Matches in SemOpenAlex for { <https://semopenalex.org/work/W1976172015> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W1976172015 endingPage "324" @default.
- W1976172015 startingPage "315" @default.
- W1976172015 abstract "Two lectins were isolated from Canavalia ensiformis and Dioclea grandiflora seeds. Gel filtration produced a fraction corresponding to Con A or D. grandiflora lectin while erythroagglutination assays revealed a distinct fraction presenting a lectin that agglutinates human red blood cells (RBCs) but not rabbit RBCs. Hydrophobic interaction chromatography showed that the latter fraction yielded a protein that readily agglutinates human erythrocytes; the lectin was also purified by affinity chromatography on Lac-Sepharose showing similar properties to that of the Phenyl-Sepharose-purified lectin. Despite minor differences (carbohydrate content or A1%1cm), the two lectins showed similar molecular properties in that they consisted of two non-covalently linked monomers having a Mr of 29-30 kDa and their pI values indicated that both lectins were slightly acidic proteins. The C. ensiformis lectin (CEL-II) and D. grandiflora lectin (DGL-II) specifically recognised the H-type 2 blood group (alpha-L-Fuc (1-2)-beta-D-Gal (1-4)-beta-D-GlcNAc-O-R), while binding to H-type 1, H-type 3, H-type 4, Leª or Le y was weaker. Carbohydrate inhibition of erythroagglutination showed that simple sugars were weakly recognised by the lectins, if at all. The N-terminal region presented a unique sequence hitherto found only in some Diocleinae lectins (designated type II). The overall results confirmed the existence of a second distinct lectin type, phylogenetically close to Diocleinae species. The data indicate a functional similarity among lectins of this type which possesses distinctive characteristics differentiating them from classical Man/Glc lectins." @default.
- W1976172015 created "2016-06-24" @default.
- W1976172015 creator A5010758698 @default.
- W1976172015 creator A5028936591 @default.
- W1976172015 creator A5084596169 @default.
- W1976172015 date "2005-09-01" @default.
- W1976172015 modified "2023-10-12" @default.
- W1976172015 title "Isolation and characterization of novel lectins from Canavalia ensiformis DC and Dioclea grandiflora Mart. ex Benth. seeds" @default.
- W1976172015 cites W1483264458 @default.
- W1976172015 cites W1548133588 @default.
- W1976172015 cites W1932052686 @default.
- W1976172015 cites W1968731126 @default.
- W1976172015 cites W1974127992 @default.
- W1976172015 cites W1975085160 @default.
- W1976172015 cites W1977264327 @default.
- W1976172015 cites W1992697991 @default.
- W1976172015 cites W2012221275 @default.
- W1976172015 cites W2014181077 @default.
- W1976172015 cites W2017075198 @default.
- W1976172015 cites W2018958401 @default.
- W1976172015 cites W2026533254 @default.
- W1976172015 cites W2033708696 @default.
- W1976172015 cites W2041735405 @default.
- W1976172015 cites W2047625580 @default.
- W1976172015 cites W2048214434 @default.
- W1976172015 cites W2048966080 @default.
- W1976172015 cites W2063053206 @default.
- W1976172015 cites W2068809301 @default.
- W1976172015 cites W2080416235 @default.
- W1976172015 cites W2098176907 @default.
- W1976172015 cites W2100837269 @default.
- W1976172015 cites W2141121550 @default.
- W1976172015 cites W2164358316 @default.
- W1976172015 doi "https://doi.org/10.1590/s1677-04202005000300006" @default.
- W1976172015 hasPublicationYear "2005" @default.
- W1976172015 type Work @default.
- W1976172015 sameAs 1976172015 @default.
- W1976172015 citedByCount "11" @default.
- W1976172015 countsByYear W19761720152013 @default.
- W1976172015 countsByYear W19761720152017 @default.
- W1976172015 countsByYear W19761720152018 @default.
- W1976172015 countsByYear W19761720152020 @default.
- W1976172015 countsByYear W19761720152021 @default.
- W1976172015 crossrefType "journal-article" @default.
- W1976172015 hasAuthorship W1976172015A5010758698 @default.
- W1976172015 hasAuthorship W1976172015A5028936591 @default.
- W1976172015 hasAuthorship W1976172015A5084596169 @default.
- W1976172015 hasBestOaLocation W19761720151 @default.
- W1976172015 hasConcept C179247698 @default.
- W1976172015 hasConcept C181199279 @default.
- W1976172015 hasConcept C185592680 @default.
- W1976172015 hasConcept C193363816 @default.
- W1976172015 hasConcept C2776490341 @default.
- W1976172015 hasConcept C2778977261 @default.
- W1976172015 hasConcept C2779881004 @default.
- W1976172015 hasConcept C2780758401 @default.
- W1976172015 hasConcept C55493867 @default.
- W1976172015 hasConcept C86803240 @default.
- W1976172015 hasConceptScore W1976172015C179247698 @default.
- W1976172015 hasConceptScore W1976172015C181199279 @default.
- W1976172015 hasConceptScore W1976172015C185592680 @default.
- W1976172015 hasConceptScore W1976172015C193363816 @default.
- W1976172015 hasConceptScore W1976172015C2776490341 @default.
- W1976172015 hasConceptScore W1976172015C2778977261 @default.
- W1976172015 hasConceptScore W1976172015C2779881004 @default.
- W1976172015 hasConceptScore W1976172015C2780758401 @default.
- W1976172015 hasConceptScore W1976172015C55493867 @default.
- W1976172015 hasConceptScore W1976172015C86803240 @default.
- W1976172015 hasIssue "3" @default.
- W1976172015 hasLocation W19761720151 @default.
- W1976172015 hasOpenAccess W1976172015 @default.
- W1976172015 hasPrimaryLocation W19761720151 @default.
- W1976172015 hasRelatedWork W1978025593 @default.
- W1976172015 hasRelatedWork W1978669987 @default.
- W1976172015 hasRelatedWork W2010902183 @default.
- W1976172015 hasRelatedWork W2016617621 @default.
- W1976172015 hasRelatedWork W204093968 @default.
- W1976172015 hasRelatedWork W2052291987 @default.
- W1976172015 hasRelatedWork W2095128775 @default.
- W1976172015 hasRelatedWork W2097492335 @default.
- W1976172015 hasRelatedWork W2294584344 @default.
- W1976172015 hasRelatedWork W2914365049 @default.
- W1976172015 hasVolume "17" @default.
- W1976172015 isParatext "false" @default.
- W1976172015 isRetracted "false" @default.
- W1976172015 magId "1976172015" @default.
- W1976172015 workType "article" @default.