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- W1976425447 abstract "The attenuated total reflection fourier transform infrared spectroscopy (ATR-FTIR) allows a detailed analysis of surface attached molecules, including their secondary structure, reaction mechanism, orientation and interaction with small molecules or proteins [1]. The aim of our study is the development of a universal immobilization technique on germanium for proteins with different tags or residues (e. g.: his-tag, strep-tag, cysteine, amine, etc.). The first step was the organic synthesis of triethoxysilanes with modified functional head groups (e. g.: maleimido, keton, succinimidyl-ester). The attachment of the triethoxysilanes on germanium was monitored by ATR-FTIR and X-ray-photoelectron-spectroscopy [2]. By a reaction of the succinimidyl-ester with amino-nitrilotriacetic acid a carboxylic terminated surface, which coordinates divalent cations like Ni2+, was created. As an example, the small GTPase Ras was attached to the carboxylated surface via his-tag [3]. The cellular function of active Ras as molecular switch was shown by a berylliumfluoride titration assay, which allows the on and off switching of Ras at atomic resolution by means of difference-spectroscopy [3]. The treatment with imidazole removes over 80 % of the attached proteins and allows a repeated binding of Ras [3]. Furthermore, the activity of immobilized Photosystem I (PSI) was proven by light-induced difference spectroscopy [3]. In comparison with recent techniques, the attachment and analysis of transmembrane proteins becomes accessible, because measurements in detergent are now possible. Further advantages are the reusability of the surface, a better signal to noise ratio and the covalent functionalization of germanium, which leads to a more stable attachment.References:[1]: Kotting C. et al., Chemical Physics, 2011, 396, 72-83.[2]: Devouge S. et al., Journal of Colloid and Interface Science, 2009, 332, 408-415.[3]: Schartner J. et al., J. Am. Chem. Soc., 2013, 135, 4079-4087." @default.
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- W1976425447 date "2014-01-01" @default.
- W1976425447 modified "2023-09-30" @default.
- W1976425447 title "Protein Immobilization on Chemically Functionalized Germanium Investigated by ATR-FTIR" @default.
- W1976425447 doi "https://doi.org/10.1016/j.bpj.2013.11.1225" @default.
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