FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1976736698> ?p ?o ?g. }

• W1976736698 endingPage "e0124736" @default.
• W1976736698 startingPage "e0124736" @default.
• W1976736698 abstract "Alzheimer’s disease (AD) is the most common form of age-related dementia, and the most urgent problem is that it is currently incurable. Amyloid-β (Aβ) peptide is believed to play a major role in the pathogenesis of AD. We previously reported that an Aβ N-terminal amino acid targeting monoclonal antibody (MAb), A8, inhibits Aβ fibril formation and has potential as an immunotherapy for AD based on a mouse model. To further study the underlying mechanisms, we tested our hypothesis that the single chain fragment variable (scFv) without the Fc fragment is capable of regulating either Aβ aggregation or disaggregation in vitro. Here, a model of cell-free Aβ “on-pathway” aggregation was established and identified using PCR, Western blot, ELISA, transmission electron microscopy (TEM) and thioflavin T (ThT) binding analyses. His-tagged A8 scFvs was cloned and solubly expressed in baculovirus. Our data demonstrated that the Ni-NTA agarose affinity-purified A8 scFv inhibited the forward reaction of “on-pathway” aggregation and Aβ fibril maturation. The effect of A8 scFv on Aβ fibrillogenesis was markedly more significant when administered at the start of the Aβ folding reaction. Furthermore, the results also showed that pre-formed Aβ fibrils could be disaggregated via incubation with purified A8 scFv, which suggested that A8 scFv is involved in the reverse reaction of Aβ aggregation. Therefore, A8 scFv was capable of both inhibiting fibrillogenesis and disaggregating matured fibrils. Our present study provides valuable insight into the regulators of ultrastructural dynamics of cell-free “on-pathway” Aβ aggregation and will assist in the development of therapeutic strategies for AD." @default.
• W1976736698 created "2016-06-24" @default.
• W1976736698 creator A5042241049 @default.
• W1976736698 creator A5043490198 @default.
• W1976736698 creator A5045705855 @default.
• W1976736698 creator A5051555994 @default.
• W1976736698 creator A5054908805 @default.
• W1976736698 creator A5059179466 @default.
• W1976736698 creator A5059235151 @default.
• W1976736698 creator A5069077456 @default.
• W1976736698 creator A5074031875 @default.
• W1976736698 creator A5074813385 @default.
• W1976736698 creator A5081609966 @default.
• W1976736698 date "2015-04-28" @default.
• W1976736698 modified "2023-09-27" @default.
• W1976736698 title "Single Chain Variable Fragment Against Aβ Expressed in Baculovirus Inhibits Abeta Fibril Elongation and Promotes its Disaggregation" @default.
• W1976736698 cites W1268992491 @default.
• W1976736698 cites W1605023308 @default.
• W1976736698 cites W1607954709 @default.
• W1976736698 cites W1608225731 @default.
• W1976736698 cites W1964622632 @default.
• W1976736698 cites W1970916286 @default.
• W1976736698 cites W1992045133 @default.
• W1976736698 cites W1992474532 @default.
• W1976736698 cites W2000917982 @default.
• W1976736698 cites W2013196885 @default.
• W1976736698 cites W2029181535 @default.
• W1976736698 cites W2035202576 @default.
• W1976736698 cites W2039956388 @default.
• W1976736698 cites W2044098401 @default.
• W1976736698 cites W2045117614 @default.
• W1976736698 cites W2045590748 @default.
• W1976736698 cites W2053571233 @default.
• W1976736698 cites W2055609315 @default.
• W1976736698 cites W2056241835 @default.
• W1976736698 cites W2062438517 @default.
• W1976736698 cites W2063808031 @default.
• W1976736698 cites W2069402857 @default.
• W1976736698 cites W2080065760 @default.
• W1976736698 cites W2097631517 @default.
• W1976736698 cites W2099540110 @default.
• W1976736698 cites W2126680935 @default.
• W1976736698 cites W2145629698 @default.
• W1976736698 cites W2154404562 @default.
• W1976736698 cites W2156037313 @default.
• W1976736698 doi "https://doi.org/10.1371/journal.pone.0124736" @default.
• W1976736698 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4412524" @default.
• W1976736698 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25919299" @default.
• W1976736698 hasPublicationYear "2015" @default.
• W1976736698 type Work @default.
• W1976736698 sameAs 1976736698 @default.
• W1976736698 citedByCount "6" @default.
• W1976736698 countsByYear W19767366982016 @default.
• W1976736698 countsByYear W19767366982017 @default.
• W1976736698 countsByYear W19767366982020 @default.
• W1976736698 countsByYear W19767366982021 @default.
• W1976736698 crossrefType "journal-article" @default.
• W1976736698 hasAuthorship W1976736698A5042241049 @default.
• W1976736698 hasAuthorship W1976736698A5043490198 @default.
• W1976736698 hasAuthorship W1976736698A5045705855 @default.
• W1976736698 hasAuthorship W1976736698A5051555994 @default.
• W1976736698 hasAuthorship W1976736698A5054908805 @default.
• W1976736698 hasAuthorship W1976736698A5059179466 @default.
• W1976736698 hasAuthorship W1976736698A5059235151 @default.
• W1976736698 hasAuthorship W1976736698A5069077456 @default.
• W1976736698 hasAuthorship W1976736698A5074031875 @default.
• W1976736698 hasAuthorship W1976736698A5074813385 @default.
• W1976736698 hasAuthorship W1976736698A5081609966 @default.
• W1976736698 hasBestOaLocation W19767366981 @default.
• W1976736698 hasConcept C104317684 @default.
• W1976736698 hasConcept C12554922 @default.
• W1976736698 hasConcept C136238340 @default.
• W1976736698 hasConcept C142724271 @default.
• W1976736698 hasConcept C147259501 @default.
• W1976736698 hasConcept C153911025 @default.
• W1976736698 hasConcept C179104552 @default.
• W1976736698 hasConcept C185592680 @default.
• W1976736698 hasConcept C27523624 @default.
• W1976736698 hasConcept C2776967038 @default.
• W1976736698 hasConcept C2777633098 @default.
• W1976736698 hasConcept C2778896982 @default.
• W1976736698 hasConcept C2779134260 @default.
• W1976736698 hasConcept C40767141 @default.
• W1976736698 hasConcept C502032728 @default.
• W1976736698 hasConcept C55493867 @default.
• W1976736698 hasConcept C71924100 @default.
• W1976736698 hasConcept C86803240 @default.
• W1976736698 hasConcept C95444343 @default.
• W1976736698 hasConceptScore W1976736698C104317684 @default.
• W1976736698 hasConceptScore W1976736698C12554922 @default.
• W1976736698 hasConceptScore W1976736698C136238340 @default.
• W1976736698 hasConceptScore W1976736698C142724271 @default.
• W1976736698 hasConceptScore W1976736698C147259501 @default.
• W1976736698 hasConceptScore W1976736698C153911025 @default.
• W1976736698 hasConceptScore W1976736698C179104552 @default.
• W1976736698 hasConceptScore W1976736698C185592680 @default.
• W1976736698 hasConceptScore W1976736698C27523624 @default.
• W1976736698 hasConceptScore W1976736698C2776967038 @default.

• next