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• W1976267257 abstract "Purpose of study: Although several bone morphogenic proteins (BMPs) have been shown to enhance bone healing and spinal fusion, the optimal BMPs or combination of BMPs to promote fusion are unknown. This study was designed to comprehensively elucidate the distinct and potentially synergistic osteogenic activity of 14 types of BMPs at various stages of osteogenesis. Methods used: Recombinant adenoviruses expressing 14 human BMPs (ie, BMP-2 to BMP-15) were constructed and used to infect both pluripotent mesenchymal progenitor C3H10T1/2 cells and committed osteoblastic C2C12 cells. The osteogenic activity was determined by measuring the induction of alkaline phosphatase, osteocalcin and matrix mineralization on BMP stimulation. of findings: BMP-2, -4, -6, -7 and -9 significantly induced alkaline phosphatase activity in C2C12 osteoblastic cells. BMP-5, -8, -10, -11, -12 and -13 exerted weak induction of alkaline phosphatase. However, only BMP-2, -6 and -9 significantly induced alkaline phosphatase activity in C3H10T1/2 pluripotent cells, although weak induction by BMP4 and 7 was detected. Histochemical staining assays demonstrated that BMP-6 and -9 induced the greatest increase in alkaline phosphatase staining, whereas BMP-2 and -4 (and BMP-7 to lesser extent) induced a modest increase in alkaline phosphatase activity in C2C12 cells. BMP-2, -6 and -9 significantly induced osteocalcin expression (ie, a late marker of osteogenesis) in C3H10T1/2 cells, and BMP-4 and -7 slightly increased osteocalcin expression. In C2C12 cells osteocalcin expression was strongly induced by BMP-2, -4, -6, -7 and -9, whereas BMP-5, -10 and -14 slightly induced expression. Mineralized nodules were readily detected in the C3H10T1/2 cells infected with BMP-2, -6 and -9 vectors (and, to lesser extent, with BMP-4 -7 and -10). Finally, we observed strong synergistic effects among the BMPs that had an ability to activate the osteogenic markers. Interestingly, a strong synergistic effect on osteogenesis was detected in cells stimulated by BMP-5 plus -10, BMP-5 plus -12, BMP-5 plus -13, BMP-7 plus -10 or BMP-7 plus -13. Relationship between findings and existing knowledge: We have conducted a comprehensive analysis of the osteogenic activity of 14 types of BMPs in osteoblastic progenitor cells and demonstrated that BMP-2, -6 and -9 are the most potent osteogenic factors, whereas BMP-4 and -7 exhibit reasonably strong osteoinductive activity. Further, certain combinations of BMPs exert a strong synergy on osteogenesis. Overall significance of findings: These findings have important implications for the development of effective new formulas for bone healing and spine fusion. Disclosures: No disclosures. Conflict of interest: Tong-Chuan He, grant research support." @default.
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• W1976267257 date "2002-09-01" @default.
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• W1976267257 title "2:20 The osteogenic activity of 14 types of bone morphogenic proteins" @default.
• W1976267257 doi "https://doi.org/10.1016/s1529-9430(02)00347-9" @default.
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