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• W1976581907 startingPage "1528" @default.
• W1976581907 abstract "The physiological relevance of contacts in crystal lattices often remains elusive. This was also the case for the complex between the invasion protein internalin B (InlB) from Listeria monocytogenes and its host cell receptor, the human receptor tyrosine kinase (RTK) MET. InlB is a MET agonist and induces bacterial host cell invasion. Activation of RTKs generally involves ligand-induced dimerization of the receptor ectodomain. The two currently available crystal structures of the InlB:MET complex show the same arrangement of InlB and MET in a 1:1 complex, but different dimeric 2:2 assemblies. Only one of these 2:2 assemblies is predicted to be stable by a computational procedure. This assembly is mainly stabilized by a contact between the Cap domain of InlB from one and the Sema domain of MET from another 1:1 complex. Here, we probe the physiological relevance of this interaction. We generated variants of the leucine-rich repeat (LRR) protein InlB by inserting an additional repeat between the first and the second LRR. This should allow formation of the 1:1 complex but disrupt the potential 2:2 complex involving the Cap-Sema contact due to steric distortions. A crystal structure of one of the engineered proteins showed that it folded properly. Binding affinity to MET was comparable to that of wild-type InlB. The InlB variant induced MET phosphorylation and cell scatter like wild-type InlB. These results suggest that the Cap-Sema interaction is not physiologically relevant and support the previously proposed assembly, in which a 2:2 InlB:MET complex is built around a ligand dimer." @default.
• W1976581907 created "2016-06-24" @default.
• W1976581907 creator A5009688263 @default.
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• W1976581907 creator A5044718347 @default.
• W1976581907 creator A5071609475 @default.
• W1976581907 date "2012-09-17" @default.
• W1976581907 modified "2023-09-26" @default.
• W1976581907 title "Engineered variants of InlB with an additional leucine-rich repeat discriminate between physiologically relevant and packing contacts in crystal structures of the InlB:MET complex" @default.
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• W1976581907 doi "https://doi.org/10.1002/pro.2142" @default.
• W1976581907 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3526994" @default.
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• W1976581907 hasPublicationYear "2012" @default.
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