172772 @default.
• W1976109015 hasAuthorship W1976109015A5067541948 @default.
• W1976109015 hasAuthorship W1976109015A5070231669 @default.
• W1976109015 hasAuthorship W1976109015A5075501671 @default.
• W1976109015 hasAuthorship W1976109015A5079608944 @default.
• W1976109015 hasConcept C142724271 @default.
• W1976109015 hasConcept C150903083 @default.
• W1976109015 hasConcept C154317977 @default.
• W1976109015 hasConcept C17991360 @default.
• W1976109015 hasConcept C202751555 @default.
• W1976109015 hasConcept C203014093 @default.
• W1976109015 hasConcept C207001950 @default.
• W1976109015 hasConcept C26291073 @default.
• W1976109015 hasConcept C2778102761 @default.
• W1976109015 hasConcept C2778690821 @default.
• W1976109015 hasConcept C3020616263 @default.
• W1976109015 hasConcept C502942594 @default.
• W1976109015 hasConcept C55493867 @default.
• W1976109015 hasConcept C70905583 @default.
• W1976109015 hasConcept C71924100 @default.
• W1976109015 hasConcept C86803240 @default.
• W1976109015 hasConceptScore W1976109015C142724271 @default.
• W1976109015 hasConceptScore W1976109015C150903083 @default.
• W1976109015 hasConceptScore W1976109015C154317977 @default.
• W1976109015 hasConceptScore W1976109015C17991360 @default.
• W1976109015 hasConceptScore W1976109015C202751555 @default.
• W1976109015 hasConceptScore W1976109015C203014093 @default.
• W1976109015 hasConceptScore W1976109015C207001950 @default.
• W1976109015 hasConceptScore W1976109015C26291073 @default.
• W1976109015 hasConceptScore W1976109015C2778102761 @default.
• W1976109015 hasConceptScore W1976109015C2778690821 @default.
• W1976109015 hasConceptScore W1976109015C3020616263 @default.
• W1976109015 hasConceptScore W1976109015C502942594 @default.
• W1976109015 hasConceptScore W1976109015C55493867 @default.
• W1976109015 hasConceptScore W1976109015C70905583 @default.
• W1976109015 hasConceptScore W1976109015C71924100 @default.
• W1976109015 hasConceptScore W1976109015C86803240 @default.
• W1976109015 hasIssue "1" @default.
• W1976109015 hasLocation W19761090151 @default.
• W1976109015 hasLocation W19761090152 @default.
• W1976109015 hasOpenAccess W1976109015 @default.
• W1976109015 hasPrimaryLocation W19761090151 @default.
• W1976109015 hasRelatedWork W1820832512 @default.
• W1976109015 hasRelatedWork W2006380937 @default.
• W1976109015 hasRelatedWork W2011077733 @default.
• W1976109015 hasRelatedWork W2046516920 @default.
• W1976109015 hasRelatedWork W2066485035 @default.

• next