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- W1976128575 abstract "A two-phase biotransformation process for selective hydroxylation of n-octane to 1-octanol via Pseudomonas putida KT2440 harboring heterologously expressed P450 monooxygenase from Mycobacterium marinum is presented. Maximum cell-specific conversion rates of 12.7 mgoctanol gCDWh–1 were observed not only in shaking flasks but also in 3.7-L-bioreactor studies. The bioreactor experiments were performed avoiding explosive gas mixtures by lowering volumetric power input, aeration rates and substrate concentrations. Based on a stoichiometric network of P. putida KT2440 topological studies were carried out. As a conclusion, potential limitations of NAD(P)H and/or ATP supply at production conditions can be excluded. Hence, the great potential of the host for further increasing conversion is outlined." @default.
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- W1976128575 date "2013-04-25" @default.
- W1976128575 modified "2023-10-18" @default.
- W1976128575 title "Production of 1-Octanol from<i>n</i>-Octane by<i>Pseudomonas putida</i>KT2440" @default.
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- W1976128575 doi "https://doi.org/10.1002/cite.201200178" @default.
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