Matches in SemOpenAlex for { <https://semopenalex.org/work/W1976796952> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W1976796952 endingPage "146" @default.
- W1976796952 startingPage "146" @default.
- W1976796952 abstract "Somatic cell nuclear transfer (SCNT) offers the possibility of pig transgenesis. Importantly, genetic manipulations can be performed in cells isolated from special breeds followed by SCNT into enucleated oocytes isolated from slaughterhouse ovaries. In the present study, we established production of Yucatan blastocysts by the handmade cloning (HMC) technique using non-transgenic fibroblasts from the Yucatan miniature pig, and produced transgenic blastocysts using enhanced green fluorescent protein (EGFP)-positive Yucatan fetal fibroblasts. For transgenesis, Yucatan fibroblasts from a 40-day old fetus were transfected with a vector containing an EGFP gene and a neomycin-resistance selection gene by lipofection. Well separated neomycin-resistant colonies were isolated, expanded, and used for HMC. For HMC, cumulus–oocyte complexes were aspirated from ovaries of slaughterhouse sows and matured for 41 h. Subsequently, the cumulus cells were removed in hyaluronidase, and zonae pellucidae were partially digested by incubation in pronase. Oocytes with a visible polar body (PB) were subjected to oriented bisection. Less than half of the cytoplasm adjacent to the PB was removed with a microblade. The remaining parts, i.e. cytoplasts, were used as recipients for embryo reconstruction. Reconstructed embryos were produced by a two-step fusion procedure. At the first step, one cytoplast was fused with one fibroblast in the absence of Ca2+. After one h, the cytoplast-fibroblast pair and another cytoplast were fused and activated simultaneously in the presence of Ca2+, and subsequently cultured in cytochalasin B and cycloheximide for 4 h. The development of reconstructed embryos to the blastocyst stage was determined after 7 days of in vitro culture. When using non-transgenic and EGFP-positive Yucatan fetal fibroblasts, the rate of blastocyst formation (mean � SEM) were 36 � 7% (36/102) and 42 � 7% (32/77), respectively. In conclusion, the HMC technique was very efficient for production of blastocysts of Yucatan miniature pig origin using both non-transgenic and EGFP-positive fetal fibroblasts." @default.
- W1976796952 created "2016-06-24" @default.
- W1976796952 creator A5018112499 @default.
- W1976796952 creator A5046382012 @default.
- W1976796952 creator A5059888564 @default.
- W1976796952 creator A5073539033 @default.
- W1976796952 creator A5075702310 @default.
- W1976796952 creator A5081248289 @default.
- W1976796952 date "2007-01-01" @default.
- W1976796952 modified "2023-09-25" @default.
- W1976796952 title "56 TRANSGENESIS BY HANDMADE CLONING USING EGFP-TRANSFECTED YUCATAN FIBROBLASTS" @default.
- W1976796952 doi "https://doi.org/10.1071/rdv19n1ab56" @default.
- W1976796952 hasPublicationYear "2007" @default.
- W1976796952 type Work @default.
- W1976796952 sameAs 1976796952 @default.
- W1976796952 citedByCount "0" @default.
- W1976796952 crossrefType "journal-article" @default.
- W1976796952 hasAuthorship W1976796952A5018112499 @default.
- W1976796952 hasAuthorship W1976796952A5046382012 @default.
- W1976796952 hasAuthorship W1976796952A5059888564 @default.
- W1976796952 hasAuthorship W1976796952A5073539033 @default.
- W1976796952 hasAuthorship W1976796952A5075702310 @default.
- W1976796952 hasAuthorship W1976796952A5081248289 @default.
- W1976796952 hasConcept C121050878 @default.
- W1976796952 hasConcept C14814345 @default.
- W1976796952 hasConcept C1491633281 @default.
- W1976796952 hasConcept C153911025 @default.
- W1976796952 hasConcept C16685009 @default.
- W1976796952 hasConcept C179933525 @default.
- W1976796952 hasConcept C190546358 @default.
- W1976796952 hasConcept C196843134 @default.
- W1976796952 hasConcept C199360897 @default.
- W1976796952 hasConcept C202438428 @default.
- W1976796952 hasConcept C2778177303 @default.
- W1976796952 hasConcept C2779012151 @default.
- W1976796952 hasConcept C2779335785 @default.
- W1976796952 hasConcept C2780381497 @default.
- W1976796952 hasConcept C2994278967 @default.
- W1976796952 hasConcept C41008148 @default.
- W1976796952 hasConcept C54355233 @default.
- W1976796952 hasConcept C71924100 @default.
- W1976796952 hasConcept C73247094 @default.
- W1976796952 hasConcept C81885089 @default.
- W1976796952 hasConcept C86803240 @default.
- W1976796952 hasConcept C87073359 @default.
- W1976796952 hasConcept C95444343 @default.
- W1976796952 hasConceptScore W1976796952C121050878 @default.
- W1976796952 hasConceptScore W1976796952C14814345 @default.
- W1976796952 hasConceptScore W1976796952C1491633281 @default.
- W1976796952 hasConceptScore W1976796952C153911025 @default.
- W1976796952 hasConceptScore W1976796952C16685009 @default.
- W1976796952 hasConceptScore W1976796952C179933525 @default.
- W1976796952 hasConceptScore W1976796952C190546358 @default.
- W1976796952 hasConceptScore W1976796952C196843134 @default.
- W1976796952 hasConceptScore W1976796952C199360897 @default.
- W1976796952 hasConceptScore W1976796952C202438428 @default.
- W1976796952 hasConceptScore W1976796952C2778177303 @default.
- W1976796952 hasConceptScore W1976796952C2779012151 @default.
- W1976796952 hasConceptScore W1976796952C2779335785 @default.
- W1976796952 hasConceptScore W1976796952C2780381497 @default.
- W1976796952 hasConceptScore W1976796952C2994278967 @default.
- W1976796952 hasConceptScore W1976796952C41008148 @default.
- W1976796952 hasConceptScore W1976796952C54355233 @default.
- W1976796952 hasConceptScore W1976796952C71924100 @default.
- W1976796952 hasConceptScore W1976796952C73247094 @default.
- W1976796952 hasConceptScore W1976796952C81885089 @default.
- W1976796952 hasConceptScore W1976796952C86803240 @default.
- W1976796952 hasConceptScore W1976796952C87073359 @default.
- W1976796952 hasConceptScore W1976796952C95444343 @default.
- W1976796952 hasIssue "1" @default.
- W1976796952 hasLocation W19767969521 @default.
- W1976796952 hasOpenAccess W1976796952 @default.
- W1976796952 hasPrimaryLocation W19767969521 @default.
- W1976796952 hasRelatedWork W1567668280 @default.
- W1976796952 hasRelatedWork W1976796952 @default.
- W1976796952 hasRelatedWork W2004306661 @default.
- W1976796952 hasRelatedWork W2007826443 @default.
- W1976796952 hasRelatedWork W2037298458 @default.
- W1976796952 hasRelatedWork W2051022244 @default.
- W1976796952 hasRelatedWork W2071557794 @default.
- W1976796952 hasRelatedWork W2090578102 @default.
- W1976796952 hasRelatedWork W2153522046 @default.
- W1976796952 hasRelatedWork W2584700121 @default.
- W1976796952 hasVolume "19" @default.
- W1976796952 isParatext "false" @default.
- W1976796952 isRetracted "false" @default.
- W1976796952 magId "1976796952" @default.
- W1976796952 workType "article" @default.