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- W1976323873 abstract "Separation of previously postulated chlorophyll components in vivo was attempted by fractional extraction of chloroplasts with acetone and methanol of various concentration. Dilute solvents were found to extract more or, less intact chlorophyllcarrier complexes; more concentrated solvents dissociate these complexes, first in the extracts, then also in the residual chloroplast material. The absorption and the fluorescence spectra of the extracts and the residues further suggest a partial separation of two types of complexes, with the “long-wave” component, chlorophyll a 683, extracted ahead of the “short-wave” component, chlorophyll a 668. Particularly revealing are the fluorescence spectra at −196°; it seems that at this temperature all chlorophyll a complexes become fluorescent (while only one fluoresces strongly at room temperature). The relative intensities of the fluorescence maxima at 685 nm, 696–700 nm, and 735 nm change with solvent concentration, suggesting partial separation of the complexes." @default.
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- W1976323873 date "1966-06-01" @default.
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- W1976323873 title "Absorption and fluorescence spectra of spinach chloroplast fractions obtained by solvent extraction" @default.
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- W1976323873 doi "https://doi.org/10.1016/0926-6585(66)90344-x" @default.
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