FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1976409035> ?p ?o ?g. }

• W1976409035 endingPage "546" @default.
• W1976409035 startingPage "531" @default.
• W1976409035 abstract "Recent studies have demonstrated the importance of heptad repeat regions within envelope proteins of viruses in mediating conformational changes at various stages of viral infection. However, it is not clear if heptad repeats have a direct role in the actual fusion event. Here we have synthesized, fluorescently labeled and functionally and structurally characterized a wild-type 70 residue peptide (SV-117) composed of both the fusion peptide and the N-terminal heptad repeat of Sendai virus fusion protein, two of its mutants, as well as the fusion peptide and heptad repeat separately. One mutation was introduced in the fusion peptide (G119K) and another in the heptad repeat region (I154K). Similar mutations have been shown to drastically reduce the fusogenic ability of the homologous fusion protein of Newcastle disease virus. We found that only SV-117 was active in inducing lipid mixing of egg phosphatidylcholine/phosphatidyiglycerol (PC/PG) large unilamellar vesicles (LUV), and not the mutants nor the mixture of the fusion peptide and the heptad repeat. Functional characterization revealed that SV-117, and to a lesser extent its two mutants, were potent inhibitors of Sendai virus-mediated hemolysis of red blood cells, while the fusion peptide and SV-150 were negligibly active alone or in a mixture. Hemagglutinin assays revealed that none of the peptides disturb the binding of virions to red blood cells. Further studies revealed that SV-117 and its mutants oligomerize similarly in solution and in membrane, and have similar potency in inducing vesicle aggregation. Circular dichroism and FTIR spectroscopy revealed a higher helical content for SV-117 compared to its mutants in 40 % tifluorethanol and in PC/PG multibilayer membranes, respectively, ATR-FTIR studies indicated that SV-117 lies more parallel with the surface of the membrane than its mutants. These observations suggest a direct role for the N-terminal heptad repeat in assisting the fusion peptide in mediating membrane fusion." @default.
• W1976409035 created "2016-06-24" @default.
• W1976409035 creator A5042391100 @default.
• W1976409035 creator A5077610815 @default.
• W1976409035 date "1999-09-01" @default.
• W1976409035 modified "2023-10-04" @default.
• W1976409035 title "Direct Evidence that the N-Terminal Heptad Repeat of Sendai Virus Fusion Protein Participates in Membrane Fusion" @default.
• W1976409035 cites W1487158785 @default.
• W1976409035 cites W1503300581 @default.
• W1976409035 cites W1506255624 @default.
• W1976409035 cites W1513897103 @default.
• W1976409035 cites W1518606102 @default.
• W1976409035 cites W1526559406 @default.
• W1976409035 cites W1564140172 @default.
• W1976409035 cites W1568177357 @default.
• W1976409035 cites W1568417978 @default.
• W1976409035 cites W1600024407 @default.
• W1976409035 cites W1623183061 @default.
• W1976409035 cites W1895313693 @default.
• W1976409035 cites W1903694863 @default.
• W1976409035 cites W1905114611 @default.
• W1976409035 cites W1922968569 @default.
• W1976409035 cites W1966982471 @default.
• W1976409035 cites W1969287991 @default.
• W1976409035 cites W1974053173 @default.
• W1976409035 cites W1974446403 @default.
• W1976409035 cites W1976122531 @default.
• W1976409035 cites W1977356160 @default.
• W1976409035 cites W1978045911 @default.
• W1976409035 cites W1983145685 @default.
• W1976409035 cites W1990016062 @default.
• W1976409035 cites W1990502906 @default.
• W1976409035 cites W1992683016 @default.
• W1976409035 cites W1993464608 @default.
• W1976409035 cites W2000393599 @default.
• W1976409035 cites W2005780220 @default.
• W1976409035 cites W2006591389 @default.
• W1976409035 cites W2007332317 @default.
• W1976409035 cites W2008562844 @default.
• W1976409035 cites W2010814343 @default.
• W1976409035 cites W2011483956 @default.
• W1976409035 cites W2012735516 @default.
• W1976409035 cites W2016408696 @default.
• W1976409035 cites W2021017618 @default.
• W1976409035 cites W2022340574 @default.
• W1976409035 cites W2023054692 @default.
• W1976409035 cites W2023807605 @default.
• W1976409035 cites W2025430406 @default.
• W1976409035 cites W2027620968 @default.
• W1976409035 cites W2029122944 @default.
• W1976409035 cites W2031179671 @default.
• W1976409035 cites W2031780593 @default.
• W1976409035 cites W2031784324 @default.
• W1976409035 cites W2033757712 @default.
• W1976409035 cites W2034730732 @default.
• W1976409035 cites W2036656105 @default.
• W1976409035 cites W2038110814 @default.
• W1976409035 cites W2038679282 @default.
• W1976409035 cites W2040105288 @default.
• W1976409035 cites W2040211368 @default.
• W1976409035 cites W2043140513 @default.
• W1976409035 cites W2048235504 @default.
• W1976409035 cites W2053324553 @default.
• W1976409035 cites W2056976674 @default.
• W1976409035 cites W2063701397 @default.
• W1976409035 cites W2071172219 @default.
• W1976409035 cites W2071507299 @default.
• W1976409035 cites W2071684237 @default.
• W1976409035 cites W2076156268 @default.
• W1976409035 cites W2076462632 @default.
• W1976409035 cites W2077963467 @default.
• W1976409035 cites W2077999682 @default.
• W1976409035 cites W2089173889 @default.
• W1976409035 cites W2090107903 @default.
• W1976409035 cites W2091585240 @default.
• W1976409035 cites W2092571716 @default.
• W1976409035 cites W2100049032 @default.
• W1976409035 cites W2107137018 @default.
• W1976409035 cites W2114713553 @default.
• W1976409035 cites W2128002736 @default.
• W1976409035 cites W2139555392 @default.
• W1976409035 cites W2144892927 @default.
• W1976409035 cites W2155916246 @default.
• W1976409035 cites W2158622538 @default.
• W1976409035 cites W2159031835 @default.
• W1976409035 cites W2164631754 @default.
• W1976409035 cites W2164868461 @default.
• W1976409035 cites W2168799539 @default.
• W1976409035 cites W2170984093 @default.
• W1976409035 cites W2175092423 @default.
• W1976409035 cites W2175996949 @default.
• W1976409035 cites W2182660457 @default.
• W1976409035 cites W38281283 @default.
• W1976409035 cites W4211235955 @default.
• W1976409035 cites W4237410627 @default.
• W1976409035 cites W83274283 @default.
• W1976409035 cites W879007266 @default.
• W1976409035 doi "https://doi.org/10.1006/jmbi.1999.3097" @default.

• next