FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1976549433> ?p ?o ?g. }

• W1976549433 endingPage "1178" @default.
• W1976549433 startingPage "1171" @default.
• W1976549433 abstract "A density gradient electrophoresis (DGE) apparatus (2.2 x, 14 cm) was constructed for the rapid separation of milligram quantities of proteins. By using binary buffers according to Bier (Electrophoresis 1993, 14, 1011-1018) proteins were rate-zonally separated in less than 60 min. Acidic proteins were separated in a pH 8.6, 56 microS/cm buffer, and basic proteins in a pH 5.4, 76 microS/cm buffer. Thus the A (pI 5.15) and B (pI 5.30) forms of beta-lactoglobulin as well as the sialylated glycoforms of apotransferrin were well separated at pH 8.6. The isoforms of myoglobin (pI 6.9 and 7.35, respectively), RNAse A (pI 9.45) and cytochrome c (pI 10.0) and lysozyme (pI 11) were separated at pH 5.4 within 80 min. On a 7 cm DGE column, subcellular organelles derived from HeLa cells were separated in standard electrophoresis buffer (655 microS/cm) for 90 min at 10 mA. Using a new low conductivity buffer (193 microS/cm) 20 min was sufficient to separate late endosomes, lysosomes, endoplasmic reticulum, early endosomes, plasma membrane, clathrin-coated pits, proteasomes, and clathrin-coated vesicles within a single run directly from a postnuclear supernatant." @default.
• W1976549433 created "2016-06-24" @default.
• W1976549433 creator A5015027483 @default.
• W1976549433 creator A5019907039 @default.
• W1976549433 creator A5020233877 @default.
• W1976549433 creator A5069505817 @default.
• W1976549433 creator A5074401427 @default.
• W1976549433 creator A5075250909 @default.
• W1976549433 date "1998-06-01" @default.
• W1976549433 modified "2023-10-17" @default.
• W1976549433 title "High performance density gradient electrophoresis of subcellular organelles, protein complexes and proteins" @default.
• W1976549433 cites W1503078944 @default.
• W1976549433 cites W1508243224 @default.
• W1976549433 cites W1515765303 @default.
• W1976549433 cites W1520733676 @default.
• W1976549433 cites W1554967593 @default.
• W1976549433 cites W1908408342 @default.
• W1976549433 cites W1964629305 @default.
• W1976549433 cites W1966796589 @default.
• W1976549433 cites W1967698568 @default.
• W1976549433 cites W1978901045 @default.
• W1976549433 cites W1983310201 @default.
• W1976549433 cites W1985104644 @default.
• W1976549433 cites W1988147667 @default.
• W1976549433 cites W2015373718 @default.
• W1976549433 cites W2028088263 @default.
• W1976549433 cites W2031469085 @default.
• W1976549433 cites W2038435335 @default.
• W1976549433 cites W2047870189 @default.
• W1976549433 cites W2048039010 @default.
• W1976549433 cites W2051609162 @default.
• W1976549433 cites W2056024327 @default.
• W1976549433 cites W2056410257 @default.
• W1976549433 cites W2058157433 @default.
• W1976549433 cites W2061462231 @default.
• W1976549433 cites W2068149161 @default.
• W1976549433 cites W2076537666 @default.
• W1976549433 cites W2077130252 @default.
• W1976549433 cites W2083227510 @default.
• W1976549433 cites W2085339797 @default.
• W1976549433 cites W2094646376 @default.
• W1976549433 cites W2100837269 @default.
• W1976549433 cites W2106863157 @default.
• W1976549433 cites W2113717191 @default.
• W1976549433 cites W2135079499 @default.
• W1976549433 cites W2137291946 @default.
• W1976549433 cites W4293247451 @default.
• W1976549433 doi "https://doi.org/10.1002/elps.1150190718" @default.
• W1976549433 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9662180" @default.
• W1976549433 hasPublicationYear "1998" @default.
• W1976549433 type Work @default.
• W1976549433 sameAs 1976549433 @default.
• W1976549433 citedByCount "9" @default.
• W1976549433 crossrefType "journal-article" @default.
• W1976549433 hasAuthorship W1976549433A5015027483 @default.
• W1976549433 hasAuthorship W1976549433A5019907039 @default.
• W1976549433 hasAuthorship W1976549433A5020233877 @default.
• W1976549433 hasAuthorship W1976549433A5069505817 @default.
• W1976549433 hasAuthorship W1976549433A5074401427 @default.
• W1976549433 hasAuthorship W1976549433A5075250909 @default.
• W1976549433 hasConcept C102747710 @default.
• W1976549433 hasConcept C116084860 @default.
• W1976549433 hasConcept C121332964 @default.
• W1976549433 hasConcept C12554922 @default.
• W1976549433 hasConcept C130316041 @default.
• W1976549433 hasConcept C144647389 @default.
• W1976549433 hasConcept C154337534 @default.
• W1976549433 hasConcept C158617107 @default.
• W1976549433 hasConcept C168240541 @default.
• W1976549433 hasConcept C170493617 @default.
• W1976549433 hasConcept C181199279 @default.
• W1976549433 hasConcept C185592680 @default.
• W1976549433 hasConcept C2776966407 @default.
• W1976549433 hasConcept C2778892434 @default.
• W1976549433 hasConcept C40250595 @default.
• W1976549433 hasConcept C41625074 @default.
• W1976549433 hasConcept C43617362 @default.
• W1976549433 hasConcept C50476208 @default.
• W1976549433 hasConcept C53009064 @default.
• W1976549433 hasConcept C55493867 @default.
• W1976549433 hasConcept C62520636 @default.
• W1976549433 hasConcept C63162447 @default.
• W1976549433 hasConcept C86803240 @default.
• W1976549433 hasConceptScore W1976549433C102747710 @default.
• W1976549433 hasConceptScore W1976549433C116084860 @default.
• W1976549433 hasConceptScore W1976549433C121332964 @default.
• W1976549433 hasConceptScore W1976549433C12554922 @default.
• W1976549433 hasConceptScore W1976549433C130316041 @default.
• W1976549433 hasConceptScore W1976549433C144647389 @default.
• W1976549433 hasConceptScore W1976549433C154337534 @default.
• W1976549433 hasConceptScore W1976549433C158617107 @default.
• W1976549433 hasConceptScore W1976549433C168240541 @default.
• W1976549433 hasConceptScore W1976549433C170493617 @default.
• W1976549433 hasConceptScore W1976549433C181199279 @default.
• W1976549433 hasConceptScore W1976549433C185592680 @default.
• W1976549433 hasConceptScore W1976549433C2776966407 @default.
• W1976549433 hasConceptScore W1976549433C2778892434 @default.
• W1976549433 hasConceptScore W1976549433C40250595 @default.

• next