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• W1976656687 endingPage "138" @default.
• W1976656687 startingPage "133" @default.
• W1976656687 abstract "Clostridium difficile is a leading cause of healthcare-associated diarrhoea. In recent years, certain C. difficile types have become highly represented among clinical isolates and are associated with outbreaks of increased disease severity, higher relapse rates and an expanded repertoire of antibiotic resistance. Endospores, produced during sporulation, play a pivotal role in infection and disease transmission and it has been suggested in the literature that these so-called ‘hypervirulent’ C. difficile types are more prolific in terms of sporulation in vitro. However, work in our laboratory has provided evidence to the contrary suggesting that although there is significant strain-to-strain variation in C. difficile sporulation characteristics this variation does not appear to be type-associated. On analysis of the literature, it is apparent that the methods used to quantify sporulation in previous studies have varied greatly and sample sizes have remained small. The conflicting data in the literature may, therefore, not necessarily be generally representative of C. difficile sporulation. Instead, these inconsistencies may reflect differences in the experimental design of each study. In this review, the need for further investigations of C. difficile sporulation rates is highlighted. Specifically, the advantages and disadvantages of the different experimental approaches previously used are discussed and a standard set of principles for measuring C. difficile sporulation in the future is proposed." @default.
• W1976656687 created "2016-06-24" @default.
• W1976656687 creator A5044206227 @default.
• W1976656687 creator A5044949409 @default.
• W1976656687 date "2011-11-01" @default.
• W1976656687 modified "2023-09-25" @default.
• W1976656687 title "Sporulation studies in Clostridium difficile" @default.
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• W1976656687 doi "https://doi.org/10.1016/j.mimet.2011.07.017" @default.
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