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- W1976425397 abstract "Through specialised structures called haustoria, filamentous eukaryotic plant pathogenssuch as rusts and mildews can deliver “effector” proteins directly into plant cells in order tomanipulate specific defence responses and processes. Hyaloperonospora arabidopsidis (Hpa)is a haustorium-producing biotrophic oomycete pathogen that causes downy mildew onArabidopsis. Over 100 candidate effectors (called “HaRxLs”), carrying a characteristic RxLRprotein motif, have been predicted from the Hpa genome sequence. This PhD work focusedon the characterisation of some of these effectors and their interaction with plant defences.First, the Pseudomonas syringae pv. tomato (Pst) strain DC3000 type III secretion systemwas used to examine the effect of single oomycete effectors in planta. One of these, ATR13from Hpa isolate Emco5 (ATR13Emco5) has been shown to be recognised in some Arabidopsisaccessions by the RPP13 protein, preventing Hpa Emco5 from completing its life cycle onsuch resistant plants. Hpa Emco5 can however complete its life cycle on Ws-0 butparadoxically, ATR13Emco5 is also recognised in this accession. Here, I show that ATR13Emco5is weakly recognised in Ws-0 by a single RPP13-independent, EDS1-independent dominantgene called RHA13, shown by rough mapping to position on Arabidopsis chromosome 4.Second, I contributed to two independent functional screens performed in our laboratory toexperimentally characterise the Hpa effectorome. The first screen focused on HaRxLs effectson bacteria virulence using the EDV system. The second screen was based on HaRxLssubcellular localisation using Agrobacterium-mediated transient expression. As presented inthis work, I showed that both screens identified putative interesting effectors which increasedplant susceptibility to Pst and Hpa and localised to various plant subcellular compartments.One particular effector candidate, HaRxL79, localised to microtubules in planta andinteracted with two microtubule-associated proteins in a yeast-two-hybrid assay. HaRxL79was also found to interact with Arabidopsis histone chaperones (AtNAP1s) in the plantcytoplasm, which were observed to play a role in plant susceptibility to Hpa and partially tothe necrotroph Botrytis cinerea, but not to the hemibiotrophs Pst and Phytophthoraparasitica. AtNAP1s also interact with HaRxL67, a vacuole-associated Hpa effector. Fromthis, I propose that AtNAP1s are susceptibility factors for Hpa, specifically targeted by Hpaeffectors HaRxL67 and HaRxL79 in order to promote susceptibility and maintain biotrophy." @default.
- W1976425397 created "2016-06-24" @default.
- W1976425397 creator A5083341025 @default.
- W1976425397 date "2011-12-01" @default.
- W1976425397 modified "2023-09-23" @default.
- W1976425397 title "Investigating the role of downy mildew effectors in host resistance and susceptibility" @default.
- W1976425397 hasPublicationYear "2011" @default.
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