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- W1976525596 abstract "The first X-ray structure of the eukaryotic ribosome at 3.0 Å resolution was determined using ribosomes isolated and crystallized from the yeast Saccharomyces cerevisiae (Ben-Shem A, Garreau de Loubresse N, Melnikov S, Jenner L, Yusupova G, Yusupov M: The structure of the eukaryotic ribosome at 3.0 A resolution. Science 2011, 334:1524–1529). This accomplishment was possible due to progress in yeast ribosome biochemistry as well as recent advances in crystallographic methods developed for structure determination of prokaryotic ribosomes isolated from Thermus thermophilus and Escherichia coli. In this review we will focus on the development of isolation procedures that allowed structure determination (both cryo-EM and X-ray crystallography) to be successful for the yeast S. cerevisiae. Additionally we will introduce a new nomenclature that facilitates comparison of ribosomes from different species and kingdoms of life. Finally we will discuss the impact of the yeast 80S ribosome crystal structure on perspectives for future investigations." @default.
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- W1976525596 date "2012-12-01" @default.
- W1976525596 modified "2023-10-18" @default.
- W1976525596 title "Crystal structure of the 80S yeast ribosome" @default.
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- W1976525596 doi "https://doi.org/10.1016/j.sbi.2012.07.013" @default.
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