FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1976710366> ?p ?o ?g. }

• W1976710366 endingPage "879" @default.
• W1976710366 startingPage "870" @default.
• W1976710366 abstract "The mechanism of DNA polymerase β-catalyzed nucleotidyl transfer consists of chemical steps involving primer 3‘ OH deprotonation, nucleophilic attack, and pyrophosphate leaving-group elimination, preceded by dNTP binding which induces a large-amplitude conformational change for Watson−Crick nascent base pairs. Ambiguity in the nature of the rate-limiting step and active-site structural differences between correct and incorrect base-paired transition states remain obstacles to understanding DNA replication fidelity. Analogues of dGTP where the β-γ bridging oxygen is replaced with fluorine-substituted methylene groups have been shown to probe the contribution of leaving-group elimination to the overall catalytic rate (Biochemistry 46, 461−471). Here, the analysis is expanded substantially to include a broad range of halogen substituents with disparate steric and electronic properties. Evaluation of linear free energy relationships for incorporation of dGTP analogues opposite either template base C or T reveals a strong correlation of log(kpol) to leaving group pKa. Significantly different kpol behavior is observed with a subset of the analogues, with magnitude dependent on the identity of the nascent base pair. This observation, and the absence of an analogous effect on ground state analogue binding (Kd values), points to active-site structural differences at the chemical transition state. Reduced catalysis with bulky halo-containing substrates is manifested in the fidelity of T-G incorporation, where the CCl2-bridging analogue shows a 27-fold increase in fidelity over the natural dGTP. Solvent pH and deuterium isotope-effect data are also used to evaluate mechanistic differences between correct and mispaired incorporation." @default.
• W1976710366 created "2016-06-24" @default.
• W1976710366 creator A5011933351 @default.
• W1976710366 creator A5039592552 @default.
• W1976710366 creator A5041489306 @default.
• W1976710366 creator A5045523035 @default.
• W1976710366 creator A5055219169 @default.
• W1976710366 creator A5061042545 @default.
• W1976710366 creator A5062571628 @default.
• W1976710366 creator A5067404614 @default.
• W1976710366 creator A5081702583 @default.
• W1976710366 creator A5085922344 @default.
• W1976710366 creator A5088665303 @default.
• W1976710366 date "2007-12-28" @default.
• W1976710366 modified "2023-10-02" @default.
• W1976710366 title "DNA Polymerase β Fidelity: Halomethylene-Modified Leaving Groups in Pre-Steady-State Kinetic Analysis Reveal Differences at the Chemical Transition State" @default.
• W1976710366 cites W1499552914 @default.
• W1976710366 cites W1870697906 @default.
• W1976710366 cites W1980043549 @default.
• W1976710366 cites W1984478391 @default.
• W1976710366 cites W1993620005 @default.
• W1976710366 cites W1998804839 @default.
• W1976710366 cites W2046290782 @default.
• W1976710366 cites W2053895127 @default.
• W1976710366 cites W2073875893 @default.
• W1976710366 cites W2111609734 @default.
• W1976710366 cites W2125775090 @default.
• W1976710366 doi "https://doi.org/10.1021/bi7014162" @default.
• W1976710366 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18161950" @default.
• W1976710366 hasPublicationYear "2007" @default.
• W1976710366 type Work @default.
• W1976710366 sameAs 1976710366 @default.
• W1976710366 citedByCount "78" @default.
• W1976710366 countsByYear W19767103662012 @default.
• W1976710366 countsByYear W19767103662013 @default.
• W1976710366 countsByYear W19767103662014 @default.
• W1976710366 countsByYear W19767103662015 @default.
• W1976710366 countsByYear W19767103662016 @default.
• W1976710366 countsByYear W19767103662017 @default.
• W1976710366 countsByYear W19767103662018 @default.
• W1976710366 countsByYear W19767103662019 @default.
• W1976710366 countsByYear W19767103662020 @default.
• W1976710366 countsByYear W19767103662021 @default.
• W1976710366 countsByYear W19767103662022 @default.
• W1976710366 countsByYear W19767103662023 @default.
• W1976710366 crossrefType "journal-article" @default.
• W1976710366 hasAuthorship W1976710366A5011933351 @default.
• W1976710366 hasAuthorship W1976710366A5039592552 @default.
• W1976710366 hasAuthorship W1976710366A5041489306 @default.
• W1976710366 hasAuthorship W1976710366A5045523035 @default.
• W1976710366 hasAuthorship W1976710366A5055219169 @default.
• W1976710366 hasAuthorship W1976710366A5061042545 @default.
• W1976710366 hasAuthorship W1976710366A5062571628 @default.
• W1976710366 hasAuthorship W1976710366A5067404614 @default.
• W1976710366 hasAuthorship W1976710366A5081702583 @default.
• W1976710366 hasAuthorship W1976710366A5085922344 @default.
• W1976710366 hasAuthorship W1976710366A5088665303 @default.
• W1976710366 hasConcept C121332964 @default.
• W1976710366 hasConcept C160964918 @default.
• W1976710366 hasConcept C161790260 @default.
• W1976710366 hasConcept C178790620 @default.
• W1976710366 hasConcept C185581394 @default.
• W1976710366 hasConcept C185592680 @default.
• W1976710366 hasConcept C201194858 @default.
• W1976710366 hasConcept C552990157 @default.
• W1976710366 hasConcept C55493867 @default.
• W1976710366 hasConcept C58364064 @default.
• W1976710366 hasConcept C62520636 @default.
• W1976710366 hasConcept C69928629 @default.
• W1976710366 hasConcept C71240020 @default.
• W1976710366 hasConceptScore W1976710366C121332964 @default.
• W1976710366 hasConceptScore W1976710366C160964918 @default.
• W1976710366 hasConceptScore W1976710366C161790260 @default.
• W1976710366 hasConceptScore W1976710366C178790620 @default.
• W1976710366 hasConceptScore W1976710366C185581394 @default.
• W1976710366 hasConceptScore W1976710366C185592680 @default.
• W1976710366 hasConceptScore W1976710366C201194858 @default.
• W1976710366 hasConceptScore W1976710366C552990157 @default.
• W1976710366 hasConceptScore W1976710366C55493867 @default.
• W1976710366 hasConceptScore W1976710366C58364064 @default.
• W1976710366 hasConceptScore W1976710366C62520636 @default.
• W1976710366 hasConceptScore W1976710366C69928629 @default.
• W1976710366 hasConceptScore W1976710366C71240020 @default.
• W1976710366 hasIssue "3" @default.
• W1976710366 hasLocation W19767103661 @default.
• W1976710366 hasLocation W19767103662 @default.
• W1976710366 hasOpenAccess W1976710366 @default.
• W1976710366 hasPrimaryLocation W19767103661 @default.
• W1976710366 hasRelatedWork W1995425375 @default.
• W1976710366 hasRelatedWork W2016943609 @default.
• W1976710366 hasRelatedWork W2033094300 @default.
• W1976710366 hasRelatedWork W2033358700 @default.
• W1976710366 hasRelatedWork W2059608682 @default.
• W1976710366 hasRelatedWork W2061113752 @default.
• W1976710366 hasRelatedWork W2077034711 @default.
• W1976710366 hasRelatedWork W2097483748 @default.
• W1976710366 hasRelatedWork W2102293398 @default.
• W1976710366 hasRelatedWork W2950814935 @default.

• next