FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1976605087> ?p ?o ?g. }

• W1976605087 endingPage "939" @default.
• W1976605087 startingPage "931" @default.
• W1976605087 abstract "OBJECTIVE In this study, we investigated the feasibility of a double-suicide gene/prodrug therapy, involving direct introduction of the herpes simplex virus Type 1 thymidine kinase (TK) gene and the Escherichia coli cytosine deaminase (CD) gene, via a recombinant adenoviral vector, and ganciclovir (GCV) and/or 5-fluorocytosine (5-FC) treatment, in a rat C6 glioma model. METHODS Efficient gene transfer and transduction of C6 glioma cells via a recombinant adenovirus were evaluated by infecting cells with adenovirus bearing the β-galactosidase gene and then staining cells with X-5-bromo-4-chloro-3-indolyl-13-d-galactoside. CD/TK expression in cells infected with adenovirus bearing the CD/TK gene (ad-CD/TK) was examined by immunoblotting analysis. For in vitro cytotoxicity experiments, the cells were infected with ad-CD/TK or ad-ΔE1 (as a control). After the addition of a variety of concentrations of GCV and 5-FC, either separately or in combination, cell viability was determined by staining the cells with crystal violet solution 6 days after infection. For in vivo antitumor experiments, 1 × 105 cells were stereotactically injected into the right caudate-putamen of female Wistar rat brains. At 3 days after implantation, 1 × 108 plaque-forming units of ad-CD/TK or ad-ΔE1 (as a control) were stereotactically injected into the tumors and GCV (25 mg/kg) and 5-FC (250 mg/kg), alone or in combination, were intraperitoneally administered. Animals were then killed, and tumor volumes were measured by determining the tumor area in every fifth section, using a light microscope. RESULTS C6 glioma cells were efficiently transduced with recombinant adenoviral vector at multiplicities of infection of 200 or more. In vitro cytotoxicity of GCV and/or 5-FC, either alone or in combination, was exclusively observed in the cells transduced with ad-CD/TK. Obvious cytotoxicity (>50% inhibition) was observed in the presence of 5-FC at concentrations greater than 30 μg/ml or GCV at concentrations greater than 0.3 μg/ml at a multiplicity of infection of 100. Additionally, cytotoxicity in the presence of both GCV and 5-FC was greater than that after single-prodrug treatments, indicating additive effects of the prodrug treatments. In in vivo experiments, the tumor volumes of the rats treated with GCV or 5-FC alone after ad-CD/TK injection (59.1 ± 4.6 and 57.4 ± 7.1 mm3, respectively) were significantly smaller than that of the control rats (157 ± 8.9 mm3, P < 0.05). Furthermore, the tumor volume of the rats treated with GCV and 5-FC in combination was 14.7 ± 1.8 mm3. CONCLUSION These results demonstrated the efficient transduction of C6 glioma cells with a recombinant adenovirus and the additive effects of CD/TK fusion gene/GCV/5-FC treatment, compared with single-gene therapy with the TK or CD gene. Therefore, our data suggest that the direct administration of a double-suicide gene/prodrug therapy has great potential in the treatment of brain tumors." @default.
• W1976605087 created "2016-06-24" @default.
• W1976605087 creator A5003819949 @default.
• W1976605087 creator A5005358526 @default.
• W1976605087 creator A5014132243 @default.
• W1976605087 creator A5014234255 @default.
• W1976605087 creator A5043499449 @default.
• W1976605087 creator A5046007610 @default.
• W1976605087 date "2000-10-01" @default.
• W1976605087 modified "2023-10-11" @default.
• W1976605087 title "Combined Antitumor Effects of an Adenoviral Cytosine Deaminase/Thymidine Kinase Fusion Gene in Rat C6 Glioma" @default.
• W1976605087 cites W1964863866 @default.
• W1976605087 cites W1965372133 @default.
• W1976605087 cites W1976435658 @default.
• W1976605087 cites W2000269821 @default.
• W1976605087 cites W2006149037 @default.
• W1976605087 cites W2012315955 @default.
• W1976605087 cites W2026922744 @default.
• W1976605087 cites W2034108248 @default.
• W1976605087 cites W2045001483 @default.
• W1976605087 cites W2047275511 @default.
• W1976605087 cites W2051033051 @default.
• W1976605087 cites W2065915399 @default.
• W1976605087 cites W2078521670 @default.
• W1976605087 cites W2088058542 @default.
• W1976605087 cites W2165699027 @default.
• W1976605087 cites W2332749011 @default.
• W1976605087 cites W2416263105 @default.
• W1976605087 cites W2418972175 @default.
• W1976605087 cites W9043722 @default.
• W1976605087 doi "https://doi.org/10.1097/00006123-200010000-00026" @default.
• W1976605087 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11014433" @default.
• W1976605087 hasPublicationYear "2000" @default.
• W1976605087 type Work @default.
• W1976605087 sameAs 1976605087 @default.
• W1976605087 citedByCount "22" @default.
• W1976605087 countsByYear W19766050872012 @default.
• W1976605087 countsByYear W19766050872013 @default.
• W1976605087 countsByYear W19766050872017 @default.
• W1976605087 countsByYear W19766050872018 @default.
• W1976605087 countsByYear W19766050872019 @default.
• W1976605087 crossrefType "journal-article" @default.
• W1976605087 hasAuthorship W1976605087A5003819949 @default.
• W1976605087 hasAuthorship W1976605087A5005358526 @default.
• W1976605087 hasAuthorship W1976605087A5014132243 @default.
• W1976605087 hasAuthorship W1976605087A5014234255 @default.
• W1976605087 hasAuthorship W1976605087A5043499449 @default.
• W1976605087 hasAuthorship W1976605087A5046007610 @default.
• W1976605087 hasConcept C104317684 @default.
• W1976605087 hasConcept C111599444 @default.
• W1976605087 hasConcept C153911025 @default.
• W1976605087 hasConcept C159047783 @default.
• W1976605087 hasConcept C2522874641 @default.
• W1976605087 hasConcept C2776147627 @default.
• W1976605087 hasConcept C2776954459 @default.
• W1976605087 hasConcept C2777748644 @default.
• W1976605087 hasConcept C2777965375 @default.
• W1976605087 hasConcept C2778227246 @default.
• W1976605087 hasConcept C2779820661 @default.
• W1976605087 hasConcept C2781196997 @default.
• W1976605087 hasConcept C32470452 @default.
• W1976605087 hasConcept C34981463 @default.
• W1976605087 hasConcept C40767141 @default.
• W1976605087 hasConcept C502942594 @default.
• W1976605087 hasConcept C54355233 @default.
• W1976605087 hasConcept C55493867 @default.
• W1976605087 hasConcept C71924100 @default.
• W1976605087 hasConcept C81885089 @default.
• W1976605087 hasConcept C86803240 @default.
• W1976605087 hasConceptScore W1976605087C104317684 @default.
• W1976605087 hasConceptScore W1976605087C111599444 @default.
• W1976605087 hasConceptScore W1976605087C153911025 @default.
• W1976605087 hasConceptScore W1976605087C159047783 @default.
• W1976605087 hasConceptScore W1976605087C2522874641 @default.
• W1976605087 hasConceptScore W1976605087C2776147627 @default.
• W1976605087 hasConceptScore W1976605087C2776954459 @default.
• W1976605087 hasConceptScore W1976605087C2777748644 @default.
• W1976605087 hasConceptScore W1976605087C2777965375 @default.
• W1976605087 hasConceptScore W1976605087C2778227246 @default.
• W1976605087 hasConceptScore W1976605087C2779820661 @default.
• W1976605087 hasConceptScore W1976605087C2781196997 @default.
• W1976605087 hasConceptScore W1976605087C32470452 @default.
• W1976605087 hasConceptScore W1976605087C34981463 @default.
• W1976605087 hasConceptScore W1976605087C40767141 @default.
• W1976605087 hasConceptScore W1976605087C502942594 @default.
• W1976605087 hasConceptScore W1976605087C54355233 @default.
• W1976605087 hasConceptScore W1976605087C55493867 @default.
• W1976605087 hasConceptScore W1976605087C71924100 @default.
• W1976605087 hasConceptScore W1976605087C81885089 @default.
• W1976605087 hasConceptScore W1976605087C86803240 @default.
• W1976605087 hasIssue "4" @default.
• W1976605087 hasLocation W19766050871 @default.
• W1976605087 hasLocation W19766050872 @default.
• W1976605087 hasOpenAccess W1976605087 @default.
• W1976605087 hasPrimaryLocation W19766050871 @default.
• W1976605087 hasRelatedWork W1498422737 @default.
• W1976605087 hasRelatedWork W1963729009 @default.
• W1976605087 hasRelatedWork W1979990769 @default.

• next