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- W1976230851 abstract "Expanding the eukaryotic genetic code to include unnatural amino acids with novel properties would provide powerful tools for manipulating protein function in eukaryotic cells. Toward this goal, a general approach with potential for isolating aminoacyl-tRNA synthetases that incorporate unnatural amino acids with high fidelity into proteins in Saccharomyces cerevisiae is described. The method is based on activation of GAL4-responsive HIS3, URA3, or lacZ reporter genes by suppression of amber codons in GAL4. The optimization of GAL4 reporters is described, and the positive and negative selection of active Escherichia coli tyrosyl-tRNA synthetase (EcTyrRS)/tRNACUA is demonstrated. Importantly, both selections can be performed on a single cell and with a range of stringencies. This method will facilitate the isolation of a range of aminoacyl-tRNA synthetase (aaRS)/tRNACUA activities from large libraries of mutant synthetases." @default.
- W1976230851 created "2016-06-24" @default.
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- W1976230851 date "2003-06-01" @default.
- W1976230851 modified "2023-10-16" @default.
- W1976230851 title "Progress Toward an Expanded Eukaryotic Genetic Code" @default.
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- W1976230851 doi "https://doi.org/10.1016/s1074-5521(03)00123-6" @default.
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