Matches in SemOpenAlex for { <https://semopenalex.org/work/W1976562425> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W1976562425 endingPage "106" @default.
- W1976562425 startingPage "97" @default.
- W1976562425 abstract "DNA was subjected to bisulfite-catalyzed transamination at the N4 sites of its cytosine residues with 1,8-diaminooctane (DAO). The product, DNA-DAO, was non-specifically degraded with a cloned staphylococcal nuclease (Nase). The products from the Nase digestion were determined by high-performance liquid chromatography (HPLC) to define the extent of reaction with DAO. Mostly, nucleoside 3'-monophosphates were obtained, along with four Nase-resistant dinucleotides: TpdGp, dApdGp, TpdCp-DAO and dApdCp-DAO. The addition of spleen phosphodiesterase II gave a faster hydrolysis and left no dinucleotides. A mixture of Nase, snake venom phosphodiesterase I and alkaline phosphatase gave a fast hydrolysis as well but two dinucleotides, apparently TpdC-DAO and dApdC-DAO, persisted. Further modification of the diaminooctyl side chains with fluorescein isothiocyanate or biotin N-hydroxysuccinimide ester was similarly investigated. Interestingly, derivatization of the DAO side chain with biotin eliminates the resistance of TpdCp-DAO and dApdCp-DAO to Nase digestion. This work provides some guidelines for using Nase, alone or with other nucleases, along with HPLC, for characterizing alkyldiamine DNA products, and should be similarly useful for studying other modifications of DNA." @default.
- W1976562425 created "2016-06-24" @default.
- W1976562425 creator A5002886114 @default.
- W1976562425 creator A5029267240 @default.
- W1976562425 creator A5043574267 @default.
- W1976562425 date "1988-07-01" @default.
- W1976562425 modified "2023-10-16" @default.
- W1976562425 title "Staphylococcal nuclease high-performance liquid chromatographic characterization of diaminooctane-modified DNA and its biotin and fluorescein derivatives" @default.
- W1976562425 cites W1518257019 @default.
- W1976562425 cites W1962263031 @default.
- W1976562425 cites W1965866681 @default.
- W1976562425 cites W1991438804 @default.
- W1976562425 cites W2001015256 @default.
- W1976562425 cites W2026572579 @default.
- W1976562425 cites W2063330692 @default.
- W1976562425 cites W2065595329 @default.
- W1976562425 cites W2077091317 @default.
- W1976562425 cites W2082836578 @default.
- W1976562425 cites W2084129060 @default.
- W1976562425 cites W2089923486 @default.
- W1976562425 cites W2089936728 @default.
- W1976562425 cites W2094334619 @default.
- W1976562425 cites W2746247046 @default.
- W1976562425 doi "https://doi.org/10.1016/s0021-9673(01)94012-9" @default.
- W1976562425 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2849610" @default.
- W1976562425 hasPublicationYear "1988" @default.
- W1976562425 type Work @default.
- W1976562425 sameAs 1976562425 @default.
- W1976562425 citedByCount "5" @default.
- W1976562425 countsByYear W19765624252019 @default.
- W1976562425 crossrefType "journal-article" @default.
- W1976562425 hasAuthorship W1976562425A5002886114 @default.
- W1976562425 hasAuthorship W1976562425A5029267240 @default.
- W1976562425 hasAuthorship W1976562425A5043574267 @default.
- W1976562425 hasConcept C121332964 @default.
- W1976562425 hasConcept C145782189 @default.
- W1976562425 hasConcept C179998833 @default.
- W1976562425 hasConcept C181199279 @default.
- W1976562425 hasConcept C185592680 @default.
- W1976562425 hasConcept C2776923573 @default.
- W1976562425 hasConcept C2777271071 @default.
- W1976562425 hasConcept C2778015030 @default.
- W1976562425 hasConcept C2778204606 @default.
- W1976562425 hasConcept C43617362 @default.
- W1976562425 hasConcept C552990157 @default.
- W1976562425 hasConcept C55493867 @default.
- W1976562425 hasConcept C62520636 @default.
- W1976562425 hasConcept C62826618 @default.
- W1976562425 hasConcept C91881484 @default.
- W1976562425 hasConcept C94412978 @default.
- W1976562425 hasConceptScore W1976562425C121332964 @default.
- W1976562425 hasConceptScore W1976562425C145782189 @default.
- W1976562425 hasConceptScore W1976562425C179998833 @default.
- W1976562425 hasConceptScore W1976562425C181199279 @default.
- W1976562425 hasConceptScore W1976562425C185592680 @default.
- W1976562425 hasConceptScore W1976562425C2776923573 @default.
- W1976562425 hasConceptScore W1976562425C2777271071 @default.
- W1976562425 hasConceptScore W1976562425C2778015030 @default.
- W1976562425 hasConceptScore W1976562425C2778204606 @default.
- W1976562425 hasConceptScore W1976562425C43617362 @default.
- W1976562425 hasConceptScore W1976562425C552990157 @default.
- W1976562425 hasConceptScore W1976562425C55493867 @default.
- W1976562425 hasConceptScore W1976562425C62520636 @default.
- W1976562425 hasConceptScore W1976562425C62826618 @default.
- W1976562425 hasConceptScore W1976562425C91881484 @default.
- W1976562425 hasConceptScore W1976562425C94412978 @default.
- W1976562425 hasLocation W19765624251 @default.
- W1976562425 hasLocation W19765624252 @default.
- W1976562425 hasOpenAccess W1976562425 @default.
- W1976562425 hasPrimaryLocation W19765624251 @default.
- W1976562425 hasRelatedWork W1986395714 @default.
- W1976562425 hasRelatedWork W1991580513 @default.
- W1976562425 hasRelatedWork W2026165519 @default.
- W1976562425 hasRelatedWork W2086107997 @default.
- W1976562425 hasRelatedWork W2096641431 @default.
- W1976562425 hasRelatedWork W2318469034 @default.
- W1976562425 hasRelatedWork W2347937973 @default.
- W1976562425 hasRelatedWork W2388634583 @default.
- W1976562425 hasRelatedWork W2429301418 @default.
- W1976562425 hasRelatedWork W2907045507 @default.
- W1976562425 hasVolume "444" @default.
- W1976562425 isParatext "false" @default.
- W1976562425 isRetracted "false" @default.
- W1976562425 magId "1976562425" @default.
- W1976562425 workType "article" @default.