Matches in SemOpenAlex for { <https://semopenalex.org/work/W1976827449> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W1976827449 endingPage "553" @default.
- W1976827449 startingPage "546" @default.
- W1976827449 abstract "Three methods are described for quantitative depletion of calf thymus and chicken erythrocyte chromatin of various histone fractions. All methods involve dissociation of the histone fractions in question from chromatin in the presence of the ion-exchange resin AG50W X 2 which, having strongly bound the free histones in solution, is easily removed from the chromatin. The depleted chromatins do not appear to be denatured. Method 1. In 0.45–0.65 M NaCl, 0.05 M sodium phosphate buffer pH 7.0, histone F1 is quantitatively and selectively removed from calf thymus chromatin. In 0.55–0.65 M NaCl, 0.05 M sodium phosphate buffer pH 7.0 and a limiting amount of ion-exchange resin, histone F2C is quantitatively and selectively removed from chicken erythrocyte chromatin. Histone F1 can be quantitatively and selectively removed from chicken erythrocyte chromatin by repeated extraction with 0.5 M NaCl pH 7. By further depletion with resin in 0.55–0.65 M NaCl, 0.05 M sodium phosphate buffer pH 7.0, chicken erythrocyte chromatin can be quantitatively and selectively depleted in both histones F1 and F2C. Method 2. In 0.25–0.45M NaCl, 0.05 M sodium phosphate buffer, 4 M urea pH 7.0, histones F1, F2B and F2A2 are quantitatively and selectively removed from calf thymus chromatin. Method 3. In 0.35 M NaCl, 30% ethanol, 4 M urea pH 7.0, an almost complete removal of histones F2A1 and F2A2 from calf thymus chromatin is achieved. In 0.35 M NaCl, 300% ethanol, 5–6 M urea pH 7.0, histone F3 is removed as well." @default.
- W1976827449 created "2016-06-24" @default.
- W1976827449 creator A5046382012 @default.
- W1976827449 creator A5076617637 @default.
- W1976827449 date "1973-06-01" @default.
- W1976827449 modified "2023-10-01" @default.
- W1976827449 title "The Selective Extraction of Histone Fractions from Deoxyribonucleoprotein" @default.
- W1976827449 cites W1753210391 @default.
- W1976827449 cites W1775749144 @default.
- W1976827449 cites W192545017 @default.
- W1976827449 cites W1965191886 @default.
- W1976827449 cites W1975843199 @default.
- W1976827449 cites W1976913182 @default.
- W1976827449 cites W1980153321 @default.
- W1976827449 cites W1982657684 @default.
- W1976827449 cites W2017470279 @default.
- W1976827449 cites W2026437734 @default.
- W1976827449 cites W2030542163 @default.
- W1976827449 cites W2034935049 @default.
- W1976827449 cites W2039536109 @default.
- W1976827449 cites W2048315728 @default.
- W1976827449 cites W2083752722 @default.
- W1976827449 cites W2088759580 @default.
- W1976827449 cites W2094477858 @default.
- W1976827449 cites W211112821 @default.
- W1976827449 cites W2316838174 @default.
- W1976827449 cites W98864642 @default.
- W1976827449 doi "https://doi.org/10.1111/j.1432-1033.1973.tb02871.x" @default.
- W1976827449 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4738395" @default.
- W1976827449 hasPublicationYear "1973" @default.
- W1976827449 type Work @default.
- W1976827449 sameAs 1976827449 @default.
- W1976827449 citedByCount "86" @default.
- W1976827449 countsByYear W19768274492015 @default.
- W1976827449 countsByYear W19768274492019 @default.
- W1976827449 crossrefType "journal-article" @default.
- W1976827449 hasAuthorship W1976827449A5046382012 @default.
- W1976827449 hasAuthorship W1976827449A5076617637 @default.
- W1976827449 hasBestOaLocation W19768274491 @default.
- W1976827449 hasConcept C12554922 @default.
- W1976827449 hasConcept C178790620 @default.
- W1976827449 hasConcept C185592680 @default.
- W1976827449 hasConcept C2777132085 @default.
- W1976827449 hasConcept C2780365088 @default.
- W1976827449 hasConcept C43617362 @default.
- W1976827449 hasConcept C4725764 @default.
- W1976827449 hasConcept C537181965 @default.
- W1976827449 hasConcept C552990157 @default.
- W1976827449 hasConcept C55493867 @default.
- W1976827449 hasConcept C64927066 @default.
- W1976827449 hasConcept C83640560 @default.
- W1976827449 hasConcept C86803240 @default.
- W1976827449 hasConcept C96616956 @default.
- W1976827449 hasConceptScore W1976827449C12554922 @default.
- W1976827449 hasConceptScore W1976827449C178790620 @default.
- W1976827449 hasConceptScore W1976827449C185592680 @default.
- W1976827449 hasConceptScore W1976827449C2777132085 @default.
- W1976827449 hasConceptScore W1976827449C2780365088 @default.
- W1976827449 hasConceptScore W1976827449C43617362 @default.
- W1976827449 hasConceptScore W1976827449C4725764 @default.
- W1976827449 hasConceptScore W1976827449C537181965 @default.
- W1976827449 hasConceptScore W1976827449C552990157 @default.
- W1976827449 hasConceptScore W1976827449C55493867 @default.
- W1976827449 hasConceptScore W1976827449C64927066 @default.
- W1976827449 hasConceptScore W1976827449C83640560 @default.
- W1976827449 hasConceptScore W1976827449C86803240 @default.
- W1976827449 hasConceptScore W1976827449C96616956 @default.
- W1976827449 hasIssue "3" @default.
- W1976827449 hasLocation W19768274491 @default.
- W1976827449 hasLocation W19768274492 @default.
- W1976827449 hasOpenAccess W1976827449 @default.
- W1976827449 hasPrimaryLocation W19768274491 @default.
- W1976827449 hasRelatedWork W1971223895 @default.
- W1976827449 hasRelatedWork W1997914669 @default.
- W1976827449 hasRelatedWork W2036573365 @default.
- W1976827449 hasRelatedWork W2039193920 @default.
- W1976827449 hasRelatedWork W2062023201 @default.
- W1976827449 hasRelatedWork W2075924981 @default.
- W1976827449 hasRelatedWork W2129029894 @default.
- W1976827449 hasRelatedWork W2175891077 @default.
- W1976827449 hasRelatedWork W2278105877 @default.
- W1976827449 hasRelatedWork W2418988680 @default.
- W1976827449 hasVolume "35" @default.
- W1976827449 isParatext "false" @default.
- W1976827449 isRetracted "false" @default.
- W1976827449 magId "1976827449" @default.
- W1976827449 workType "article" @default.